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    A NOVEL VECTOR AND EXPRESSION CELL LINE FOR MASS PRODUCTION OF RECOMBINANT PROTEIN AND A PROCESS OF PRODUCING RECOMBINANT PROTEIN USING SAME
    1.
    发明申请
    A NOVEL VECTOR AND EXPRESSION CELL LINE FOR MASS PRODUCTION OF RECOMBINANT PROTEIN AND A PROCESS OF PRODUCING RECOMBINANT PROTEIN USING SAME 有权
    用于生产重组蛋白质的新型载体和表达细胞系以及使用其生产重组蛋白的方法

    公开(公告)号：US20100120089A1

    公开(公告)日：2010-05-13

    申请号：US12444567

    申请日：2007-10-16

    申请人： In Young Choi ,  Chang Hwan Kim ,  Hyun Ji Lee ,  Seong Hee Park ,  Se Chang Kwon ,  Gwan Sun Lee

    发明人： In Young Choi ,  Chang Hwan Kim ,  Hyun Ji Lee ,  Seong Hee Park ,  Se Chang Kwon ,  Gwan Sun Lee

    IPC分类号： C12P21/00 ,  C12N15/74 ,  C12N5/10

    CPC分类号： C12N9/003 ,  C12N15/85 ,  C12N2510/00

    摘要： Disclosed herein is an inducible high-expression cassette comprising a dihydrofolate reductase (DHFR) promoter from which GC-rich repeat sequences are partially or entirely removed, the cassette capable of more effectively improving a gene amplification system. Also disclosed are an expression vector comprising the inducible expression cassette and optionally a gene encoding a recombinant protein of interest, an animal cell line transformed with the expression vector, and a method of mass producing and purifying a recombinant protein by culturing the transformant. The present invention enables the shortening of the time required to establish a cell line producing a recombinant protein of interest at high levels using a low concentration of a DHFR inhibitor, thereby allowing more effective production of the recombinant protein.

    摘要翻译： 本文公开了包含二氢叶酸还原酶（DHFR）启动子的诱导型高表达盒，从其中部分或全部除去富含GC的重复序列，所述盒能够更有效地改善基因扩增系统。 还公开了包含可诱导表达盒和任选的编码目标重组蛋白的基因，用表达载体转化的动物细胞系的表达载体，以及通过培养转化体大量生产和纯化重组蛋白的方法。 本发明能够缩短使用低浓度的DHFR抑制剂以高水平建立产生重组蛋白质的细胞系所需的时间，从而允许更有效地产生重组蛋白质。

    Vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same
    2.
    发明授权
    Vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same 有权
    用于大量生产重组蛋白的载体和表达细胞系以及使用其生产重组蛋白的方法

    公开(公告)号：US08957196B2

    公开(公告)日：2015-02-17

    申请号：US12444567

    申请日：2007-10-16

    申请人： In Young Choi ,  Chang Hwan Kim ,  Hyun Ji Lee ,  Seong Hee Park ,  Se Chang Kwon ,  Gwan Sun Lee

    发明人： In Young Choi ,  Chang Hwan Kim ,  Hyun Ji Lee ,  Seong Hee Park ,  Se Chang Kwon ,  Gwan Sun Lee

    IPC分类号： C07H21/04 ,  C12N15/63 ,  C12N9/06 ,  C12N15/85

    CPC分类号： C12N9/003 ,  C12N15/85 ,  C12N2510/00

    摘要： Disclosed herein is an inducible high-expression cassette comprising a dihydrofolate reductase (DHFR) promoter from which GC-rich repeat sequences are partially or entirely removed, the cassette capable of more effectively improving a gene amplification system. Also disclosed are an expression vector comprising the inducible expression cassette and optionally a gene encoding a recombinant protein of interest, an animal cell line transformed with the expression vector, and a method of mass producing and purifying a recombinant protein by culturing the transformant. The present invention enables the shortening of the time required to establish a cell line producing a recombinant protein of interest at high levels using a low concentration of a DHFR inhibitor, thereby allowing more effective production of the recombinant protein.

    摘要翻译： 本文公开了一种可诱导的高表达盒，其包含二氢叶酸还原酶（DHFR）启动子，从其中部分或全部除去富含GC的重复序列，所述盒能够更有效地改善基因扩增系统。 还公开了包含可诱导表达盒和任选的编码目标重组蛋白的基因，用表达载体转化的动物细胞系的表达载体，以及通过培养转化体大量生产和纯化重组蛋白的方法。 本发明能够缩短使用低浓度的DHFR抑制剂以高水平建立产生重组蛋白质的细胞系所需的时间，从而允许更有效地产生重组蛋白质。

    METHOD FOR MASS PRODUCTION OF FACTOR VII/VIIA
    8.
    发明申请
    METHOD FOR MASS PRODUCTION OF FACTOR VII/VIIA 有权
    大量生产VII / VIIA因子的方法

    公开(公告)号：US20130273634A1

    公开(公告)日：2013-10-17

    申请号：US13881974

    申请日：2011-10-26

    申请人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Sung Hwan Hong ,  Se Chang Kwon

    发明人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Sung Hwan Hong ,  Se Chang Kwon

    IPC分类号： C12N9/64

    CPC分类号： C12N9/6437 ,  C07K14/745 ,  C12N15/85 ,  C12N2830/85

    摘要： A method for the mass production of human coagulation factor VII. The method includes a) providing an expression vector carrying i) a dihydrofolate reductase promoter devoid of one or more CCGCC repeat sequences from the GC-rich region thereof and a dihydrofolate reductase (DHFR) gene operably linked thereto and ii) a cytomegalovirus (CMV) promoter and a human coagulation factor VII gene operably linked thereto; b) obtaining a transformed a host cell line containing the expression vector; and c) culturing the transfected host cell in the presence of a dihydrofolate reductase inhibitor to select cells which express human coagulation factor VII with high efficiency; and d) adding sodium butyrate to the selected host cells.

    摘要翻译： 大量生产人凝血因子VII的方法。 所述方法包括：a）提供表达载体，其携带i）没有来自其富含GC的区域的一个或多个CCGCC重复序列的二氢叶酸还原酶启动子和与其可操作地连接的二氢叶酸还原酶（DHFR）基因，和ii）巨细胞病毒（CMV） 启动子和与之有效连接的人凝血因子VII基因; b）获得含有表达载体的转化的宿主细胞系; 和c）在二氢叶酸还原酶抑制剂存在下培养转染的宿主细胞以选择以高效率表达人凝血因子VII的细胞; 和d）向所选择的宿主细胞中加入丁酸钠。

    Method for mass production of factor VII/VIIA
    10.
    发明授权
    Method for mass production of factor VII/VIIA 有权
    大量生产因子VII / VIIA的方法

    公开(公告)号：US09243237B2

    公开(公告)日：2016-01-26

    申请号：US13881974

    申请日：2011-10-26

    申请人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Sung Hwan Hong ,  Se Chang Kwon

    发明人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Sung Hwan Hong ,  Se Chang Kwon

    IPC分类号： A61K38/00 ,  C12N9/64 ,  C07K14/745

    CPC分类号： C12N9/6437 ,  C07K14/745 ,  C12N15/85 ,  C12N2830/85

    摘要： A method for the mass production of human coagulation Factor VII. The method includes a) providing an expression vector carrying i) a dihydrofolate reductase promoter devoid of one or more CCGCC repeat sequences from the GC-rich region thereof and a dihydrofolate reductase (DHFR) gene operably linked thereto and ii) a cytomegalovirus (CMV) promoter and a human coagulation Factor VII gene operably linked thereto; b) obtaining a transformed host cell line containing the expression vector; and c) culturing the transfected host cell in the presence of a dihydrofolate reductase inhibitor to select cells which express human coagulation Factor VII with high efficiency; and d) adding sodium butyrate to the selected host cells.

    摘要翻译： 大量生产人凝血因子VII的方法。 所述方法包括：a）提供表达载体，其携带i）没有来自其富含GC的区域的一个或多个CCGCC重复序列的二氢叶酸还原酶启动子和与其可操作地连接的二氢叶酸还原酶（DHFR）基因，和ii）巨细胞病毒（CMV） 启动子和与之有效连接的人凝血因子VII基因; b）获得含有表达载体的转化的宿主细胞系; 和c）在二氢叶酸还原酶抑制剂存在下培养转染的宿主细胞以选择以高效率表达人凝血因子VII的细胞; 和d）向所选择的宿主细胞中加入丁酸钠。