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公开(公告)号：US11940413B2
公开(公告)日：2024-03-26
申请号：US17194850
申请日：2021-03-08
发明人： Steven Gordon , Thomas Hagerott , Edmund Golaski , Jerzy Olejnik
IPC分类号： C12Q1/68 , B01L3/00 , C12Q1/6869 , G01N27/447
CPC分类号： G01N27/44791 , B01L3/502761 , C12Q1/6869 , G01N27/44726 , B01L3/502715 , B01L2200/0668 , B01L2300/0877 , B01L2400/0487
摘要： The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.
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公开(公告)号：US11866741B2
公开(公告)日：2024-01-09
申请号：US17350078
申请日：2021-06-17
发明人： Jerzy Olejnik , Angela Delucia
IPC分类号： C12N9/12 , C12Q1/6848 , C12Q1/6844 , C12Q1/6869
CPC分类号： C12N9/1252 , C12Q1/6844 , C12Q1/6869 , C12Y207/07007
摘要： The present invention relates to a polymerase enzyme from 9° N with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising mutations in the motif A region. The invention also relates to methods of using such enzymes as well as a kit with such polymerases.
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公开(公告)号：US11548909B2
公开(公告)日：2023-01-10
申请号：US15928562
申请日：2018-03-22
发明人： Mong Sano Marma , Jerzy Olejnik , Ilia Korboukh
IPC分类号： C12P19/34 , C07H19/10 , C07F7/18 , C07C323/12 , C09B11/24 , C09B23/08 , C07H19/14 , C09B1/00 , C09B5/24 , C09B23/16 , C12Q1/6869 , C12Q1/6876 , C07C323/60 , C07D207/46 , C12Q1/6811
摘要： The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
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公开(公告)号：US20220162251A1
公开(公告)日：2022-05-26
申请号：US17360427
申请日：2021-06-28
发明人： Mong Sano MARMA , Jerzy Olejnik , Ilia Korboukh
IPC分类号： C07H19/10 , C07F7/18 , C07C323/12 , C09B11/24 , C09B23/08 , C07H19/14 , C09B1/00 , C09B5/24 , C09B23/16 , C12Q1/6869 , C12Q1/6876 , C07C323/60 , C07D207/46 , C12Q1/6811
摘要： The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
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公开(公告)号：US20220127587A1
公开(公告)日：2022-04-28
申请号：US16485281
申请日：2018-02-13
发明人： Jerzy Olejnik , Angela Delucia , Cheng-yao Chen , Ryan Charles Heller , Thomas William Schoenfeld
IPC分类号： C12N9/12 , C12N15/63 , C12Q1/6869
摘要： The present invention relates to a polymerase enzyme from Pyrococcus furiosus with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising the following mutations in the motif A region (SGS). It relates to a polymerase enzyme according to SEQ ID NO. 1 or any polymerase that shares at least 70% amino acid sequence identity thereto, comprising a mutation selected from the group of (i) at position 409 of SEQ ID NO. 3: serine (S) (L409S) and/or, (ii) at position 410 of SEQ ID NO. 3: glycine (G) (Y410G) and/or (iii) at position 411 of SEQ ID NO. 3: serine (S) (P411S), wherein the enzyme has little or no 3′-5′ exonuclease activity.
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公开(公告)号：US11117921B2
公开(公告)日：2021-09-14
申请号：US16423466
申请日：2019-05-28
发明人： Mong Sano Marma , Jerzy Olejnik , Ilia Korboukh
IPC分类号： C07H19/10 , C07H19/14 , C07F7/18 , C07C323/12 , C09B11/24 , C09B23/08 , C09B1/00 , C09B5/24 , C09B23/16 , C12Q1/6869 , C12Q1/6876 , C07C323/60 , C07D207/46 , C12Q1/6811
摘要： The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
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公开(公告)号：US20220145272A1
公开(公告)日：2022-05-12
申请号：US16485282
申请日：2018-02-13
发明人： Jerzy Olejnik , Angela Delucia , Cheng-yao Chen , Ryan Charles Heller , Thomas William Schoenfeld
IPC分类号： C12N9/12
摘要： The present invention relates to a polymerase enzyme with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising the following mutations in the motif A region (SGS). It relates to a polymerase enzyme according to SEQ ID NO. 1 with mutations in the amino acid sequence positions 409, 410 and 411.
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公开(公告)号：US11078526B2
公开(公告)日：2021-08-03
申请号：US16422056
申请日：2019-05-24
IPC分类号： C12Q1/6823 , C12Q1/6869
摘要： The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis methods. In particular, the present invention contemplates the use of polyphenolic compounds, known as antioxidant additives, to improve the efficiency of Sequencing-By-Synthesis reactions. For example, gallic acid (GA) is shown herein to be one of many exemplary SBS polyphenolic additives.
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