公开(公告)号：US07855069B2

公开(公告)日：2010-12-21

申请号：US11253541

申请日：2005-10-19

申请人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

发明人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

IPC分类号： C12M1/42 ,  C12M1/33

CPC分类号： C12M47/06 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/502761 ,  B01L2300/0816 ,  C12N1/066 ,  C12N13/00

摘要： A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

摘要翻译： 提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。 根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在 细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠将振动有效地转移到细胞中，通过疏水处理芯片的内表面和细胞来解决粗糙表面上的微流体问题 裂解芯片可应用于LOC。

公开(公告)号：US20060084165A1

公开(公告)日：2006-04-20

申请号：US11253541

申请日：2005-10-19

申请人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

发明人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

IPC分类号： C12N1/06

CPC分类号： C12M47/06 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/502761 ,  B01L2300/0816 ,  C12N1/066 ,  C12N13/00

摘要： A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

摘要翻译： 提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。 根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在 细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠有效地将振动传递到细胞，粗糙表面上的微流体问题通过疏水处理芯片的内表面和电池来解决 裂解芯片可应用于LOC。

公开(公告)号：US20060118417A1

公开(公告)日：2006-06-08

申请号：US11281226

申请日：2005-11-16

申请人： Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

发明人： Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

IPC分类号： C07H21/04

CPC分类号： C07H21/04

摘要： Provided is a method of purifying nucleic acids using hydrogen bonding and an electric field, including: bringing a sample containing target nucleic acids into contact with an electrode coated with a material capable of forming hydrogen bonds with the target nucleic acids; applying a positive voltage to the electrode to move the target nucleic acids closer to the electrode so as to form hydrogen bonds with the material on the electrode; washing the electrode; and applying to the electrode a negative voltage to elute the bound target nucleic acids. According to the method, selectivity to nucleic acids and proteins increases due to hydrogen bonding, nucleic acid purification is possible within a short time through an electric field, and the bound nucleic acids can be efficiently eluted.

摘要翻译： 提供了使用氢键和电场来净化核酸的方法，包括：使含有靶核酸的样品与涂覆有能够与靶核酸形成氢键的材料接触; 向电极施加正电压以使靶核酸更靠近电极以与电极上的材料形成氢键; 清洗电极; 并向电极施加负电压以洗脱结合的靶核酸。 根据该方法，由于氢键，核酸和蛋白质的选择性增加，核酸纯化可以在短时间内通过电场进行，并且结合的核酸可以被有效地洗脱。

公开(公告)号：US20060110725A1

公开(公告)日：2006-05-25

申请号：US11190169

申请日：2005-07-26

申请人： Jeong-gun Lee ,  Young-nam Kwon ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Young-a Kim

发明人： Jeong-gun Lee ,  Young-nam Kwon ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Young-a Kim

IPC分类号： C12Q1/70 ,  C12Q1/68 ,  C12N7/02 ,  C12N1/08 ,  C12M1/34

CPC分类号： B01L3/502753 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487

摘要： An apparatus for and method of purifying nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples, magnetic beads, and a solid support are introduced; a vibrator attached to the capillary and mixing the samples, magnetic beads, and solid support in the capillary; a laser generator attached to the capillary and irradiating a laser beam onto the capillary; a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall; a waste chamber attached to the capillary and discharging a lysate; an elution buffer chamber attached to the capillary and eluting nucleic acids from the solid support having nucleic acids bound thereto; and a neutralization buffer chamber attached to the capillary and supplying a neutralization buffer for neutralizing the eluted nucleic acid solution. According to the apparatus and method, PCR inhibitors can be removed to increase PCR yield and nucleic acids can be purified using a silicon substrate or silica beads. Thus, the apparatus and method can be applied to LOC fabrication.

摘要翻译： 提供了一种纯化细胞或病毒核酸的装置和方法。 核酸纯化装置包括：细胞裂解毛细管，其具有样品入口，通过所述样品入口引入样品，磁珠和固体支持物; 连接到毛细管并将样品，磁珠和固体支持物混合在毛细管中的振动器; 激光发生器附接到毛细管并将激光束照射到毛细管上; 附着到毛细管并将磁珠固定到毛细管壁的磁力发生器; 连接到毛细管并排出裂解物的废物室; 洗脱缓冲室连接到毛细管并从具有与其结合的核酸的固体支持物洗脱核酸; 以及中和缓冲室，其连接到毛细管并提供用于中和洗脱的核酸溶液的中和缓冲液。 根据该装置和方法，可以除去PCR抑制剂以增加PCR产量，并且可以使用硅底物或二氧化硅珠纯化核酸。 因此，该装置和方法可以应用于LOC制造。

公开(公告)号：US07429470B2

公开(公告)日：2008-09-30

申请号：US11265461

申请日：2005-11-02

申请人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim

发明人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim

IPC分类号： C12N13/00 ,  C12N1/08 ,  G01N33/00 ,  C07H21/02

CPC分类号： B01L3/502761 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0838 ,  B01L2300/087 ,  B01L2400/0633 ,  G01N35/0098 ,  Y10T436/143333

摘要： An apparatus and method for purification of nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary; a laser generator attached to the capillary and supplying a laser to the capillary; and a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall. According to the method and apparatus, PCR yield can be increased since PCR inhibitors can be readily removed by means of a phase separation in a capillary. The use of an electromagnet ensures the removal of the PCR inhibitors. In addition, since cell lysis and DNA purification process can be simultaneously performed, LOC steps can be reduced.

摘要翻译： 提供了用于纯化细胞或病毒核酸的装置和方法。 核酸纯化装置包括：细胞裂解毛细管，其具有样品和磁珠引入的样品入口; 连接到毛细管并将样品和磁珠混合在毛细管中的振动器; 激光发生器，附接到毛细管并向毛细管提供激光; 以及附接到毛细管并将磁珠固定到毛细管壁的磁力发生器。 根据该方法和装置，可以增加PCR产量，因为通过毛细管中的相分离可以容易地除去PCR抑制剂。 使用电磁铁确保去除PCR抑制剂。 此外，由于可以同时进行细胞裂解和DNA纯化过程，因此可以减少LOC步骤。

公开(公告)号：US20060094051A1

公开(公告)日：2006-05-04

申请号：US11265461

申请日：2005-11-02

申请人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim

发明人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim

IPC分类号： C12Q1/68 ,  C12N1/08 ,  C12M1/34

CPC分类号： B01L3/502761 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0838 ,  B01L2300/087 ,  B01L2400/0633 ,  G01N35/0098 ,  Y10T436/143333

摘要： An apparatus and method for purification of nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary; a laser generator attached to the capillary and supplying a laser to the capillary; and a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall. According to the method and apparatus, PCR yield can be increased since PCR inhibitors can be readily removed by means of a phase separation in a capillary. The use of an electromagnet ensures the removal of the PCR inhibitors. In addition, since cell lysis and DNA purification process can be simultaneously performed, LOC steps can be reduced.

摘要翻译： 提供了用于纯化细胞或病毒核酸的装置和方法。 核酸纯化装置包括：细胞裂解毛细管，其具有样品和磁珠引入的样品入口; 连接到毛细管并将样品和磁珠混合在毛细管中的振动器; 激光发生器附接到毛细管并向毛细管提供激光; 以及附接到毛细管并将磁珠固定到毛细管壁的磁力发生器。 根据该方法和装置，可以增加PCR产量，因为通过毛细管中的相分离可以容易地除去PCR抑制剂。 使用电磁铁确保去除PCR抑制剂。 此外，由于可以同时进行细胞裂解和DNA纯化过程，因此可以减少LOC步骤。

公开(公告)号：US20060264620A1

公开(公告)日：2006-11-23

申请号：US11345174

申请日：2006-02-01

申请人： Myo-yong Lee ,  Joong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Yeon-ja Cho ,  Shin-i Yoo

发明人： Myo-yong Lee ,  Joong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Yeon-ja Cho ,  Shin-i Yoo

IPC分类号： C07H21/04

CPC分类号： C07H21/04 ,  C12N15/1006

摘要： Provided are methods of isolating and amplifying nucleic acids from and in a nucleic acid-containing sample. The nucleic acid isolation method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support and treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14. The nucleic acid amplification method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support; treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14; and adding a nucleic acid amplification solution to the resultant solution after the alkaline solution treatment to perform nucleic acid amplification.

摘要翻译： 提供从含核酸样品中分离和扩增核酸的方法。 核酸分离方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化的固体支持物，并用pH9至14的碱性溶液处理核酸捕获的固体支持物。核酸扩增 方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化固体支持物; 用pH9至14的碱性溶液处理核酸捕获的固体支持物; 并在碱溶液处理后向所得溶液中加入核酸扩增溶液进行核酸扩增。

公开(公告)号：US07785787B2

公开(公告)日：2010-08-31

申请号：US11345174

申请日：2006-02-01

申请人： Myo-yong Lee ,  Joong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Yeon-ja Cho ,  Shin-i Yoo

发明人： Myo-yong Lee ,  Joong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Yeon-ja Cho ,  Shin-i Yoo

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C07H21/04 ,  C12N15/1006

摘要： Provided are methods of isolating and amplifying nucleic acids from and in a nucleic acid-containing sample. The nucleic acid isolation method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support and treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14. The nucleic acid amplification method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support; treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14; and adding a nucleic acid amplification solution to the resultant solution after the alkaline solution treatment to perform nucleic acid amplification.

摘要翻译： 提供从含核酸样品中分离和扩增核酸的方法。 核酸分离方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化的固体支持物，并用pH9至14的碱性溶液处理核酸捕获的固体支持物。核酸扩增 方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化固体支持物; 用pH9至14的碱性溶液处理核酸捕获的固体支持物; 并在碱溶液处理后向所得溶液中加入核酸扩增溶液进行核酸扩增。

公开(公告)号：US07926910B2

公开(公告)日：2011-04-19

申请号：US11764844

申请日：2007-06-19

申请人： Tae-woon Cha ,  Young-nam Kwon ,  Jae-chang Lee

发明人： Tae-woon Cha ,  Young-nam Kwon ,  Jae-chang Lee

IPC分类号： B41J2/135

CPC分类号： B41J2/1606 ,  B41J2/162 ,  B41J2/164

摘要： A nozzle plate for an inkjet head and a method of manufacturing the nozzle plate includes a silicon substrate having a nozzle, a thermally oxidized silicon layer formed on an outer surface of the silicon substrate and an inner wall of the nozzle, an adhesion layer deposited on the thermally oxidized silicon layer formed on the outer surface of the silicon substrate and formed of silicon oxide, and an ink-repellent coating layer deposited on the adhesion layer.

摘要翻译： 用于喷墨头的喷嘴板和喷嘴板的制造方法包括具有喷嘴的硅基板，形成在硅基板的外表面上的热氧化硅层和喷嘴的内壁，粘合层沉积在 形成在硅衬底的外表面上并由氧化硅形成的热氧化硅层和沉积在粘合层上的拒墨涂层。

公开(公告)号：US08114467B2

公开(公告)日：2012-02-14

申请号：US12178591

申请日：2008-07-23

申请人： Won-cheol Jung ,  Bum-jae Lee ,  Jong-seop Lee ,  Young-nam Kwon ,  Tae-jung Kim

发明人： Won-cheol Jung ,  Bum-jae Lee ,  Jong-seop Lee ,  Young-nam Kwon ,  Tae-jung Kim

IPC分类号： C08F214/18

CPC分类号： C09D125/08 ,  C09D125/10

摘要： Provided are hydrophobic layer formed by polymer represented by Formula 1 below and has a weight average molecular weight of about 10,000 to about 500,000 g/mol, hydrophobic layer including the polymer and an electronic articles including the hydrophobic layer: wherein Q1, and Q2 are independently linear or branched C1-C10 alkylene groups, R1 and R2 are independently ether and/or ester containing substituents, R3 to R7 are independently selected from a hydrogen atom, a halogen atom, a cyano group, an amino group, a C1-C10 alkyl group and a C6-C12 aryl group; and the ratio of a:b+c is from 10:1 to 1:1,000. A coated electronic article comprising the hydrophobic layer and a method of forming the coated electronic article are also provided.

摘要翻译： 提供由以下通式1表示的聚合物形成的疏水层，其重均分子量为约10,000至约500,000g / mol，包含聚合物的疏水层和包含疏水层的电子制品：其中Q1和Q2独立地为 直链或支链的C1-C10亚烷基，R1和R2独立地是醚和/或含酯取代基，R3至R7独立地选自氢原子，卤素原子，氰基，氨基，C1-C10烷基 基团和C 6 -C 12芳基; a：b + c的比例为10:1〜1:1000。 还提供了包括疏水层的涂覆的电子制品和形成涂覆的电子制品的方法。