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    Aldolases, nucleic acids encoding them and methods for making and using them
    7.
    发明授权
    Aldolases, nucleic acids encoding them and methods for making and using them 有权
    醛缩酶，编码它们的核酸和制备和使用它们的方法

    公开(公告)号：US09005938B2

    公开(公告)日：2015-04-14

    申请号：US13235107

    申请日：2011-09-16

    申请人： Ellen Burke ,  Steven John Gort ,  Paula M. Hicks ,  Peter Luginbuhl ,  Sara C. McFarlan ,  Toby Richardson ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

    发明人： Ellen Burke ,  Steven John Gort ,  Paula M. Hicks ,  Peter Luginbuhl ,  Sara C. McFarlan ,  Toby Richardson ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

    IPC分类号： C12P17/10 ,  C12P13/22 ,  C12N9/10 ,  C12P21/06 ,  C12P19/34 ,  C07H21/04 ,  C12N9/88 ,  C12P13/00

    CPC分类号： C12P17/10 ,  C12N9/88 ,  C12P13/001 ,  Y02P20/52

    摘要： This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.

    摘要翻译： 本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一些实施方案中，本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和 多肽。 根据本发明的多肽可以用于各种药物，农业和工业环境。 在一些实施方案中，本发明提供了可用于制备R-2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸（R-MP）和莫纳甜的某些立体异构体的多肽和生物合成途径，例如R ，R和S，R莫纳甜及其盐，以及莫纳甜衍生物的某些立体异构体，例如R，R和S，R构型及其盐。

    ALDOLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM
    8.
    发明申请
    ALDOLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 有权
    阿尔法，核酸编码方法及其使用方法

    公开(公告)号：US20120009634A1

    公开(公告)日：2012-01-12

    申请号：US13235107

    申请日：2011-09-16

    申请人： Ellen BURKE ,  Steven John Gort ,  Paula M. Hicks ,  Peter Luginbuhl ,  Sara C. McFarlan ,  Toby Richardson ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

    发明人： Ellen BURKE ,  Steven John Gort ,  Paula M. Hicks ,  Peter Luginbuhl ,  Sara C. McFarlan ,  Toby Richardson ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

    IPC分类号： C12P17/10

    CPC分类号： C12P17/10 ,  C12N9/88 ,  C12P13/001 ,  Y02P20/52

    摘要： This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S, R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.

    摘要翻译： 本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一些实施方案中，本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和 多肽。 根据本发明的多肽可以用于各种药物，农业和工业环境。 在一些实施方案中，本发明提供了可用于制备R-2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸（R-MP）和莫纳甜的某些立体异构体的多肽和生物合成途径，例如R ，R和S，R莫纳甜及其盐，以及莫纳甜衍生物的某些立体异构体，例如R，R和S，R构型及其盐。

    Aldolases, nucleic acids encoding them and methods for making and using them
    9.
    发明授权
    Aldolases, nucleic acids encoding them and methods for making and using them 有权
    醛缩酶，编码它们的核酸和制备和使用它们的方法

    公开(公告)号：US08043837B2

    公开(公告)日：2011-10-25

    申请号：US11714909

    申请日：2007-03-07

    申请人： Ellen Burke ,  Steven John Gort ,  Paula M. Hicks ,  Peter Luginbuhl ,  Sara C. McFarlan ,  Toby Richardson ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

    发明人： Ellen Burke ,  Steven John Gort ,  Paula M. Hicks ,  Peter Luginbuhl ,  Sara C. McFarlan ,  Toby Richardson ,  Christopher Solheid ,  David Weiner ,  Lishan Zhao

    IPC分类号： C12P17/10 ,  C12P13/22 ,  C12P21/06 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12P17/10 ,  C12N9/88 ,  C12P13/001 ,  Y02P20/52

    摘要： This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.

    摘要翻译： 本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一些实施方案中，本发明涉及具有醛缩酶活性的多肽，包括丙酮酸活性，例如但不限于HMG和/或KHG醛缩酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和 多肽。 根据本发明的多肽可以用于各种药物，农业和工业环境。 在一些实施方案中，本发明提供了可用于制备R-2-羟基2-（吲哚-3-基甲基）-4-酮戊二酸（R-MP）和莫纳甜的某些立体异构体的多肽和生物合成途径，例如R ，R和S，R莫纳甜及其盐，以及莫纳甜衍生物的某些立体异构体，例如R，R和S，R构型及其盐。

    Methods and systems for increasing production of equilibrium reactions
    10.
    发明授权
    Methods and systems for increasing production of equilibrium reactions 失效
    增加平衡反应产生的方法和系统

    公开(公告)号：US07888081B2

    公开(公告)日：2011-02-15

    申请号：US11752492

    申请日：2007-05-23

    申请人： Anil Bhagwan Khare ,  Brent H. Hilbert ,  Christopher Solheid ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  Kent Floy ,  Paula M. Hicks

    发明人： Anil Bhagwan Khare ,  Brent H. Hilbert ,  Christopher Solheid ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  Kent Floy ,  Paula M. Hicks

    IPC分类号： C12P13/22 ,  C12N1/21

    CPC分类号： C07D209/20 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  C12P17/10

    摘要： Methods and systems for increasing the production of equilibrium reactions are described. In some embodiments, a method comprises removing enzymes from a reaction mixture once equilibrium is achieved, selectively re-adding enzymes and reactants for driving the product-forming reaction to product, and optionally recycling stabilized intermediates, by-products and/or co-products back into the reaction mixture. In some embodiments, a method comprises purifying the desired product from a reaction mixture and selectively recycling one or more components of the reaction mixture, such as original reactants, intermediates, co-products or by-products back into the reaction mixture as desired to improve titer. Systems for implementing the methods are also provided. In some embodiments the methods and systems are implemented for increasing the production of monatin and monatin derivatives, produced in multi-step equilibrium pathways.

    摘要翻译： 描述了用于增加平衡反应生成的方法和系统。 在一些实施方案中，一种方法包括一旦达到平衡即从反应混合物中除去酶，选择性地重新加入用于将产物形成反应驱动到产物中的酶和反应物，以及任选地再循环稳定的中间体，副产物和/或副产物 回到反应混合物中。 在一些实施方案中，方法包括从反应混合物中纯化所需产物，并根据需要选择性地将反应混合物中的一种或多种组分（例如原始反应物，中间体，副产物或副产物）循环回到反应混合物中，以改善 滴度。 还提供了实现方法的系统。 在一些实施方案中，实施方法和系统用于增加在多步平衡途径中产生的莫纳甜和莫纳甜衍生物的产生。