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    Microorganisms and methods for carbon-efficient biosynthesis of MEK and 2-butanol
    6.
    发明授权
    Microorganisms and methods for carbon-efficient biosynthesis of MEK and 2-butanol 有权
    微生物和MEK和2-丁醇的碳有效生物合成方法

    公开(公告)号：US08420375B2

    公开(公告)日：2013-04-16

    申请号：US12813469

    申请日：2010-06-10

    申请人： Robin E. Osterhout ,  Wei Niu ,  Anthony P. Burgard

    发明人： Robin E. Osterhout ,  Wei Niu ,  Anthony P. Burgard

    IPC分类号： C12N1/20 ,  C12N1/00 ,  C12N9/00 ,  C12P7/26 ,  C12P7/16 ,  C12P21/06 ,  C07H21/04

    CPC分类号： C12P7/26 ,  C12N15/52 ,  C12P7/16 ,  Y02E50/10

    摘要： A non-naturally occurring microbial organism has at least one exogenous nucleic acid encoding a MEK pathway enzyme expressed in a sufficient amount to produce MEK. The MEK pathway includes an enzyme selected from an acetoacetyl-CoA dehydrogenase (bifunctional), an acetoacetyl-CoA aldehyde dehydrogenase, a 3-oxobutyraldehyde reductase, a 3-oxobutanol dehydratase, an MEK oxidoreductase, a 3-oxobutyraldehyde aminotransferase, a 4-aminobutan-2-one deaminase, a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP aminotransferase, a 2,4-dioxopentanoate decarboxylase, an AKP deaminase, an acetylacrylate decarboxylase, an AKP decarboxylase, a glutamate dehydrogenase, a 3-oxobutyraldehyde oxidoreductase (aminating) and an AKP oxidoreductase (aminating). A 2-butanol pathway further includes an MEK reductase. A method for producing MEK or 2-butanol includes culturing these organisms under conditions and for a sufficient period of time to produce MEK or 2-butanol.

    摘要翻译： 非天然存在的微生物有至少一种编码MEK途径酶的外源核酸，其表达量足以产生MEK。 MEK途径包括选自乙酰乙酰辅酶A脱氢酶（双功能），乙酰乙酰辅酶A醛脱氢酶，3-氧代丁醛还原酶，3-氧代丁醇脱水酶，MEK氧化还原酶，3-氧代丁醛氨基转移酶，4-氨基丁酸 -2-酮脱氨酶，2-氨基-4-酮戊酸酯（AKP）硫解酶，AKP氨基转移酶，2,4-二氧代戊酸脱羧酶，AKP脱氨酶，乙酰丙酸酯脱羧酶，AKP脱羧酶，谷氨酸脱氢酶， 氧化丁醛氧化还原酶（胺化）和AKP氧化还原酶（胺化）。 2-丁醇途径还包括MEK还原酶。 包括生产MEK或2-丁醇的方法包括在条件和足够的时间内培养这些生物体以产生MEK或2-丁醇。

    COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS
    9.
    发明申请
    COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS 有权

    公开(公告)号：US20090075351A1

    公开(公告)日：2009-03-19

    申请号：US12049256

    申请日：2008-03-14

    申请人： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

    发明人： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

    IPC分类号： C12N1/21 ,  C12P7/52 ,  C12P7/18

    CPC分类号： C12N15/52 ,  B01D3/002 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12N9/93 ,  C12N15/70 ,  C12N15/81 ,  C12P7/18 ,  C12P7/42 ,  C12P7/52 ,  C12P17/04 ,  C12Y101/01061 ,  C12Y102/01076 ,  C12Y401/01071 ,  C12Y602/01004 ,  Y02P20/52

    摘要： The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or α-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric 4-hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium.