公开(公告)号：US11548909B2

公开(公告)日：2023-01-10

申请号：US15928562

申请日：2018-03-22

申请人： IsoPlexis Corporation

发明人： Mong Sano Marma ,  Jerzy Olejnik ,  Ilia Korboukh

IPC分类号： C12P19/34 ,  C07H19/10 ,  C07F7/18 ,  C07C323/12 ,  C09B11/24 ,  C09B23/08 ,  C07H19/14 ,  C09B1/00 ,  C09B5/24 ,  C09B23/16 ,  C12Q1/6869 ,  C12Q1/6876 ,  C07C323/60 ,  C07D207/46 ,  C12Q1/6811

摘要： The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

公开(公告)号：US11117921B2

公开(公告)日：2021-09-14

申请号：US16423466

申请日：2019-05-28

申请人： IsoPlexis Corporation

发明人： Mong Sano Marma ,  Jerzy Olejnik ,  Ilia Korboukh

IPC分类号： C07H19/10 ,  C07H19/14 ,  C07F7/18 ,  C07C323/12 ,  C09B11/24 ,  C09B23/08 ,  C09B1/00 ,  C09B5/24 ,  C09B23/16 ,  C12Q1/6869 ,  C12Q1/6876 ,  C07C323/60 ,  C07D207/46 ,  C12Q1/6811

摘要： The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

公开(公告)号：US11821032B2

公开(公告)日：2023-11-21

申请号：US16999329

申请日：2020-08-21

申请人： IsoPlexis Corporation

发明人： Luisa Andruzzi ,  Jimmy Adediran ,  Timothy Pelletier ,  Austin Ricker ,  Angela DeLucia ,  Ben McNally ,  Dona Hevroni ,  Mong Sano Marma ,  John Andrew Sheridan

IPC分类号： C12P19/34 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6806 ,  C07D275/03

CPC分类号： C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6876 ,  C07D275/03 ,  C12Q2525/113 ,  C12Q2525/186 ,  C12Q2600/16 ,  C12Q1/6869 ,  C12Q2525/113 ,  C12Q2525/117 ,  C12Q2525/186

摘要： The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3′-O position capped by a disulfide-based 3′-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

公开(公告)号：US11421264B2

公开(公告)日：2022-08-23

申请号：US15457344

申请日：2017-03-13

申请人： IsoPlexis Corporation

发明人： Mong Sano Marma ,  Luisa Andruzzi ,  Ben McNally ,  Angela DeLucia ,  Dona Hevroni ,  Elizabeth Carpenter ,  Magnus Besev

IPC分类号： C12Q1/68 ,  C12Q1/6823 ,  C07D405/14 ,  C07F9/6561 ,  C07D405/04 ,  C07D491/22 ,  C12Q1/6874 ,  C07H19/14 ,  C07H19/10 ,  C07D519/00 ,  C07F9/6558 ,  C07F7/18 ,  C09B23/08 ,  C09B11/24

摘要： The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.