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1.发明申请Phytases, nucleic acids encoding them and methods of making and using them 有权植酸酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US20050281792A1
公开(公告)日：2005-12-22
申请号：US10933115
申请日：2004-09-01
申请人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , William Baum , Dan Robertson , Paul Zorner
发明人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , William Baum , Dan Robertson , Paul Zorner
IPC分类号： A21D8/04 , A23J3/14 , A23K1/14 , A23K1/165 , A23L1/015 , A23L1/03 , A23L1/105 , A23L1/211 , A61K38/00 , C12N1/21 , C12N9/16 , A61K45/00 , A61K38/46
CPC分类号： C12N9/16 , A01K2217/05 , A21D8/042 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K33/00 , A61K36/00 , A61K38/00 , A61K45/06 , C12Y301/03008 , C12Y301/03026 , Y02P60/877 , A23V2300/21
摘要： In one aspect, the invention provides a purified and modified phytase enzyme from Escherichia coli K12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译： 一方面，本发明提供了来自大肠杆菌K12 appA植酸酶的纯化和修饰的植酸酶。 与野生型酶相比，该酶具有植酸酶活性和改善的耐热性。 此外，该酶在低pH下具有改善的蛋白酶稳定性。 修饰的植酸酶的糖基化提供了具有改善的热耐受性和蛋白酶稳定性的进一步改进的酶。 该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。 一方面，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。
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2.发明申请Phytases, nucleic acids encoding them and methods for making and using them 有权植酸酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US20050246780A1
公开(公告)日：2005-11-03
申请号：US11056354
申请日：2005-02-11
申请人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , Eric Mathur
发明人： Jay Short , Keith Kretz , Kevin Gray , Nelson Barton , James Garrett , Eileen O'Donoghue , Eric Mathur
IPC分类号： A01H1/00 , A01K67/00 , A21D8/04 , A23J3/14 , A23K1/14 , A23K1/165 , A23L1/015 , A23L1/03 , A23L1/105 , A23L1/211 , A61K38/00 , C07H21/04 , C12N1/21 , C12N5/04 , C12N9/16 , C12N15/82
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.
摘要翻译： 本发明提供了分离和重组的植酸酶。 一方面，通过修饰大肠杆菌的野生型appA产生植酸酶。 该酶可以从重组宿主细胞产生。 本发明的植酸酶可以用于有助于在需要时消化植酸盐。 特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。 本发明的植酸酶可以是耐热和/或耐热的。 还提供了获得编码植酸酶的变体多核苷酸和在高温或低温下获得具有热稳定性或耐热性的植酸酶的方法。
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3.发明授权Amylases, nucleic acids encoding them and methods for making and using them 有权淀粉酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US07759093B2
公开(公告)日：2010-07-20
申请号：US10547957
申请日：2004-03-08
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Kevin Gray , Janne S. Kerovuo , Malgorzata Slupska , Nelson Barton , Eileen O'Donoghue , Carl Miller
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Kevin Gray , Janne S. Kerovuo , Malgorzata Slupska , Nelson Barton , Eileen O'Donoghue , Carl Miller
CPC分类号： A21D8/042 , A61K38/00 , C12C5/004 , C12N9/2411 , C12N9/2414 , C12N9/2428 , C12P19/14 , C12P19/16 , C12P19/20 , Y02E50/17
摘要： In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating.
摘要翻译： 一方面，本发明涉及具有淀粉酶活性的多肽，编码多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一个方面，本发明的多肽可用作淀粉酶，例如α-淀粉酶，以催化淀粉水解成糖。 一方面，本发明提供包含通过胶乳聚合物涂层涂覆的所需成分的延迟释放组合物。
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4.发明申请Amylases, nucleic acids encoding them and methods for making and using them 有权淀粉酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US20070157329A1
公开(公告)日：2007-07-05
申请号：US10547957
申请日：2004-03-08
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Kevin Gray , Janne Kerovuo , Malgorzata Slupska , Nelson Barton , Eileen O'Donoghue , Carl Miller
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Kevin Gray , Janne Kerovuo , Malgorzata Slupska , Nelson Barton , Eileen O'Donoghue , Carl Miller
IPC分类号： A01K67/027 , C12Q1/68 , C07H21/04 , C12P21/06 , A61K38/47 , C12P19/02 , C12N9/32 , A21D8/02 , C12C11/00 , C12P7/06 , C12N15/82 , C12N5/06
CPC分类号： A21D8/042 , A61K38/00 , C12C5/004 , C12N9/2411 , C12N9/2414 , C12N9/2428 , C12P19/14 , C12P19/16 , C12P19/20 , Y02E50/17
摘要： In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for malting and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating.
摘要翻译： 一方面，本发明涉及具有淀粉酶活性的多肽，编码多肽的多核苷酸，以及用于发芽和使用这些多核苷酸和多肽的方法。 在一个方面，本发明的多肽可用作淀粉酶，例如α-淀粉酶，以催化淀粉水解成糖。 一方面，本发明提供包含通过胶乳聚合物涂层涂覆的所需成分的延迟释放组合物。
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5.发明申请Glucosidases, nucleic acids encoding them and methods for making and using them 审中-公开葡糖苷酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US20070056053A1
公开(公告)日：2007-03-08
申请号：US10550155
申请日：2004-03-18
申请人： Kevin Gray , James Garrett , Nahla Aboushadi , Ryan Knowles , Eileen O'Donoghue , Elizabeth Waters
发明人： Kevin Gray , James Garrett , Nahla Aboushadi , Ryan Knowles , Eileen O'Donoghue , Elizabeth Waters
CPC分类号： C12N15/8257 , A01K2217/05 , C12C1/027 , C12C5/004 , C12N9/2405 , C12N15/8245
摘要： The invention is directed to polypeptides having a glucosidase activity, including an alpha-glucosidase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention are used as alpha-glucosidases to catalyze the hydrolysis of starch into sugars, e.g., to convert liquefied starch to glucose. In one aspect, the polypeptides of the invention can catalyze the hydrolysis of both alpha-(1,4) and alpha-(1,6) glucose linkages. In one aspect, the polypeptides of the invention can catalyze the hydrolysis of both malto-oligosaccharides and liquefied starch.
摘要翻译： 本发明涉及具有葡糖苷酶活性的多肽，包括α-葡糖苷酶活性，编码多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一个方面，本发明的多肽用作α-葡糖苷酶以催化淀粉水解成糖，例如将液化的淀粉转化成葡萄糖。 在一个方面，本发明的多肽可以催化α-（1,4）和α-（1,6）葡萄糖键的水解。 一方面，本发明的多肽可以催化麦芽低聚糖和液化淀粉的水解。
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公开(公告)号：US20060275804A1
公开(公告)日：2006-12-07
申请号：US11418828
申请日：2006-05-05
申请人： Jay Short , Toby Richardson , Dan Robertson , Kevin Gray
发明人： Jay Short , Toby Richardson , Dan Robertson , Kevin Gray
IPC分类号： C12Q1/68 , C07H21/04 , C12P21/06 , C12P7/40 , C12N9/02 , C12N1/21 , C12N1/18 , C12N5/04 , C12N5/06
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature.
摘要翻译： 本发明涉及卤代烷烃脱卤素酶和编码卤代烷烃脱卤素酶的多核苷酸。 另外还提供了设计新的脱卤素酶的方法及其使用方法。 脱盐酶在增加的pH和温度下具有增加的活性和稳定性。
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公开(公告)号：US20060035216A1
公开(公告)日：2006-02-16
申请号：US10000997
申请日：2001-11-30
申请人： Jay Short , Toby Richardson , Dan Robertson , Kevin Gray
发明人： Jay Short , Toby Richardson , Dan Robertson , Kevin Gray
CPC分类号： C12N9/14 , G06F19/22 , Y10S422/90
摘要： The invention relates to haloalkane dehalogenases and to polynucleotides encoding alkane dehalogenases. In addition methods of designing new dehalogenases and of use thereof are also provided. The dehalogenases have increased activity and at increased pH and temperature.
摘要翻译： 本发明涉及卤烷烃脱卤酶和编码烷烃脱卤酶的多核苷酸。 此外，还提供了设计新的脱卤素酶及其用途的方法。 脱卤酶具有增加的活性和增加的pH和温度。
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8.发明申请POLYPEPTIDES HAVING AMYLASE ACTIVITY AND METHODS OF MAKING AND USING THEM 有权具有AMYLASE活性的多肽和制备和使用它们的方法公开(公告)号：US20080032378A1
公开(公告)日：2008-02-07
申请号：US11621534
申请日：2007-01-09
申请人： Walter CALLEN , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
发明人： Walter CALLEN , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2414 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/16 , Y02E50/17
摘要： This invention relates generally to polypeptides and peptides having an amylase activity, e.g., enzymes, polynucleotides encoding the enzymes, the use of such polypeptides and peptides. In one aspect, the polypeptides having amylase activity have increased activity and stability at acidic, neutral and alkaline pH and increased temperature. In one aspect, the invention provides methods for producing an alcohol, for producing a high-maltose or a high-glucose syrup or a mixed syrup, for liquifying a starch-comprising composition, comprising use of polypeptides having amylase activity of the invention.
摘要翻译： 本发明一般涉及具有淀粉酶活性的多肽和肽，例如酶，编码酶的多核苷酸，这种多肽和肽的用途。 一方面，具有淀粉酶活性的多肽在酸性，中性和碱性pH和升高的温度下具有增加的活性和稳定性。 一方面，本发明提供了用于生产用于液化包含淀粉的组合物的高麦芽糖或高葡萄糖浆或混合糖浆的醇的方法，其包括使用具有本发明的具有淀粉酶活性的多肽。
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9.发明申请METHODS FOR USING POLYPEPTIDES HAVING AMYLASE ACTIVITY AND NUCLEIC ACIDS ENCODING THEM 有权使用具有AMYLASE活性的多肽和编码其的核酸的方法公开(公告)号：US20070161099A1
公开(公告)日：2007-07-12
申请号：US11621543
申请日：2007-01-09
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2414 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/16 , Y02E50/17
摘要： The invention relates to uses of polypeptides having amylase activity, nucleic acids encoding them, and methods for making and using them. For example, in one aspect, the invention provides methods for making alcohol using polypeptides of the invention. In one aspect, the polypeptides having amylase activity have increased activity and stability at acidic, neutral and alkaline pH and increased temperature. In one aspect, the invention provides methods for producing an alcohol, for producing a high-maltose or a high-glucose syrup or a mixed syrup, for liquifying a starch-comprising composition, for hydrolyzing a starch, comprising use of polypeptides of the invention, or polypeptides encoded by the nucleic acids of this invention.
摘要翻译： 本发明涉及具有淀粉酶活性的多肽的用途，编码它们的核酸以及制备和使用它们的方法。 例如，一方面，本发明提供了使用本发明多肽制备醇的方法。 一方面，具有淀粉酶活性的多肽在酸性，中性和碱性pH和升高的温度下具有增加的活性和稳定性。 一方面，本发明提供用于生产高麦芽糖或高葡萄糖浆或混合糖浆的醇的方法，用于液化包含淀粉的组合物，用于水解淀粉，包括使用本发明的多肽 ，或由本发明的核酸编码的多肽。
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10.发明申请NUCLEIC ACIDS ENCODING POLYPEPTIDES HAVING AMYLASE ACTIVITY AND METHODS OF MAKING AND USING THEM 有权编码具有淀粉酶活性的多肽的核酸及其制备和使用方法公开(公告)号：US20080047037A1
公开(公告)日：2008-02-21
申请号：US11621528
申请日：2007-01-09
申请人： Walter Callen , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
发明人： Walter Callen , Toby Richardson , Gerhard Frey , Jay Short , Eric Mathur , Kevin Gray , Janne Kerovuo , Malgorzata Slupska
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2414 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/16 , Y02E50/17
摘要： The invention relates to nucleic acids encoding polypeptides having amylase activity, and methods for making and using them. In one aspect, the polypeptides having amylase activity have increased activity and stability at acidic, neutral and alkaline pH and increased temperature. In one aspect, the invention provides methods for producing an alcohol, for producing a high-maltose or a high-glucose syrup or a mixed syrup, for liquifying a starch-comprising composition, comprising use of polypeptides encoded by the nucleic acids of this invention.
摘要翻译： 本发明涉及编码具有淀粉酶活性的多肽的核酸，以及制备和使用它们的方法。 一方面，具有淀粉酶活性的多肽在酸性，中性和碱性pH和升高的温度下具有增加的活性和稳定性。 一方面，本发明提供用于生产用于液化含淀粉组合物的高麦芽糖或高葡萄糖浆或混合糖浆的醇的方法，其包括使用由本发明的核酸编码的多肽 。
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