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公开(公告)号：US20060266867A1
公开(公告)日：2006-11-30
申请号：US11140815
申请日：2005-05-31
Applicant: James Critelli , Jonathan Beckwith
Inventor： James Critelli , Jonathan Beckwith
CPC classification number: G01B3/1005 , G01B2003/103 , G01B2003/1046 , G01B2003/1048
Abstract: A tape measure includes a post with at least first and second arms spaced from one another to form a longitudinal slot having an end aperture for coupling with a tape blade retraction spring. The post distal end anchors to a housing sidewall via a fastener, and a fastener engagement region of the post extends from the aperture to the fastener's terminus. A distinct reinforcing collar is disposed about the post's distal portion and acts to limit movement of the arms away from one another. The collar may overly the post for at least half of the fastener engagement region and/or may abut a shoulder on the post. The fastener may have a first pullout resistance level without the collar present and a second higher pullout resistance level, advantageously double or more, with the collar present. Also, methods of assembly are described.
Abstract translation: 卷尺包括具有彼此间隔开的至少第一和第二臂的柱以形成具有用于与带片缩回弹簧联接的端部孔的纵向狭槽。 后远端通过紧固件锚固到壳体侧壁，并且柱的紧固件接合区域从孔延伸到紧固件的终端。 不同的加强套环设置在柱的远侧部分周围，并且用于限制臂彼此远离移动。 套环可以在紧固件接合区域的至少一半上过柱，和/或可以邻接柱上的肩部。 紧固件可以具有第一拔出阻力水平，而不存在套环，并且具有第二更高的拔出阻力水平，有利地是两倍以上。 此外，描述了组装方法。
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公开(公告)号：US20070147963A1
公开(公告)日：2007-06-28
申请号：US11614295
申请日：2006-12-21
Applicant: James Critelli , Jonathan Beckwith
Inventor： James Critelli , Jonathan Beckwith
IPC: B23D71/00
CPC classification number: B23D71/04 , B23D73/02 , B23D73/12 , C23C30/00 , Y10T407/18
Abstract: A hand operated file is provided with a thin ceramic coating of a transition metal nitride, such as vanadium nitride and/or zirconium nitride, on its teeth. The coating may be 20 um or less, thereby not altering the macro dimensions of the file. The coating may be applied using a vapor deposition process such as physical vapor deposition, and advantageously via a physical vapor deposition process where the file body does not exceed a temperature of about 300° F. The coating helps prevent galling, thereby increasing the useful life of the file.
Abstract translation: 手动文件在其牙齿上设置有诸如氮化钒和/或氮化锆的过渡金属氮化物的薄陶瓷涂层。 涂层可以是20μm或更小，因此不会改变文件的宏尺寸。 可以使用气相沉积工艺（例如物理气相沉积），并且有利地通过物理气相沉积工艺（其中文件体不超过约300°F的温度）涂覆涂层。涂层有助于防止磨损，从而增加使用寿命 的文件。
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3.发明授权公开(公告)号：US07816117B2
公开(公告)日：2010-10-19
申请号：US11411988
申请日：2006-04-26
Applicant: Jonathan Beckwith , Daniel Ritz , Melinda Faulkner , Stephanie Gon , George Georgiou
Inventor： Jonathan Beckwith , Daniel Ritz , Melinda Faulkner , Stephanie Gon , George Georgiou
CPC classification number: C12N9/0065 , C12N2500/44 , C12N2501/70 , C12N2510/02
Abstract: The invention provides composition and methods for producing proteins of interest which comprise at least one disulfide bond, include proteins which in their mature form do not contain disulfide bonds, but whose precursor molecule contained at least one disulfide bond. The methods employ a host cell modified to more efficiently produce properly folded disulfide bond containing proteins. The host cells generally contain a mutation in one or more reductase genes, and can be further genetically modified to increase their growth rate, and are further optionally modified to increase the expression of a catalyst of disulfide bond formation. Host cells, methods for u sing such to produce proteins of interest, proteins of interest produced by these methods are within the scope of the invention.
Abstract translation: 本发明提供用于产生包含至少一个二硫键的感兴趣蛋白质的组合物和方法，包括其成熟形式不含二硫键但其前体分子含有至少一个二硫键的蛋白质。 该方法使用修饰的宿主细胞以更有效地产生适当折叠的含二硫键的蛋白质。 宿主细胞通常在一个或多个还原酶基因中含有突变，并且可进行进一步遗传修饰以增加其生长速率，并进一步任选地修饰以增加二硫键形成催化剂的表达。 宿主细胞，用于产生感兴趣的蛋白质的方法，通过这些方法生产的感兴趣的蛋白质在本发明的范围内。
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4.发明授权Compositions and methods for production of disulfide bond containing proteins in host cells 有权用于在宿主细胞中生产含二硫键的蛋白质的组合物和方法公开(公告)号：US07410788B2
公开(公告)日：2008-08-12
申请号：US11058926
申请日：2005-02-16
Applicant: Jonathan Beckwith , Fredrik Aslund , Paul H. Bessette , George Georgiou , Daniel Ritz , Jackie Eun-ah Lim
Inventor： Jonathan Beckwith , Fredrik Aslund , Paul H. Bessette , George Georgiou , Daniel Ritz , Jackie Eun-ah Lim
CPC classification number: C12N9/0004 , C12P21/02
Abstract: The invention provides composition and methods for producing proteins of interest which comprise at least one disulfide bond, include proteins which in their mature form do not contain disulfide bonds, but whose precursor molecule contained at least one disulfide bond. The methods employ a host cell modified to more efficiently produce properly folded disulfide bond containing proteins. The host cells generally contain a mutation in one or more reductase genes, and can be further genetically modified to increase their growth rate, and are further optionally modified to increase the expression of a catalyst of disulfide bond formation. Host cells, methods for using such to produce proteins of interest, proteins of interest produced by these methods are within the scope of the invention.
Abstract translation: 本发明提供用于产生包含至少一个二硫键的感兴趣蛋白质的组合物和方法，包括其成熟形式不含二硫键但其前体分子含有至少一个二硫键的蛋白质。 该方法使用修饰的宿主细胞以更有效地产生适当折叠的含二硫键的蛋白质。 宿主细胞通常在一个或多个还原酶基因中含有突变，并且可进行进一步遗传修饰以增加其生长速率，并进一步任选地修饰以增加二硫键形成催化剂的表达。 宿主细胞，使用其产生感兴趣的蛋白质的方法，通过这些方法生产的感兴趣的蛋白质在本发明的范围内。
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公开(公告)号：US20110243958A1
公开(公告)日：2011-10-06
申请号：US13123470
申请日：2009-10-14
Applicant: Jonathan Beckwith , Rachel Dutton , Dana Boyd , Mehmet Berkmen
Inventor： Jonathan Beckwith , Rachel Dutton , Dana Boyd , Mehmet Berkmen
CPC classification number: A61K31/192 , A61K31/37 , C12Q1/18 , G01N2333/35 , G01N2333/904 , Y02A50/473
Abstract: Aspects of the invention relate to a method for inhibiting the growth of a microbe that expresses bacterial vitamin K epoxide reductase (bVKOR). The method involves contacting the bacterial cell with an effective amount of an agent that inhibits bVKOR. Agents include a drug, ligand or portion thereof, protein, polypeptide, small organic molecule, antisense nucleic acid, RNAi, or antibody. Examples of useful agents are a phenylpropanoid, a modified phenylpropanoid, a coumarin or modified coumarin. A particularly useful agents is warfarin or a variant thereof or ferulenol or a variant thereof. The microbe is any microbe carrying a bVKOR gene, such as Mycobacterium tuberculosis.
Abstract translation: 本发明的方面涉及抑制表达细菌维生素K环氧化物还原酶（bVKOR）的微生物生长的方法。 该方法包括使细菌细胞与有效量的抑制bVKOR的药剂接触。 药剂包括药物，配体或其部分，蛋白质，多肽，小有机分子，反义核酸，RNAi或抗体。 有用的试剂的实例是苯丙素，改性苯丙素，香豆素或改性香豆素。 特别有用的药剂是华法林或其变体或阿魏酸或其变体。 微生物是携带bVKOR基因的任何微生物，如结核分枝杆菌（Mycobacterium tuberculosis）。
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Patent Agency Ranking
公开(公告)号：US4914025A
公开(公告)日：1990-04-03
申请号：US805486
申请日：1985-12-05
Applicant: Colin Manoil , Jonathan Beckwith , Michael Syvanen , Ralph R. Isberg , Charles S. Hoffman , Andrew Wright
Inventor： Colin Manoil , Jonathan Beckwith , Michael Syvanen , Ralph R. Isberg , Charles S. Hoffman , Andrew Wright
CPC classification number: C12N15/10 , C12N15/625 , C07K2319/02 , C07K2319/61 , Y10S435/848 , Y10S435/849
Abstract: An export sequence of export DNA can be identified by transforming a population of cells with a vector having a transposon that includes a structural gene encoding a detectable compound, positioned between insertion sequences. The structural gene encodes a detectable compound that, in wild-type organisms, is translated with an export peptide effective to export the compound. Since the transposon lacks DNA coding for an export sequence capable of exporting the detectable gene product, transformants that export the gene product include a DNA fusion of the transposon to export DNA from the parent cell, in a position to allow expression of the fused DNA. The transformants are analyzed to locate the export DNA or a gene comprising it, and the position of the export DNA in the cell's genome is determined; the orientation of the insertion also is determined. Having identified cell genes that naturally contain export DNA, the export DNA is cloned and fused to a gene encoding a product whose production and export are desired. For example, a cell is engineered to produce and export a desired product, to enable easier substance recovery and to improve cell tolerance for high levels of the desired product.
Abstract translation: 可以通过用含有位于插入序列之间的包含编码可检测化合物的结构基因的转座子转化细胞群来鉴定出口DNA的出口序列。 结构基因编码可检测的化合物，其在野生型生物体中用有效导出化合物的出口肽翻译。 由于转座子缺乏编码能够输出可检测基因产物的出口序列的DNA，所以导出基因产物的转化子包括转座子的DNA融合，以从母细胞输出DNA，以允许表达融合的DNA。 分析转化体以定位出口DNA或包含其的基因，确定出口DNA在细胞基因组中的位置; 插入的方向也被确定。 鉴定出天然含有出口DNA的细胞基因后，将出口DNA克隆并融合到编码其生产和出口的产品的基因上。 例如，将细胞工程化以产生和输出所需产物，以使更容易的物质回收和改善对高水平所需产物的细胞耐受性。
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7.发明申请公开(公告)号：US20070254334A1
公开(公告)日：2007-11-01
申请号：US11411988
申请日：2006-04-26
Applicant: Jonathan Beckwith , Daniel Ritz , Melinda Faulkner , Stephanie Gon , George Georgiou
Inventor： Jonathan Beckwith , Daniel Ritz , Melinda Faulkner , Stephanie Gon , George Georgiou
CPC classification number: C12N9/0065 , C12N2500/44 , C12N2501/70 , C12N2510/02
Abstract: The invention provides composition and methods for producing proteins of interest which comprise at least one disulfide bond, include proteins which in their mature form do not contain disulfide bonds, but whose precursor molecule contained at least one disulfide bond. The methods employ a host cell modified to more efficiently produce properly folded disulfide bond containing proteins. The host cells generally contain a mutation in one or more reductase genes, and can be further genetically modified to increase their growth rate, and are further optionally modified to increase the expression of a catalyst of disulfide bond formation. Host cells, methods for u sing such to produce proteins of interest, proteins of interest produced by these methods are within the scope of the invention.
Abstract translation: 本发明提供用于产生包含至少一个二硫键的感兴趣蛋白质的组合物和方法，包括其成熟形式不含二硫键但其前体分子含有至少一个二硫键的蛋白质。 该方法使用修饰的宿主细胞以更有效地产生适当折叠的含二硫键的蛋白质。 宿主细胞通常在一个或多个还原酶基因中含有突变，并且可进行进一步遗传修饰以增加其生长速率，并进一步任选地修饰以增加二硫键形成催化剂的表达。 宿主细胞，用于产生感兴趣的蛋白质的方法，通过这些方法生产的感兴趣的蛋白质在本发明的范围内。
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公开(公告)号：US20070251019A1
公开(公告)日：2007-11-01
申请号：US11770304
申请日：2007-06-28
Applicant: Jonathan Beckwith , Alan Anderson
Inventor： Jonathan Beckwith , Alan Anderson
CPC classification number: B25B13/14
Abstract: An adjustable wrench includes: a head having a first fixed jaw; a handle extending away from the head; a selectively positionable second jaw; an adjustment mechanism that includes a rotatable shaft, an actuator, and a gear train operatively coupling the shaft to the second jaw; the shaft having an external spiral groove and disposed at least partially in the handle; the actuator slidable relative to the handle and engaging the groove; first and second flanges spaced from one another and each circumferentially surrounding the shaft; and a retainer disposed between the flanges and engaging the shaft so as to limit movement of the shaft parallel to the handle longitudinal axis in two directions via abutment with the first and second flanges. Such a wrench may have a proof torque of at least the minimum level set forth in ASME B107.8, 2003 revision. Other wrenches are also disclosed.
Abstract translation: 可调扳手包括：具有第一固定夹爪的头部; 远离头部的手柄; 选择性定位的第二钳口; 调节机构，其包括可旋转轴，致动器和将所述轴可操作地联接到所述第二钳口的齿轮系; 所述轴具有外部螺旋槽并且至少部分地设置在所述手柄中; 所述致动器相对于所述手柄滑动并与所述凹槽接合; 第一和第二凸缘彼此分开并且每个周向地围绕轴; 以及保持器，其设置在所述凸缘之间并且接合所述轴，以便通过与所述第一和第二凸缘邻接来限制所述轴在两个方向上平行于所述手柄纵向轴线的移动。 这样的扳手可以具有至少在ASME B107.8,2003修订版中规定的最小等级的证明扭矩。 其他扳手也被披露。
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9.发明授权Compositions and methods for production of disulfide bond containing proteins in host cells 有权用于在宿主细胞中生产含二硫键的蛋白质的组合物和方法公开(公告)号：US06872563B1
公开(公告)日：2005-03-29
申请号：US09679705
申请日：2000-10-05
Applicant: Jonathan Beckwith , Fredrik Aslund , Paul H. Bessette , George Georgiou , Daniel Ritz , Jackie Eun-ah Lim
Inventor： Jonathan Beckwith , Fredrik Aslund , Paul H. Bessette , George Georgiou , Daniel Ritz , Jackie Eun-ah Lim
CPC classification number: C12N9/0004 , C12P21/02
Abstract: The invention provides composition and methods for producing proteins of interest which comprise at least one disulfide bond, include proteins which in their mature form do not contain disulfide bonds, but whose precursor molecule contained at least one disulfide bond. The methods employ a host cell modified to more efficiently produce properly folded disulfide bond containing proteins. The host cells generally contain a mutation in one or more reductase genes, and can be further genetically modified to increase their growth rate, and are further optionally modified to increase the expression of a catalyst of disulfide bond formation. Host cells, methods for using such to produce proteins of interest, proteins of interest produced by these methods are within the scope of the invention.
Abstract translation: 本发明提供用于产生包含至少一个二硫键的感兴趣蛋白质的组合物和方法，包括其成熟形式不含二硫键但其前体分子含有至少一个二硫键的蛋白质。 该方法使用修饰的宿主细胞以更有效地产生适当折叠的含二硫键的蛋白质。 宿主细胞通常在一个或多个还原酶基因中含有突变，并且可进行进一步遗传修饰以增加其生长速率，并进一步任选地修饰以增加二硫键形成催化剂的表达。 宿主细胞，使用其产生感兴趣的蛋白质的方法，通过这些方法生产的感兴趣的蛋白质在本发明的范围内。
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公开(公告)号：US20070125205A1
公开(公告)日：2007-06-07
申请号：US11294170
申请日：2005-12-05
Applicant: Jonathan Beckwith , Alan Anderson
Inventor： Jonathan Beckwith , Alan Anderson
IPC: B25B13/16
CPC classification number: B25B13/14
Abstract: An adjustable wrench includes: a head having a first fixed jaw; a handle extending away from the head and having a longitudinal axis; a second jaw selectively positionable with respect the first jaw; an adjustment mechanism comprising a rotatable shaft, an actuator, and a gear train operatively coupling the shaft to the second jaw; the shaft having an external spiral groove and disposed at least partially in the handle; the actuator slidable relative to the handle and engaging the groove; first and second flanges spaced from one another; and a retainer disposed between the flanges and engaging the shaft so as to limit movement of the shaft parallel to the handle longitudinal axis in two directions via abutment with the first and second flanges. Such a wrench may have a proof torque of at least the minimum level set forth in ASME B107.8, 2003 revision.
Abstract translation: 可调扳手包括：具有第一固定夹爪的头部; 手柄，其远离头部延伸并具有纵向轴线; 相对于所述第一钳口选择性地定位的第二钳口; 调节机构，包括可旋转轴，致动器和可操作地将轴连接到第二钳口的齿轮系; 所述轴具有外部螺旋槽并且至少部分地设置在所述手柄中; 所述致动器相对于所述手柄滑动并与所述凹槽接合; 第一和第二凸缘彼此间隔开; 以及保持器，其设置在所述凸缘之间并且接合所述轴，以便通过与所述第一和第二凸缘邻接来限制所述轴在两个方向上平行于所述手柄纵向轴线的移动。 这样的扳手可以具有至少在ASME B107.8,2003修订版中规定的最小等级的证明扭矩。
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