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公开(公告)号：US20050123991A1
公开(公告)日：2005-06-09
申请号：US11049152
申请日：2005-02-02
申请人： Jay Short , Eric Mathur , Markus Baumann , Uwe Bornscheuer
发明人： Jay Short , Eric Mathur , Markus Baumann , Uwe Bornscheuer
IPC分类号： G01N33/53 , C07K16/40 , C12N9/14 , C12N9/18 , C12N9/20 , C12N15/09 , C12P21/08 , C12P41/00 , C12Q1/34 , C12Q1/68 , G01N33/566 , C07H21/04 , C12P7/18 , C12P7/40
摘要： The invention relates to hydrolases and to polynucleotides encoding the hydrolases. In addition, the invention relates to the use of these hydrolase enzymes in kinetic resolution.
摘要翻译： 本发明涉及水解酶和编码水解酶的多核苷酸。 此外，本发明涉及这些水解酶在动力学拆分中的用途。
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2.发明申请METHODS FOR USING POLYPEPTIDES HAVING AMYLASE ACTIVITY AND NUCLEIC ACIDS ENCODING THEM 有权使用具有AMYLASE活性的多肽和编码其的核酸的方法公开(公告)号：US20070161099A1
公开(公告)日：2007-07-12
申请号：US11621543
申请日：2007-01-09
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2414 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/16 , Y02E50/17
摘要： The invention relates to uses of polypeptides having amylase activity, nucleic acids encoding them, and methods for making and using them. For example, in one aspect, the invention provides methods for making alcohol using polypeptides of the invention. In one aspect, the polypeptides having amylase activity have increased activity and stability at acidic, neutral and alkaline pH and increased temperature. In one aspect, the invention provides methods for producing an alcohol, for producing a high-maltose or a high-glucose syrup or a mixed syrup, for liquifying a starch-comprising composition, for hydrolyzing a starch, comprising use of polypeptides of the invention, or polypeptides encoded by the nucleic acids of this invention.
摘要翻译： 本发明涉及具有淀粉酶活性的多肽的用途，编码它们的核酸以及制备和使用它们的方法。 例如，一方面，本发明提供了使用本发明多肽制备醇的方法。 一方面，具有淀粉酶活性的多肽在酸性，中性和碱性pH和升高的温度下具有增加的活性和稳定性。 一方面，本发明提供用于生产高麦芽糖或高葡萄糖浆或混合糖浆的醇的方法，用于液化包含淀粉的组合物，用于水解淀粉，包括使用本发明的多肽 ，或由本发明的核酸编码的多肽。
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3.发明申请公开(公告)号：US20050186605A1
公开(公告)日：2005-08-25
申请号：US11047257
申请日：2005-01-31
申请人： Jay Short , Eric Mathur , Edd Lee , Edward Bylina
发明人： Jay Short , Eric Mathur , Edd Lee , Edward Bylina
CPC分类号： C12N9/16
摘要： The invention relates to thermostable phosphatases and to polynucleotides encoding the thermostable phosphatases. In addition methods of designing new phosphatases and methods of use thereof are also provided. The thermostable phosphatases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及热稳定性磷酸酶和编码热稳定性磷酸酶的多核苷酸。 此外，还提供了设计新的磷酸酶的方法及其使用方法。 热稳定性磷酸酶在增加的pH和温度下具有增加的活性和稳定性。
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4.发明申请NUCLEIC ACIDS ENCODING POLYPEPTIDES HAVING AMYLASE ACTIVITY AND METHODS OF MAKING AND USING THEM 有权编码具有淀粉酶活性的多肽的核酸及其制备和使用方法公开(公告)号：US20080047037A1
公开(公告)日：2008-02-21
申请号：US11621528
申请日：2007-01-09
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2414 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/16 , Y02E50/17
摘要： The invention relates to nucleic acids encoding polypeptides having amylase activity, and methods for making and using them. In one aspect, the polypeptides having amylase activity have increased activity and stability at acidic, neutral and alkaline pH and increased temperature. In one aspect, the invention provides methods for producing an alcohol, for producing a high-maltose or a high-glucose syrup or a mixed syrup, for liquifying a starch-comprising composition, comprising use of polypeptides encoded by the nucleic acids of this invention.
摘要翻译： 本发明涉及编码具有淀粉酶活性的多肽的核酸，以及制备和使用它们的方法。 一方面，具有淀粉酶活性的多肽在酸性，中性和碱性pH和升高的温度下具有增加的活性和稳定性。 一方面，本发明提供用于生产用于液化含淀粉组合物的高麦芽糖或高葡萄糖浆或混合糖浆的醇的方法，其包括使用由本发明的核酸编码的多肽 。
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5.发明申请公开(公告)号：US20100209995A1
公开(公告)日：2010-08-19
申请号：US12697907
申请日：2010-02-01
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Carl Miller , Martin Kazaoka , Eric Mathur , Jay Short
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Carl Miller , Martin Kazaoka , Eric Mathur , Jay Short
IPC分类号： C12N9/26
CPC分类号： C12N9/2411 , C12N9/2417 , C12P19/14
摘要： In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars.
摘要翻译： 一方面，本发明涉及具有淀粉酶活性的多肽，编码多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一个方面，本发明的多肽可用作淀粉酶，例如α-淀粉酶，以催化淀粉水解成糖。
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6.发明申请POLYPEPTIDES HAVING AMYLASE ACTIVITY AND METHODS OF MAKING AND USING THEM 有权具有AMYLASE活性的多肽和制备和使用它们的方法公开(公告)号：US20080032378A1
公开(公告)日：2008-02-07
申请号：US11621534
申请日：2007-01-09
申请人： Walter CALLEN , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
发明人： Walter CALLEN , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2414 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/16 , Y02E50/17
摘要： This invention relates generally to polypeptides and peptides having an amylase activity, e.g., enzymes, polynucleotides encoding the enzymes, the use of such polypeptides and peptides. In one aspect, the polypeptides having amylase activity have increased activity and stability at acidic, neutral and alkaline pH and increased temperature. In one aspect, the invention provides methods for producing an alcohol, for producing a high-maltose or a high-glucose syrup or a mixed syrup, for liquifying a starch-comprising composition, comprising use of polypeptides having amylase activity of the invention.
摘要翻译： 本发明一般涉及具有淀粉酶活性的多肽和肽，例如酶，编码酶的多核苷酸，这种多肽和肽的用途。 一方面，具有淀粉酶活性的多肽在酸性，中性和碱性pH和升高的温度下具有增加的活性和稳定性。 一方面，本发明提供了用于生产用于液化包含淀粉的组合物的高麦芽糖或高葡萄糖浆或混合糖浆的醇的方法，其包括使用具有本发明的具有淀粉酶活性的多肽。
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7.发明申请Enyzmes having alpha amylase activity and methods of making and using them 有权具有α淀粉酶活性的Enyzmes及其制备和使用方法公开(公告)号：US20060234280A1
公开(公告)日：2006-10-19
申请号：US11400030
申请日：2006-04-06
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Carl Miller , Martin Kazaoka , Jay Short , Eric Mathur
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Carl Miller , Martin Kazaoka , Jay Short , Eric Mathur
IPC分类号： C12Q1/68 , A23K1/18 , C07H21/04 , C12P21/06 , C12P19/02 , C12P7/06 , C12N9/32 , C12N1/21 , C11D3/386
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2414 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/16 , Y02E50/17
摘要： The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases, and methods of making and using them. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及α-淀粉酶和编码α-淀粉酶的多核苷酸，以及制备和使用它们的方法。 另外还提供了设计新的α-淀粉酶的方法及其使用方法。 α淀粉酶在增加的pH和温度下具有增加的活性和稳定性。
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8.发明申请Amylases, nucleic acids encoding them and methods for making and using them 有权淀粉酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US20050176000A1
公开(公告)日：2005-08-11
申请号：US10489510
申请日：2003-03-24
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Carl Miller , Martin Kazaoka , Eric Mathur , Jay Short
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Carl Miller , Martin Kazaoka , Eric Mathur , Jay Short
CPC分类号： C12N9/2411 , C12N9/2417 , C12P19/14
摘要： In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for malting and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into 10 sugars.
摘要翻译： 一方面，本发明涉及具有淀粉酶活性的多肽，编码多肽的多核苷酸，以及用于发芽和使用这些多核苷酸和多肽的方法。 一方面，本发明的多肽可用作淀粉酶，例如α-淀粉酶，以催化淀粉水解成10个糖。
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公开(公告)号：US08338131B2
公开(公告)日：2012-12-25
申请号：US12697907
申请日：2010-02-01
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Carl Miller , Martin Kazaoka , Eric Mathur , Jay Short
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Carl Miller , Martin Kazaoka , Eric Mathur , Jay Short
CPC分类号： C12N9/2411 , C12N9/2417 , C12P19/14
摘要： In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars.
摘要翻译： 一方面，本发明涉及具有淀粉酶活性的多肽，编码多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一个方面，本发明的多肽可用作淀粉酶，例如α-淀粉酶，以催化淀粉水解成糖。
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10.发明申请Phytases, nucleic acids encoding them and methods for making and using them 有权植酸酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US20050246780A1
公开(公告)日：2005-11-03
申请号：US11056354
申请日：2005-02-11
申请人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , Eric Mathur
发明人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , Eric Mathur
IPC分类号： A01H1/00 , A01K67/00 , A21D8/04 , A23J3/14 , A23K1/14 , A23K1/165 , A23L1/015 , A23L1/03 , A23L1/105 , A23L1/211 , A61K38/00 , C07H21/04 , C12N1/21 , C12N5/04 , C12N9/16 , C12N15/82
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.
摘要翻译： 本发明提供了分离和重组的植酸酶。 一方面，通过修饰大肠杆菌的野生型appA产生植酸酶。 该酶可以从重组宿主细胞产生。 本发明的植酸酶可以用于有助于在需要时消化植酸盐。 特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。 本发明的植酸酶可以是耐热和/或耐热的。 还提供了获得编码植酸酶的变体多核苷酸和在高温或低温下获得具有热稳定性或耐热性的植酸酶的方法。
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