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公开(公告)号：US12123033B2
公开(公告)日：2024-10-22
申请号：US17079097
申请日：2020-10-23
发明人： Jessica Woodley , Bernice Thommandru , Joseph Dobosy , Mark Behlke , Adam Clore , Garrett Rettig , Beimeng Sun
IPC分类号： C12N15/66 , C12N9/22 , C12N15/113
CPC分类号： C12N9/22 , C12N15/113 , C12N15/66 , C12N2310/122 , C12N2310/20 , C12N2310/321 , C12N2310/531 , C12N2800/80
摘要： Described herein are compositions and methods for improving homology directed repair (HDR) efficiency and reducing homology-independent integration following introduction of double strand breaks with engineered nucleases. Additionally, modifications to double stranded DNA donors to improve the donor potency and efficiency of homology directed repair following introduction of double stranded breaks with programmable nucleases.
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公开(公告)号：US20210123035A1
公开(公告)日：2021-04-29
申请号：US17079097
申请日：2020-10-23
发明人： Jessica Woodley , Bernice Thommandru , Joseph Dobosy , Mark Behlke , Adam Clore , Garrett Rettig , Beimeng Sun
IPC分类号： C12N9/22 , C12N15/113
摘要： Described herein are compositions and methods for improving homology directed repair (HDR) efficiency and reducing homology-independent integration following introduction of double strand breaks with engineered nucleases. Additionally, modifications to double stranded DNA donors to improve the donor potency and efficiency of homology directed repair following introduction of double stranded breaks with programmable nucleases.
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公开(公告)号：US12037615B2
公开(公告)日：2024-07-16
申请号：US17079097
申请日：2020-10-23
发明人： Jessica Woodley , Bernice Thommandru , Joseph Dobosy , Mark Behlke , Adam Clore , Garrett Rettig , Beimeng Sun
IPC分类号： C12N15/66 , C12N9/22 , C12N15/113
CPC分类号： C12N9/22 , C12N15/113 , C12N15/66 , C12N2310/122 , C12N2310/20 , C12N2310/321 , C12N2310/531 , C12N2800/80
摘要： Described herein are compositions and methods for improving homology directed repair (HDR) efficiency and reducing homology-independent integration following introduction of double strand breaks with engineered nucleases. Additionally, modifications to double stranded DNA donors to improve the donor potency and efficiency of homology directed repair following introduction of double stranded breaks with programmable nucleases.
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公开(公告)号：US10982273B2
公开(公告)日：2021-04-20
申请号：US15672685
申请日：2017-08-09
发明人： Joseph Dobosy , Sarah Jacobi , Richard Owczarzy , Mark Behlke , Jessica Lister
IPC分类号： C12Q1/686 , C12N9/22 , C12Q1/6853 , C12Q1/6858 , C12Q1/6862 , C12N15/52
摘要： The invention is directed to methods and kits for performing an RNase H2-mediated cleavage reaction on a sample in an emulsion.
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公开(公告)号：US20240309350A1
公开(公告)日：2024-09-19
申请号：US18679324
申请日：2024-05-30
发明人： Jessica Woodley , Bernice Thommandru , Joseph Dobosy , Mark Behlke , Adam Clore , Garrett Rettig , Beimeng Sun
IPC分类号： C12N9/22 , C12N15/113 , C12N15/66
CPC分类号： C12N9/22 , C12N15/113 , C12N15/66 , C12N2310/122 , C12N2310/20 , C12N2310/321 , C12N2310/531 , C12N2800/80
摘要： Described herein are compositions and methods for improving homology directed repair (HDR) efficiency and reducing homology-independent integration following introduction of double strand breaks with engineered nucleases. Additionally, modifications to double stranded DNA donors to improve the donor potency and efficiency of homology directed repair following introduction of double stranded breaks with programmable nucleases.
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公开(公告)号：US11999994B2
公开(公告)日：2024-06-04
申请号：US17574842
申请日：2022-01-13
发明人： Joseph Dobosy , Scott D. Rose , Jeffrey A. Manthey , Shawn D. Allen , Steven A. Henck , Mark Behlke
IPC分类号： C07H21/04 , C12N15/113 , C12Q1/6837 , C12Q1/6876
CPC分类号： C12Q1/6837 , C12N15/113 , C12Q1/6876 , C12N2310/11 , C12N2310/3519 , C12N2320/11
摘要： Described herein are compositions and methods for the production and quantification of barcoded or unique molecular identifier (UMI)-labeled substrates. In one aspect, the substrate is a bead comprising a template oligonucleotide that is elongated by successive extension reactions to provide a bead with an oligonucleotide comprising a plurality of barcodes and conserved anchor regions. Methods are also described for quantifying the amount of template oligonucleotide loaded onto the substrate and the products of the extension reaction after each round and after the final extension.
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7.发明申请公开(公告)号：US20180142241A1
公开(公告)日：2018-05-24
申请号：US15849887
申请日：2017-12-21
发明人： John J. Rossi , Mark Behlke , Dongho Kim
IPC分类号： C12N15/113 , C12N15/11
CPC分类号： C12N15/113 , C12N15/111 , C12N2310/14 , C12N2310/33 , C12N2310/53 , C12N2310/533 , C12N2320/35 , C12N2320/51
摘要： The invention is directed to compositions and methods for selectively reducing the expression of a gene product from a desired target gene in a cell, as well as for treating diseases caused by the expression of the gene. More particularly, the invention is directed to compositions that contain double stranded RNA (“dsRNA”), and methods for preparing them, that are capable of reducing the expression of target genes in eukaryotic cells. The dsRNA has a first oligonucleotide sequence that is between 25 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of at least 19 nucleotides is sufficiently complementary to a nucleotide sequence of the RNA produced from the target gene to trigger the destruction of the target RNA by the RNAi machinery.
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公开(公告)号：US20180044716A1
公开(公告)日：2018-02-15
申请号：US15672685
申请日：2017-08-09
发明人： Joseph Dobosy , Sarah Jacobi , Richard Owczarzy , Mark Behlke
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12N9/22 , C12N15/52 , C12Q1/6853 , C12Q1/6858 , C12Q1/6862 , C12Y301/26004 , C12Q2521/327 , C12Q2563/159
摘要： The invention is directed to methods and kits for performing an RNase H2-mediated cleavage reaction on a sample in an emulsion.
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公开(公告)号：US20170218438A1
公开(公告)日：2017-08-03
申请号：US15421577
申请日：2017-02-01
发明人： Joseph Dobosy , Aurita Menezes , Caifu Chen , Mark Behlke
CPC分类号： C12Q1/6853 , C12N9/1252 , C12N9/22 , C12P19/34 , C12Q1/6848 , C12Q1/6858 , C12Y207/07007 , C12Y301/26004 , C12Q2521/327 , C12Q2525/121 , C12Q2525/131 , C12Q2525/186 , C12Q2531/119 , C12Q2549/101
摘要： The invention provides a more efficient and less error-prone method of performing LAMP. The invention also provides a method for utilizing an RNase H2-cleavable probe as a technique for generating signal from the reaction, potentially increasing the specificity of the signal generation.
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