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    ROOM TEMPERATURE ELUTION OF NUCLEIC ACIDS
    1.
    发明申请
    ROOM TEMPERATURE ELUTION OF NUCLEIC ACIDS 审中-公开
    核酸的室温降解

    公开(公告)号：US20100173392A1

    公开(公告)日：2010-07-08

    申请号：US12698079

    申请日：2010-02-01

    申请人： James C. Davis ,  Michael Hogan

    发明人： James C. Davis ,  Michael Hogan

    IPC分类号： C12P19/28

    CPC分类号： C12N15/1006

    摘要： The present invention provides compositions and methods for efficient recovery of nucleic acids, in particular DNA, from storage media. The present invention provides a method for using an alkaline elution buffer and mechanical agitation to elute nucleic acids from dried samples on storage media at room temperature. In particular, the present invention provides a method for room temperature recovery of DNA, including double stranded DNA (dsDNA) and single stranded (ssDNA), from storage media. The eluted DNA is suitable for numerous downstream applications

    摘要翻译： 本发明提供了用于从存储介质有效地回收核酸，特别是DNA的组合物和方法。 本发明提供了一种使用碱性洗脱缓冲液和机械搅拌以在室温下从存储介质上从干燥样品中洗脱核酸的方法。 特别地，本发明提供了从存储介质中室温回收DNA的方法，包括双链DNA（dsDNA）和单链（ssDNA）。 洗脱的DNA适用于许多下游应用

    Multi-functional bartender's tool and related methods
    4.
    发明授权
    Multi-functional bartender's tool and related methods 有权
    多功能酒保工具及相关方法

    公开(公告)号：US08631722B1

    公开(公告)日：2014-01-21

    申请号：US12372718

    申请日：2009-02-17

    申请人： James C. Davis

    发明人： James C. Davis

    IPC分类号： B67B7/44 ,  B25F1/00

    CPC分类号： B67B7/44 ,  B25F1/04 ,  B67B7/0423 ,  B67B7/16 ,  B67B2007/0458

    摘要： Apparatus and methods are disclosed for a combination tool useful for bartending or the like. The tools combined for efficiency, convenience, and other purposes include one or more zesters, bottle openers, corkscrews, juicers, jiggers or measuring cups, knives, pouring spout, and/or others. In one embodiment, the combined tools are in a shape that is safe to the user, a size that is relatively portable and lightweight, and from materials that are non-contaminating and readily cleaned. Methods include using the tool for bartending or similar activities.

    摘要翻译： 公开了用于调酒等的组合工具的装置和方法。 结合效率，便利性和其他目的的工具包括一个或多个测试仪，开瓶器，开瓶器，榨汁机，跳汰机或量杯，刀，倾倒嘴和/或其他。 在一个实施例中，组合的工具具有对用户安全的形状，相对便携和重量轻的尺寸以及来自不污染且容易清洁的材料。 方法包括使用该工具进行调酒或类似活动。

    Sealed sample storage element system and method
    6.
    发明授权
    Sealed sample storage element system and method 有权
    密封样品储存元件系统和方法

    公开(公告)号：US07718442B2

    公开(公告)日：2010-05-18

    申请号：US10302647

    申请日：2002-11-22

    申请人： James C. Davis ,  Mitchell D. Eggers ,  John W. Sadler

    发明人： James C. Davis ,  Mitchell D. Eggers ,  John W. Sadler

    IPC分类号： G01N1/00

    CPC分类号： B01L3/50853 ,  A01N1/02 ,  A01N1/0263 ,  B01L3/545 ,  B01L2300/021 ,  B01L2300/022 ,  B01L2300/044 ,  B01L2300/0829 ,  B01L2300/0851 ,  G01N35/028 ,  G01N2001/288 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2525 ,  Y10T436/255

    摘要： A system and method operative in accordance with the present disclosure facilitate storage and retrieval of individual or discrete samples of biological, non-biological, and chemical material stored on dry media. Sample material may be disposed upon or within a porous or solid (i.e., non-porous) sample storage medium and subsequently archived in, and retrieved from, storage elements such as multi-well plates, for example, using robotic devices or other automated apparatus. The disclosed system and method enable ejection of sample material from a sealed storage element into a specific well of a multi-well daughter plate, or into a specific cuvette, test tube, or similar container. In some embodiments, a sample carrier comprising a storage medium may be punched or ejected through a first seal of the storage element with an apparatus or implement such as a disposable piercing tip, for instance, inserted through a second seal of the storage element.

    摘要翻译： 根据本公开操作的系统和方法便于存储和回收存储在干燥介质上的生物，非生物和化学材料的个体或离散样品。 样品材料可以设置在多孔或固体（即，非多孔）样品存储介质上或内部，并且随后存储在存储元件例如多孔板中并从其中取出，例如使用机器人装置或其它自动化装置 。 所公开的系统和方法使得样品材料能够从密封的存储元件排出到多孔子板的特定孔中，或者进入特定的比色皿，试管或类似的容器中。 在一些实施例中，包括存储介质的样品载体可以通过存储元件的第一密封件用诸如一次性穿刺尖端的装置或工具冲压或喷射，例如通过存储元件的第二密封件插入。

    Gas dehydration membrane with low oxygen and nitrogen permeability
    7.
    发明授权
    Gas dehydration membrane with low oxygen and nitrogen permeability 失效
    具有低氧和氮渗透性的气体脱水膜

    公开(公告)号：US6083297A

    公开(公告)日：2000-07-04

    申请号：US936157

    申请日：1997-09-24

    申请人： Ronald J. Valus ,  Randall W. Nichols ,  James C. Davis

    发明人： Ronald J. Valus ,  Randall W. Nichols ,  James C. Davis

    IPC分类号： B01D53/22 ,  B01D53/26

    CPC分类号： B01D53/228 ,  B01D53/268

    摘要： The invention relates to a gas dehydration membrane comprising a membrane having pores communicating between a first and an opposing second surface of the membrane and further having an average pore diameter on the first surface of the membrane which is about 10 to 1000 times smaller than that on the opposing second surface of the membrane. The invention further relates to a process for dehydrating a gaseous feedstock comprising passing the gaseous feedstock over a membrane characterized by high surface porosity wherein pore openings on a first surface thereof are at least 10 to 1000 times smaller than pore openings on an opposing surface thereof and wherein from about 2% to about 30% of the pore volume is occupied by a humectant.

    摘要翻译： 本发明涉及一种气体脱水膜，其包括具有在膜的第一和相对的第二表面之间连通的孔，并且膜的第一表面上的平均孔径比在膜的第一表面上的平均孔径小约10至1000倍 膜的相对的第二表面。 本发明还涉及一种用于使气态原料脱水的方法，包括使气态原料通过特征在于高表面孔隙率的膜，其中其第一表面上的孔隙比其相对表面上的孔开口小至少10至1000倍， 其中孔体积的约2％至约30％被保湿剂占据。

    High pressure facilitated membranes for selective separation and process for the use thereof
    8.
    发明授权
    High pressure facilitated membranes for selective separation and process for the use thereof 失效
    用于选择性分离的高压薄膜及其使用方法

    公开(公告)号：US5057641A

    公开(公告)日：1991-10-15

    申请号：US506721

    申请日：1990-04-09

    申请人： Ronald J. Valus ,  Reza Eshraghi ,  Alexander E. Velikoff ,  James C. Davis

    发明人： Ronald J. Valus ,  Reza Eshraghi ,  Alexander E. Velikoff ,  James C. Davis

    IPC分类号： B01D53/22

    CPC分类号： B01D53/22 ,  B01D63/02 ,  B01D2313/22 ,  B01D2313/38

    摘要： The subject invention relates to a process for the selective separation of at least one component of a gaseous feedstream comprising passing the feedstream containing the component through a separation unit, the separation unit containing a porous membrane having a feed side and a permeate side, and a pore size of from about 10 Angstroms to about 200 Angstroms, and having disposed in the pores of the porous membrane a facilitator liquid comprising a carrier dissolved in a suitable solvent, including:a) dissolving the component in the facilitator liquid on the feed side of the porous membrane at the feed gas/membrane interface;b) forming a component-carrier complex;c) diffusing the complex to the permeate side of the porous membrane; andd) releasing the component from the carrier.The invention further relates to a membrane for the selective separation of at least one component of a gaseous feedstream comprising a porous membrane, having pores of from about 10 Angstroms to about 200 Angstroms, the pores containing a facilitator liquid having an affinity for the component to be selectively separated, the membrane being capable of operating at a transmembrane pressure of about 100 psig to about 300 psig.The subject invention also relates to use of the above-identified process and membrane for the separation of vapor phase olefins from olefin-containing feed streams.

    High pressure facilitated membranes for selective separation and process for the use thereof
    10.
    发明授权
    High pressure facilitated membranes for selective separation and process for the use thereof 失效
    高压促进膜用于选择性分离和其使用方法

    公开(公告)号：US5131928A

    公开(公告)日：1992-07-21

    申请号：US667947

    申请日：1991-03-11

    申请人： Marc W. Blackman ,  Alex E. Velikoff ,  James C. Davis ,  Ronald J. Valus

    发明人： Marc W. Blackman ,  Alex E. Velikoff ,  James C. Davis ,  Ronald J. Valus

    IPC分类号： B01D53/22

    CPC分类号： B01D53/22 ,  B01D63/02 ,  B01D63/021 ,  B01D2313/22

    摘要： The subject invention relates to a membrane for the selective separation of at least one component of a gaseous feed stream comprising a porous membrane, having pores of from about 10 Angstroms to about 200 Angstroms, the pores containing a facilitator liquid having an affinity for the at least one component to be selectively separated, the membrane being capable of operating at a transmembrane pressure of about 100 psig to about 1000 psig, and the membrane comprising a polymer, a solvent, an alcohol non-solvent, and a viscosity enhancer and pore former.

    摘要翻译： 本发明涉及用于选择性分离包含多孔膜的气态进料流的至少一种成分的膜，该多孔膜具有约10埃至约200埃的孔隙，该孔隙含有对于 至少一个要选择性分离的组分，所述膜能够以约100psig至约1000psig的跨膜压力操作，并且所述膜包含聚合物，溶剂，醇非溶剂和粘度增强剂和成孔剂 。