公开(公告)号：US08158389B2

公开(公告)日：2012-04-17

申请号：US11545024

申请日：2006-10-10

申请人： James C. Anderson ,  Mervyn L. DeSouza ,  Paula M. Hicks ,  Sherry R. Kollmann

发明人： James C. Anderson ,  Mervyn L. DeSouza ,  Paula M. Hicks ,  Sherry R. Kollmann

IPC分类号： C12P13/22 ,  C12N1/21

CPC分类号： C12P17/10

摘要： Products and methods for the in vivo production of monatin sweetener are provided. The products include microorganisms that are genetically modified to secrete or to improve secretion of monatin; microorganisms that are genetically modified to produce monatin; and microorganisms that are genetically modified to both secrete or improve secretion of monatin and produce monatin. The methods include producing monatin in such genetically engineered microorganisms.

摘要翻译： 提供了莫纳甜甜味剂体内生产的产品和方法。 产品包括经遗传修饰以分泌或改善莫纳甜分泌的微生物; 经遗传修饰以产生莫纳甜的微生物; 和经遗传修饰以分泌或改善莫纳甜分泌并产生莫纳甜的微生物。 这些方法包括在这种基因工程微生物中产生莫纳甜。

公开(公告)号：US08153405B2

公开(公告)日：2012-04-10

申请号：US11379528

申请日：2006-04-20

申请人： James C. Anderson ,  Mervyn L. DeSouza ,  Paula M. Hicks ,  Sherry R. Kollmann ,  Jose Laplaza

发明人： James C. Anderson ,  Mervyn L. DeSouza ,  Paula M. Hicks ,  Sherry R. Kollmann ,  Jose Laplaza

IPC分类号： C12P13/22 ,  C12N1/21

CPC分类号： C12P17/10 ,  A61K31/7048 ,  Y02A50/473

摘要： Products and methods for the in vivo production of monatin sweetener are provided. The products include microorganisms that are genetically modified to secrete or to improve secretion of monatin; microorganisms that are genetically modified to produce monatin; and microorganisms that are genetically modified to both secrete or improve secretion of monatin and produce monatin. The methods include producing monatin in such genetically engineered microorganisms.

公开(公告)号：US20110045547A1

公开(公告)日：2011-02-24

申请号：US12828714

申请日：2010-07-01

申请人： Mervyn L. de Souza ,  Wei Niu ,  Jose Laplaza ,  Christopher Solheid ,  Sherry R. Kollmann ,  Joshua M. Lundorff ,  Paula M. Hicks ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  David P. Weiner ,  Ellen Burke ,  Peter Luginbuhl ,  Analia Bueno ,  Joslin Cuenca

发明人： Mervyn L. de Souza ,  Wei Niu ,  Jose Laplaza ,  Christopher Solheid ,  Sherry R. Kollmann ,  Joshua M. Lundorff ,  Paula M. Hicks ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  David P. Weiner ,  Ellen Burke ,  Peter Luginbuhl ,  Analia Bueno ,  Joslin Cuenca

IPC分类号： C12P13/22 ,  C12P17/10

CPC分类号： C12N9/90 ,  C12P13/06 ,  C12P13/22 ,  C12P17/10

摘要： The invention provides for isomerase (e.g., racemase) and epimerase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such isomerase (e.g., racemase) and epimerase nucleic acids and polypeptides.

摘要翻译： 本发明提供异构酶（例如消旋酶）和差向异构酶多肽以及编码这种多肽的核酸。 还提供了使用这种异构酶（例如消旋酶）和差向异构酶核酸和多肽的方法。

公开(公告)号：US08076108B2

公开(公告)日：2011-12-13

申请号：US11714279

申请日：2007-03-06

申请人： Brian J. Brazeau ,  Mervyn L. De Souza ,  Steven J. Gort ,  Paula M. Hicks ,  Sherry R. Kollmann ,  Jose M. Laplaza ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  Christopher Solheid

发明人： Brian J. Brazeau ,  Mervyn L. De Souza ,  Steven J. Gort ,  Paula M. Hicks ,  Sherry R. Kollmann ,  Jose M. Laplaza ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  Christopher Solheid

IPC分类号： C12P17/10

CPC分类号： C12N9/1096 ,  C12N9/90 ,  C12P17/10

摘要： Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

摘要翻译： 使用多肽和生物合成途径产生莫纳甜和莫纳甜的某些立体异构体，例如R，R莫纳甜和S，R莫纳甜及其盐。 这些多肽和生物合成途径也可用于生产在特定莫纳甜合成途径中形成的包括一些生物合成途径的中间体R-2-羟基-2-（吲哚-3-基甲基）-4-酮戊二酸。

公开(公告)号：US20110020882A1

公开(公告)日：2011-01-27

申请号：US12811491

申请日：2008-12-31

申请人： Mervyn L. de Souza ,  Paula M. Hicks ,  Sherry R. Kollmann ,  Jose Laplaza ,  Joshua M. Lundorff ,  Sara C. McFarlan ,  Erin Marasco ,  Wei Niu ,  Fernando A. Sanchez-Riera ,  Christopher Solheid ,  David P. Weiner ,  Ellen Burke ,  Peter Luginbuhl ,  Analia Bueno ,  Joslin Cuenca

发明人： Mervyn L. de Souza ,  Paula M. Hicks ,  Sherry R. Kollmann ,  Jose Laplaza ,  Joshua M. Lundorff ,  Sara C. McFarlan ,  Erin Marasco ,  Wei Niu ,  Fernando A. Sanchez-Riera ,  Christopher Solheid ,  David P. Weiner ,  Ellen Burke ,  Peter Luginbuhl ,  Analia Bueno ,  Joslin Cuenca

IPC分类号： C12P17/10

CPC分类号： C12P13/227 ,  C12N9/0004 ,  C12N9/1096 ,  C12P17/10

摘要： The invention provides for aminotransferase and oxidoreductase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such aminotransferase and oxidoreductase nucleic acids and polypeptides.

摘要翻译： 本发明提供氨基转移酶和氧化还原酶多肽以及编码这种多肽的核酸。 还提供了使用这种氨基转移酶和氧化还原酶核酸和多肽的方法。

公开(公告)号：US08975046B2

公开(公告)日：2015-03-10

申请号：US13287900

申请日：2011-11-02

申请人： Brian J. Brazeau ,  Mervyn L. De Souza ,  Steven J. Gort ,  Paula M. Hicks ,  Sherry R. Kollmann ,  Jose M. Laplaza ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  Christopher Solheid

发明人： Brian J. Brazeau ,  Mervyn L. De Souza ,  Steven J. Gort ,  Paula M. Hicks ,  Sherry R. Kollmann ,  Jose M. Laplaza ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  Christopher Solheid

IPC分类号： C12P17/10 ,  C12P13/22 ,  C12N9/10 ,  C12N9/90

CPC分类号： C12N9/1096 ,  C12N9/90 ,  C12P17/10

摘要： Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

公开(公告)号：US08785162B2

公开(公告)日：2014-07-22

申请号：US12811491

申请日：2008-12-31

申请人： Mervyn L. De Souza ,  Paula M. Hicks ,  Sherry R. Kollmann ,  Jose Laplaza ,  Joshua M. Lundorff ,  Sara C. McFarlan ,  Erin Marasco ,  Wei Niu ,  Fernando A. Sanchez-Riera ,  Christopher Solheid ,  David P. Weiner ,  Peter Luginbuhl ,  Analia Bueno ,  Joslin Cuenca

发明人： Mervyn L. De Souza ,  Paula M. Hicks ,  Sherry R. Kollmann ,  Jose Laplaza ,  Joshua M. Lundorff ,  Sara C. McFarlan ,  Erin Marasco ,  Wei Niu ,  Fernando A. Sanchez-Riera ,  Christopher Solheid ,  David P. Weiner ,  Peter Luginbuhl ,  Analia Bueno ,  Joslin Cuenca

IPC分类号： C12P17/10 ,  C12P13/22 ,  C12P13/04 ,  C12N9/10 ,  C12P21/06 ,  C12P19/34 ,  C12N1/20 ,  C07H21/04 ,  C07K1/00

CPC分类号： C12P13/227 ,  C12N9/0004 ,  C12N9/1096 ,  C12P17/10

摘要： The invention provides for aminotransferase and oxidoreductase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such aminotransferase and oxidoreductase nucleic acids and polypeptides.

摘要翻译： 本发明提供氨基转移酶和氧化还原酶多肽以及编码这种多肽的核酸。 还提供了使用这种氨基转移酶和氧化还原酶核酸和多肽的方法。

公开(公告)号：US07582455B2

公开(公告)日：2009-09-01

申请号：US11411229

申请日：2006-04-26

申请人： Brian J. Brazeau ,  Ellen Burke ,  Mervyn DeSouza ,  Steven J. Gort ,  Paula M. Hicks ,  Sherry R. Kollmann ,  Peter Luginbuhl ,  Sara C. McFarlan ,  Toby Richardson ,  Fernando A. Sanchez-Riera ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

发明人： Brian J. Brazeau ,  Ellen Burke ,  Mervyn DeSouza ,  Steven J. Gort ,  Paula M. Hicks ,  Sherry R. Kollmann ,  Peter Luginbuhl ,  Sara C. McFarlan ,  Toby Richardson ,  Fernando A. Sanchez-Riera ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

IPC分类号： C12P17/10

CPC分类号： C12P17/10 ,  C12N9/1096 ,  C12N9/90

摘要： Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

摘要翻译： 使用多肽和生物合成途径产生莫纳甜和莫纳甜的某些立体异构体，例如R，R莫纳甜和S，R莫纳甜及其盐。 这些多肽和生物合成途径也可用于生产在特定莫纳甜合成途径中形成的包括一些生物合成途径的中间体R-2-羟基-2-（吲哚-3-基甲基）-4-酮戊二酸。