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公开(公告)号：US12123033B2
公开(公告)日：2024-10-22
申请号：US17079097
申请日：2020-10-23
发明人： Jessica Woodley , Bernice Thommandru , Joseph Dobosy , Mark Behlke , Adam Clore , Garrett Rettig , Beimeng Sun
IPC分类号： C12N15/66 , C12N9/22 , C12N15/113
CPC分类号： C12N9/22 , C12N15/113 , C12N15/66 , C12N2310/122 , C12N2310/20 , C12N2310/321 , C12N2310/531 , C12N2800/80
摘要： Described herein are compositions and methods for improving homology directed repair (HDR) efficiency and reducing homology-independent integration following introduction of double strand breaks with engineered nucleases. Additionally, modifications to double stranded DNA donors to improve the donor potency and efficiency of homology directed repair following introduction of double stranded breaks with programmable nucleases.
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公开(公告)号：US12024742B2
公开(公告)日：2024-07-02
申请号：US17558994
申请日：2021-12-22
发明人： Joseph Dobosy , John Froehlig, Jr. , Katherine Perschbacher , Kristin Beltz , Scott Rose , Mark Aaron Behlke
IPC分类号： C12Q1/6848 , C12N9/22 , C12Q1/6853 , C12Q1/686
CPC分类号： C12Q1/6848 , C12N9/22 , C12Y301/26004 , C12Q1/6853 , C12Q1/686
摘要： The present invention pertains to hybrid RNase H2 proteins that include fragments of amino acid sequences from Pyrococcus abyssi (P.a.), Thermococcus kodakarensis (T.kod), and Pyrococcus furiosus organisms, as well as methods of using the same to improve mismatch discrimination and activity in a high-fidelity DNA polymerase buffer.
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公开(公告)号：US20210123035A1
公开(公告)日：2021-04-29
申请号：US17079097
申请日：2020-10-23
发明人： Jessica Woodley , Bernice Thommandru , Joseph Dobosy , Mark Behlke , Adam Clore , Garrett Rettig , Beimeng Sun
IPC分类号： C12N9/22 , C12N15/113
摘要： Described herein are compositions and methods for improving homology directed repair (HDR) efficiency and reducing homology-independent integration following introduction of double strand breaks with engineered nucleases. Additionally, modifications to double stranded DNA donors to improve the donor potency and efficiency of homology directed repair following introduction of double stranded breaks with programmable nucleases.
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公开(公告)号：US20180057868A1
公开(公告)日：2018-03-01
申请号：US15689084
申请日：2017-08-29
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12N2310/122 , C12Q1/6806 , C12Q1/6809 , C12Q1/6858 , C12Q1/6869 , C12Q2521/327 , C12Q2525/121 , C12Q2525/161 , C12Q2525/186 , C12Q2525/301 , C12Q2531/113
摘要： The invention describes composition and methods of use for novel hairpin blocked-cleavable primers. In one embodiment unblocking occurs through action of RNase H2. The method improves the specificity of PCR and reduces primer dimer events, enabling higher level multiplex reactions. Additionally, the invention protects RNA-containing primers from attack by single-strand RNases.
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公开(公告)号：US20240318237A1
公开(公告)日：2024-09-26
申请号：US18667876
申请日：2024-05-17
发明人： Joseph Dobosy , John Froehlig, JR. , Katherine Perschbacher , Kristin Beltz , Scott Rose , Mark Aaron Behlke
IPC分类号： C12Q1/6848 , C12N9/22 , C12Q1/6853 , C12Q1/686
CPC分类号： C12Q1/6848 , C12N9/22 , C12Y301/26004 , C12Q1/6853 , C12Q1/686
摘要： The present invention pertains to a kit for producing an extended primer, comprising at least one container providing a hybrid RNase H2 protein, including a hybrid RNase H2 protein comprising fragments of amino acid sequences from Pyrococcus abyssi (P. a.), Thermococcus kodakarensis (T. kod), and Pyrococcus furiosus organisms; a hybrid RNase H2 protein comprises amino acid residues 26-40 and residues 100-120 of T. kod RNase H2; a hybrid RNase H2 protein is selected from SEQ ID NO: 2 and 3; and a hybrid RNase H2 protein is selected from SEQ ID NO: 14-20.
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公开(公告)号：US20220348997A1
公开(公告)日：2022-11-03
申请号：US17558994
申请日：2021-12-22
发明人： Joseph Dobosy , John Froehlig, Jr. , Katherine Perschbacher , Kristin Beltz , Scott Rose , Mark Aaron Behlke
IPC分类号： C12Q1/6848 , C12N9/22
摘要： The present invention pertains to hybrid RNase H2 proteins that include fragments of amino acid sequences from Pyrococcus abyssi (P.a.), Thermococcus kodakarensis (T.kod), and Pyrococcus furiosus organisms, as well as methods of using the same to improve mismatch discrimination and activity in a high-fidelity DNA polymerase buffer.
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公开(公告)号：US10858699B2
公开(公告)日：2020-12-08
申请号：US15689084
申请日：2017-08-29
IPC分类号： C12P19/34 , C12Q1/6853 , C12Q1/6858 , C12Q1/6806 , C12Q1/6809 , C12Q1/6869
摘要： The invention describes composition and methods of use for novel hairpin blocked-cleavable primers. In one embodiment unblocking occurs through action of RNase H2. The method improves the specificity of PCR and reduces primer dimer events, enabling higher level multiplex reactions. Additionally, the invention protects RNA-containing primers from attack by single-strand RNases.
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公开(公告)号：US20190221290A1
公开(公告)日：2019-07-18
申请号：US16374751
申请日：2019-04-04
IPC分类号： G16B30/00 , C12Q1/6858
CPC分类号： G16B30/00 , C12Q1/6858 , C12Y301/26004 , C12Q2525/121 , C12Q2525/131 , C12Q2525/186
摘要： The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays. The invention also provides methods for detection of DNA sequences altered after cleavage by a targetable endonuclease, such as the CRISPR Cas9 protein from the bacterium Streptococcus pyogenes.
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公开(公告)号：US12037615B2
公开(公告)日：2024-07-16
申请号：US17079097
申请日：2020-10-23
发明人： Jessica Woodley , Bernice Thommandru , Joseph Dobosy , Mark Behlke , Adam Clore , Garrett Rettig , Beimeng Sun
IPC分类号： C12N15/66 , C12N9/22 , C12N15/113
CPC分类号： C12N9/22 , C12N15/113 , C12N15/66 , C12N2310/122 , C12N2310/20 , C12N2310/321 , C12N2310/531 , C12N2800/80
摘要： Described herein are compositions and methods for improving homology directed repair (HDR) efficiency and reducing homology-independent integration following introduction of double strand breaks with engineered nucleases. Additionally, modifications to double stranded DNA donors to improve the donor potency and efficiency of homology directed repair following introduction of double stranded breaks with programmable nucleases.
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公开(公告)号：US20210395799A1
公开(公告)日：2021-12-23
申请号：US17226044
申请日：2021-04-08
IPC分类号： C12Q1/6827 , C12Q1/6858 , G16B30/00 , G16B20/20 , C12N9/22 , C12N15/11 , C12Q1/6853 , C12Q1/6876
摘要： The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays. The invention also provides methods for detection of DNA sequences altered after cleavage by a targetable endonuclease, such as the CRISPR Cas9 protein from the bacterium Streptococcus pyogenes.
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