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    Synthetic genomes
    1.
    发明申请
    Synthetic genomes 有权
    合成基因组

    公开(公告)号：US20070264688A1

    公开(公告)日：2007-11-15

    申请号：US11635355

    申请日：2006-12-06

    申请人： J. Venter ,  Hamilton Smith ,  Clyde Hutchison

    发明人： J. Venter ,  Hamilton Smith ,  Clyde Hutchison

    IPC分类号： C07H21/04 ,  C12N5/06 ,  C12P1/04

    CPC分类号： C12N15/10 ,  C12N15/1093 ,  C12N15/66

    摘要： Methods are provided for constructing a synthetic genome, comprising generating and assembling nucleic acid cassettes comprising portions of the genome, wherein at least one of the nucleic acid cassettes is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. Rational methods may be used to design the synthetic genome (e.g., to establish a minimal genome and/or to optimize the function of genes within a genome, such as by mutating or rearranging the order of the genes). Synthetic genomes of the invention may be introduced into vesicles (e.g., bacterial cells from which part or all of the resident genome has been removed, or synthetic vesicles) to generate synthetic cells. Synthetic genomes or synthetic cells may be used for a variety of purposes, including the generation of synthetic fuels, such as hydrogen or ethanol.

    摘要翻译： 提供了用于构建合成基因组的方法，包括产生和组装包含基因组部分的核酸盒，其中至少一个核酸盒由化学合成的核酸组分构成，或者由化学合成的 核酸成分。 在一个实施方案中，整个合成基因组由已化学合成的核酸组分或化学合成的核酸组分的拷贝构建。 合理的方法可用于设计合成基因组（例如，建立最小基因组和/或优化基因组内的基因的功能，例如通过突变或重新排列基因的顺序）。 可以将本发明的合成基因组引入囊泡（例如，其中已经除去部分或全部常驻基因组的细菌细胞或合成的囊泡）以产生合成细胞。 合成基因组或合成细胞可以用于各种目的，包括生成合成燃料，例如氢或乙醇。

    Installation of genomes or partial genomes into cells or cell-like systems
    2.
    发明申请
    Installation of genomes or partial genomes into cells or cell-like systems 有权
    将基因组或部分基因组安装到细胞或细胞样系统中

    公开(公告)号：US20070269862A1

    公开(公告)日：2007-11-22

    申请号：US11644713

    申请日：2006-12-22

    申请人： John Glass ,  Lei Young ,  Carole Lartigue ,  Nacyra Assad-Garcia ,  Hamilton Smith ,  Clyde Hutchison ,  J. Venter

    发明人： John Glass ,  Lei Young ,  Carole Lartigue ,  Nacyra Assad-Garcia ,  Hamilton Smith ,  Clyde Hutchison ,  J. Venter

    IPC分类号： C12N15/87 ,  C12N5/06 ,  C12P21/04

    CPC分类号： C12P21/02 ,  C12N2510/00

    摘要： A method is provided for introducing a genome into a cell or cell-like system. The introduced genome may occur in nature, be manmade with or without automation, or may be a hybrid of naturally occurring and manmade materials. The genome is obtained outside of a cell with minimal damage. Materials such as a proteins, RNAs, polycations, nucleoid condensation proteins, or gene translation systems may accompany the genome. The genome is installed into a naturally occurring cell or into a manmade cell-like system. A cell-like system or synthetic cell resulting from the practice of the provided method may be designed and used to yield gene-expression products, such as desired proteins. By enabling the synthesis of cells or cell-like systems comprising a wide variety of genomes, accompanying materials and membrane types, the provided method makes possible a broader field of experimentation and bioengineering than has been available using prior art methods.

    摘要翻译： 提供了将基因组导入细胞或细胞样系统的方法。 引入的基因组可以在自然界中发生，在有或没有自动化的情况下是人造的，或者可以是天然存在的和人造的材料的混合物。 基因组以最小的损伤在细胞外获得。 材料如蛋白质，RNAs，聚阳离子，核仁缩合蛋白或基因翻译系统可能伴随着基因组。 将基因组安装到天然存在的细胞或人造细胞样系统中。 可以设计并使用由所提供的方法的实践产生的细胞样系统或合成细胞来产生基因表达产物，例如所需的蛋白质。 通过实现包含各种各样的基因组，伴随材料和膜类型的细胞或细胞样系统的合成，所提供的方法可能比使用现有技术方法可用的更广泛的实验和生物工程领域。

    Nucleotide sequence of the haemophilus influenzae Rd genome, fragments thereof, and uses thereof
    3.
    发明申请
    Nucleotide sequence of the haemophilus influenzae Rd genome, fragments thereof, and uses thereof 审中-公开
    流感嗜血杆菌Rd基因组的核苷酸序列，其片段及其用途

    公开(公告)号：US20050131222A1

    公开(公告)日：2005-06-16

    申请号：US10981687

    申请日：2004-11-05

    申请人： Robert Fleischmann ,  Mark Adams ,  Owen White ,  Hamilton Smith ,  J. Venter

    发明人： Robert Fleischmann ,  Mark Adams ,  Owen White ,  Hamilton Smith ,  J. Venter

    IPC分类号： C07K14/285 ,  C07H21/04

    CPC分类号： C07K14/285

    摘要： The present invention provides the sequencing of the entire genome of Haemophilus influenzae Rd, SEQ ID NO:1. The present invention further provides the sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use. In addition to the entire genomic sequence, the present invention identifies over 1700 protein encoding fragments of the genome and identifies, by position relative to a unique Not I restriction endonuclease site, any regulatory elements which modulate the expression of the protein encoding fragments of the Haemophilus genome.

    摘要翻译： 本发明提供了流感嗜血杆菌Rd，SEQ ID NO：1的整个基因组的测序。 本发明还提供存储在计算机可读介质上的序列信息，以及便于其使用的基于计算机的系统和方法。 除了整个基因组序列之外，本发明鉴定了基因组的超过1700个蛋白质编码片段，并且通过相对于独特的Not I限制性内切核酸酶位点的位置鉴定调节嗜血杆菌蛋白编码片段的表达的任何调节元件 基因组