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公开(公告)号:US07955814B2
公开(公告)日:2011-06-07
申请号:US11671953
申请日:2007-02-06
申请人: Arno De Kreij , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe , Mark Turner , Jonathan Goodwins
发明人: Arno De Kreij , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe , Mark Turner , Jonathan Goodwins
CPC分类号: C12Y301/01032 , A23C9/1216 , A23C19/0328 , A23C19/054 , A23L15/25 , A23L23/00 , A23L27/60 , A23L29/06 , A23L29/10 , A23V2002/00 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , A23V2200/222 , A23V2250/192 , A23V2250/1846 , A23V2250/2136 , A23V2250/21372 , A23V2250/21362
摘要: A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an additional emulsifier.
摘要翻译: 一种在食品中原位生产乳化剂的方法,其中将脂质酰基转移酶加入食品中。 优选地,在不增加或不显着增加食品的游离脂肪酸含量的情况下生产乳化剂。 优选地,脂质酰基转移酶是能够将酰基从脂质转移到以下酰基受体中的一种或多种:甾醇,甾烷醇,碳水化合物,蛋白质或其亚单位的甘油。 优选地,除了乳化剂之外,可以制备一种或多种甾烷醇酯或甾烷醇酯或蛋白酯或碳水化合物酯或甘油二酯或甘油单酯。 这些中的一种或多种可以用作另外的乳化剂。
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公开(公告)号:US20100285525A1
公开(公告)日:2010-11-11
申请号:US12400268
申请日:2009-03-09
CPC分类号: A21D8/042 , A23C9/1216 , A23C19/0328 , A23C19/054 , A23D7/013 , A23G9/363 , A23L15/25 , A23L23/00 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , A23V2002/00 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918 , A23V2200/222 , A23V2250/192 , A23V2250/1846 , A23V2250/2136 , A23V2250/21372 , A23V2250/21362
摘要: A method of producing one or more of a carbohydrate ester, a protein ester, a protein subunit ester or a hydroxyl acid ester, which method comprises admixing an acyl donor, an acyl acceptor and water to produce a high water environment comprising 5-98% water, wherein said acyl donor is a lipid substrate selected from one or more of the group consisting of a phospholipid, a lysophospholipid, a triacylglyceride, a diglyceride, a glycolipid or a lysoglycolipid and said acyl acceptor is selected from one ore more of the group consisting of a carbohydrate, a protein, a protein subunit, or a hydroxyl acid; and contacting the admixture with a lipid acyltransferase, such that said lipid acyl transferase catalyses one or both of the following reactions: alcoholysis or transesterification.
摘要翻译: 制备碳水化合物酯,蛋白质酯,蛋白质亚基酯或羟基酸酯中的一种或多种的方法,该方法包括将酰基供体,酰基受体和水混合以产生高水环境,其包含5-98% 水,其中所述酰基供体是选自磷脂,溶血磷脂,三酰基甘油酯,甘油二酯,糖脂或溶血糖脂中的一种或多种的脂质底物,并且所述酰基受体选自一组或多于一种 由碳水化合物,蛋白质,蛋白质亚基或羟基酸组成; 并将该混合物与脂质酰基转移酶接触,使得所述脂质酰基转移酶催化下列一个或两个反应:醇解或酯交换反应。
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公开(公告)号:US08003095B2
公开(公告)日:2011-08-23
申请号:US11483331
申请日:2006-07-07
CPC分类号: A21D8/042 , A23C9/1216 , A23C19/0328 , A23C19/054 , A23D7/013 , A23G9/363 , A23L15/25 , A23L23/00 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , A23V2002/00 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918 , A23V2200/222 , A23V2250/192 , A23V2250/1846 , A23V2250/2136 , A23V2250/21372 , A23V2250/21362
摘要: A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an additional emulsifier.
摘要翻译: 一种在食品中原位生产乳化剂的方法,其中将脂质酰基转移酶加入食品中。 优选地,在不增加或不显着增加食品的游离脂肪酸含量的情况下生产乳化剂。 优选地,脂质酰基转移酶是能够将酰基从脂质转移到以下酰基受体中的一种或多种:甾醇,甾烷醇,碳水化合物,蛋白质或其亚单位的甘油。 优选地,除了乳化剂之外,可以制备一种或多种甾烷醇酯或甾烷醇酯或蛋白酯或碳水化合物酯或甘油二酯或甘油单酯。 这些中的一种或多种可以用作另外的乳化剂。
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公开(公告)号:US07807398B2
公开(公告)日:2010-10-05
申请号:US11182408
申请日:2005-07-15
CPC分类号: A21D8/042 , A23C9/1216 , A23C19/0328 , A23C19/054 , A23D7/013 , A23G9/363 , A23L15/25 , A23L23/00 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , A23V2002/00 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918 , A23V2200/222 , A23V2250/192 , A23V2250/1846 , A23V2250/2136 , A23V2250/21372 , A23V2250/21362
摘要: A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an addition emulsifier.
摘要翻译: 一种在食品中原位生产乳化剂的方法,其中将脂质酰基转移酶加入食品中。 优选地,在不增加或不显着增加食品的游离脂肪酸含量的情况下生产乳化剂。 优选地,脂质酰基转移酶是能够将酰基从脂质转移到以下酰基受体中的一种或多种:甾醇,甾烷醇,碳水化合物,蛋白质或其亚单位的甘油。 优选地,除了乳化剂之外,可以制备一种或多种甾烷醇酯或甾烷醇酯或蛋白酯或碳水化合物酯或甘油二酯或甘油单酯。 这些中的一种或多种可以用作加成乳化剂。
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公开(公告)号:US08278062B2
公开(公告)日:2012-10-02
申请号:US12400268
申请日:2009-03-09
CPC分类号: A21D8/042 , A23C9/1216 , A23C19/0328 , A23C19/054 , A23D7/013 , A23G9/363 , A23L15/25 , A23L23/00 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , A23V2002/00 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918 , A23V2200/222 , A23V2250/192 , A23V2250/1846 , A23V2250/2136 , A23V2250/21372 , A23V2250/21362
摘要: A method of producing one or more of a carbohydrate ester, a protein ester, a protein subunit ester or a hydroxyl acid ester, which method comprises admixing an acyl donor, an acyl acceptor and water to produce a high water environment comprising 5-98% water, wherein said acyl donor is a lipid substrate selected from one or more of the group consisting of a phospholipid, a lysophospholipid, a triacylglyceride, a diglyceride, a glycolipid or a lysoglycolipid and said acyl acceptor is selected from one ore more of the group consisting of a carbohydrate, a protein, a protein subunit, or a hydroxyl acid; and contacting the admixture with a lipid acyltransferase, such that said lipid acyl transferase catalyses one or both of the following reactions: alcoholysis or transesterification.
摘要翻译: 制备碳水化合物酯,蛋白质酯,蛋白质亚基酯或羟基酸酯中的一种或多种的方法,该方法包括将酰基供体,酰基受体和水混合以产生高水环境,其包含5-98% 水,其中所述酰基供体是选自磷脂,溶血磷脂,三酰基甘油酯,甘油二酯,糖脂或溶血糖脂中的一种或多种的脂质底物,并且所述酰基受体选自一组或多于一种 由碳水化合物,蛋白质,蛋白质亚基或羟基酸组成; 并将该混合物与脂质酰基转移酶接触,使得所述脂质酰基转移酶催化下列一个或两个反应:醇解或酯交换反应。
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公开(公告)号:US07955813B2
公开(公告)日:2011-06-07
申请号:US11483345
申请日:2006-07-07
CPC分类号: A21D8/042 , A23C9/1216 , A23C19/0328 , A23C19/054 , A23D7/013 , A23G9/363 , A23L15/25 , A23L23/00 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , A23V2002/00 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918 , A23V2200/222 , A23V2250/192 , A23V2250/1846 , A23V2250/2136 , A23V2250/21372 , A23V2250/21362
摘要: A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an additional emulsifier.
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公开(公告)号:US07638293B2
公开(公告)日:2009-12-29
申请号:US11182480
申请日:2005-07-15
CPC分类号: A21D8/042 , A23C9/1216 , A23C19/0328 , A23C19/054 , A23D7/013 , A23G9/363 , A23L15/25 , A23L23/00 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , A23V2002/00 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918 , A23V2200/222 , A23V2250/192 , A23V2250/1846 , A23V2250/2136 , A23V2250/21372 , A23V2250/21362
摘要: Provided is a method of producing one or more of a carbohydrate ester, a protein ester, a protein subunit ester or a hydroxyl acid ester. The method comprises admixing an acyl donor, an acyl acceptor and water to produce a high water environment comprising 5-98% water. Preferably, the acyl donor is a lipid substrate selected from one or more of the group consisting of a phospholipid, a lysophospholipid, a triacylglyceride, a diglyceride, a glycolipid or a lysoglycolipid. Preferably, the acyl acceptor is selected from one ore more of the group consisting of a carbohydrate, a protein, a protein subunit, or a hydroxyl acid. The method further comprises contacting the admixture with a lipid acyltransferase, such that said lipid acyl transferase catalyses alcoholysis and/or transesterification.
摘要翻译: 提供一种生产碳水化合物酯,蛋白质酯,蛋白质亚基酯或羟基酸酯中的一种或多种的方法。 该方法包括将酰基供体,酰基受体和水混合以产生包含5-98%水的高水环境。 优选地,酰基供体是选自磷脂,溶血磷脂,三酰基甘油酯,甘油二酯,糖脂或溶血糖脂中的一种或多种的脂质底物。 优选地,酰基受体选自碳水化合物,蛋白质,蛋白质亚基或羟基酸中的一个以上的一个。 所述方法还包括使所述混合物与脂质酰基转移酶接触,使得所述脂质酰基转移酶催化醇解和/或酯交换。
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公开(公告)号:US08440435B2
公开(公告)日:2013-05-14
申请号:US12492042
申请日:2009-06-25
CPC分类号: C11C1/08 , C11C3/06 , C12N9/1029 , C12P7/6436 , C12P7/6445 , C12Y203/01
摘要: The present application provides a method of reducing and/or removing diglyceride from an edible oil, comprising admixing an edible oil with an acyl acceptor substrate and a diglyceride:glycerol acyltransferase, wherein the diglyceride:glycerol acyltransferase is characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol. The diglyceride:glycerol acyltransferase can comprise the amino acid sequence motif GDSX. The present invention also relates to the use of a diglyceride:glycerol acyltransferase in the manufacture of an edible oil, for reducing and/or removing diglyceride from said edible oil and to the use of said enzyme in the manufacture of a foodstuff comprising an edible oil for improving the crystallization properties of said foodstuff.
摘要翻译: 本申请提供了从食用油中还原和/或除去甘油二酯的方法,包括将食用油与酰基受体底物和甘油二酯:甘油酰基转移酶混合,其中所述甘油二酯:甘油酰基转移酶被表征为可食用的酶 油能够将酰基从甘油二酯转移到甘油。 甘油二酯:甘油酰基转移酶可以包含氨基酸序列基序GDSX。 本发明还涉及甘油二酯:甘油酰基转移酶在制造食用油中的用途,用于从所述食用油中还原和/或除去甘油二酯,以及所述酶在制造包含食用油的食品中的用途 用于改善所述食品的结晶性能。
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公开(公告)号:US20100086640A1
公开(公告)日:2010-04-08
申请号:US12552572
申请日:2009-09-02
CPC分类号: A21D8/042 , A23C19/0328 , A23D7/02 , A23K20/189 , A23K50/75 , A23L7/107 , A23L7/109 , A23L7/111 , A23L27/60 , A23L29/06 , C11B3/003 , C12N9/18 , C12P7/62 , C12P19/44 , C12Y301/01
摘要: A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolyzing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.
摘要翻译: 与甘油三酯相比,极性脂质活性比例更高的真菌野生型脂肪分解酶,其中所述酶优选具有至少为4的磷脂:甘油三酯活性比。优选地,根据本发明的脂肪分解酶具有糖脂: 甘油三酯水解活性比至少为1.5。 在一个实施方案中,根据本发明的真菌脂肪分解酶包含如SEQ ID NO:1或SEQ ID No.2或SEQ ID No.4或SEQ ID No.6所示的氨基酸序列或氨基酸序列, 与其具有至少90%的同一性。 本发明还包括编码真菌脂肪分解酶的核酸,该核酸选自:(a)包含SEQ ID No.3,SEQ ID No.5或SEQ ID No.5所示核苷酸的核酸 第7号 (b)通过遗传密码的退化与SEQ ID No.3,SEQ ID No.5或SEQ ID No.7的核苷酸序列有关的核酸; 和(c)核酸,其包含与SEQ ID No.3,SEQ ID No.5或SEQ ID No.7所示的核苷酸序列具有至少90%同一性的核苷酸序列。
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10.
公开(公告)号:US07622290B2
公开(公告)日:2009-11-24
申请号:US11519734
申请日:2006-09-12
CPC分类号: A21D8/042 , A23C19/0328 , A23D7/02 , A23K20/189 , A23K50/75 , A23L7/107 , A23L7/109 , A23L7/111 , A23L27/60 , A23L29/06 , C11B3/003 , C12N9/18 , C12P7/62 , C12P19/44 , C12Y301/01
摘要: A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolyzing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.
摘要翻译: 与甘油三酯相比,极性脂质活性比例更高的真菌野生型脂肪分解酶,其中所述酶优选具有至少为4的磷脂:甘油三酯活性比。优选地,根据本发明的脂肪分解酶具有糖脂: 甘油三酯水解活性比至少为1.5。 在一个实施方案中,根据本发明的真菌脂肪分解酶包含如SEQ ID NO:1或SEQ ID No.2或SEQ ID No.4或SEQ ID No.6所示的氨基酸序列或氨基酸序列, 与其具有至少90%的同一性。 本发明还包括编码真菌脂肪分解酶的核酸,该核酸选自:(a)包含SEQ ID No.3,SEQ ID No.5或SEQ ID No.5所示核苷酸的核酸 第7号 (b)通过遗传密码的退化与SEQ ID No.3,SEQ ID No.5或SEQ ID No.7的核苷酸序列有关的核酸; 和(c)核酸,其包含与SEQ ID No.3,SEQ ID No.5或SEQ ID No.7所示的核苷酸序列具有至少90%同一性的核苷酸序列。
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