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1.发明授权Lipolytic enzyme variant with improved stability and polynucleotides encoding same 有权具有改善稳定性的脂肪分解酶变体和编码相同的多核苷酸公开(公告)号：US09163203B2
公开(公告)日：2015-10-20
申请号：US12393512
申请日：2009-02-26
申请人： Jesper Vind , Kim Borch , Allan Svendsen , Robert Van der Lans , Lise Munch Mikkelsen , Christian Isak Jorgensen , Shamkant Anant Patkar
发明人： Jesper Vind , Kim Borch , Allan Svendsen , Robert Van der Lans , Lise Munch Mikkelsen , Christian Isak Jorgensen , Shamkant Anant Patkar
CPC分类号： C11D3/38627 , C12N9/20 , C12N15/00
摘要： The invention provides lipolytic enzyme variants having improved in-detergent stability and polynucleotides encoding same. Lipolytic enzyme variants with improved in-detergent stability are obtained by substituting certain specified amino acid residues in a parent lipolytic enzyme.
摘要翻译： 本发明提供具有改进的洗涤剂稳定性的脂肪分解酶变体和编码它的多核苷酸。 通过用母体脂肪分解酶中的某些特定的氨基酸残基取代具有改善的洗涤剂稳定性的脂肪分解酶变体。
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公开(公告)号：US20090217464A1
公开(公告)日：2009-09-03
申请号：US12393136
申请日：2009-02-26
申请人： Philip Frank Souter , Neil Joseph Lant , Therese Clare Haynes , Jesper Vind , Kim Borch , Allan Svendsen , Robert Van der Lans , Lise Munch Mikkelsen , Christian Isak Jorgensen , Shamkant Anant Patkar
发明人： Philip Frank Souter , Neil Joseph Lant , Therese Clare Haynes , Jesper Vind , Kim Borch , Allan Svendsen , Robert Van der Lans , Lise Munch Mikkelsen , Christian Isak Jorgensen , Shamkant Anant Patkar
CPC分类号： C12N9/20 , C11D1/44 , C11D3/3788 , C11D3/38627
摘要： The invention provides detergent compositions comprising lipolytic enzyme variants having improved in-detergent stability. Lipolytic enzyme variants with improved in-detergent stability are obtained by substituting certain specified amino acid residues in a parent lipolytic enzyme.
摘要翻译： 本发明提供了包含具有改善的洗涤剂稳定性的脂肪分解酶变体的洗涤剂组合物。 通过用母体脂肪分解酶中的某些特定的氨基酸残基取代具有改善的洗涤剂稳定性的脂肪分解酶变体。
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3.发明申请Lipolytic Enzyme Variant With Improved Stability And Polynucleotides Encoding Same 有权脂肪分解酶变体与改进的稳定性和多核苷酸编码相同公开(公告)号：US20090221034A1
公开(公告)日：2009-09-03
申请号：US12393512
申请日：2009-02-26
申请人： Jesper Vind , Kim Borch , Allan Svendsen , Robert Van der Lans , Lise Munch Mikkelsen , Christian Isak Jorgensen , Shamkant Anant Patkar
发明人： Jesper Vind , Kim Borch , Allan Svendsen , Robert Van der Lans , Lise Munch Mikkelsen , Christian Isak Jorgensen , Shamkant Anant Patkar
CPC分类号： C11D3/38627 , C12N9/20 , C12N15/00
摘要： The invention provides lipolytic enzyme variants having improved in-detergent stability and polynucleotides encoding same. Lipolytic enzyme variants with improved in-detergent stability are obtained by substituting certain specified amino acid residues in a parent lipolytic enzyme.
摘要翻译： 本发明提供具有改进的洗涤剂稳定性的脂肪分解酶变体和编码它的多核苷酸。 通过用母体脂肪分解酶中的某些特定的氨基酸残基取代具有改善的洗涤剂稳定性的脂肪分解酶变体。
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公开(公告)号：US10577568B2
公开(公告)日：2020-03-03
申请号：US13124089
申请日：2009-11-11
申请人： Lone Kierstein Nielsen , Lise Munch Mikkelsen , Esben Peter Friis , Juergen Carsten Franz Knoetzel , Ole Simonsen
发明人： Lone Kierstein Nielsen , Lise Munch Mikkelsen , Esben Peter Friis , Juergen Carsten Franz Knoetzel , Ole Simonsen
摘要： Peptide aldehydes containing a ureido group are effective as stabilizers for protease in a detergent composition, and as stabilizers for a second enzyme in a liquid composition with a protease.
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公开(公告)号：US20140336098A1
公开(公告)日：2014-11-13
申请号：US14129748
申请日：2012-06-29
IPC分类号： C11D3/386
CPC分类号： C11D3/38663 , C07K5/06026 , C07K5/06043 , C07K5/06052 , C07K5/06078 , C07K5/06095 , C07K5/0808 , C07K5/0812 , C11D3/38618 , Y02P20/55
摘要： The conversion of a peptide aldehyde to a hydrosulfite adduct can be used to increase the aqueous solubility in the purification of the peptide aldehydes. Advantageously, this hydrosulfite adduct is itself effective as a subtilisin inhibitor and stabilizer and it can also stabilize a second enzyme if present. The hydrosulfite adduct is effective as a subtilisin inhibitor, and it maintains its inhibitory and stabilizing effect in a liquid detergent during storage. Thus, use of the hydrosulfite adduct can avoid the cost and time of converting it back to the peptide aldehyde and subsequent drying of the peptide aldehyde can be saved, and this can avoid the inconvenience of handling the peptide aldehyde in powder form or as a highly diluted aqueous solution.
摘要翻译： 可以使用肽醛向连二亚硫酸酯加合物的转化来提高肽醛纯化中的水溶性。 有利地，该连二亚硫酸酯加合物本身作为枯草杆菌蛋白酶抑制剂和稳定剂本身是有效的，并且如果存在，它也可以稳定第二种酶。 连二亚硫酸盐加合物作为枯草杆菌蛋白酶抑制剂是有效的，并且在储存期间它在液体洗涤剂中保持其抑制和稳定作用。 因此，使用连二亚硫酸盐加合物可以避免将其转化为肽醛的成本和时间，并且可以节省随后的肽醛的干燥，并且这可以避免处理粉末形式的肽醛或高度的 稀释水溶液。
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公开(公告)号：US20120149625A1
公开(公告)日：2012-06-14
申请号：US13391832
申请日：2010-09-21
IPC分类号： C11D3/386
CPC分类号： C11D3/38609 , C11D3/38663
摘要： The addition of a protease inhibitor to a particulate protease-containing detergent composition can improve its detergency and the inhibitor can improve the in-wash stability of the protease in a solution of the detergent.
摘要翻译： 向含微粒蛋白酶的洗涤剂组合物中加入蛋白酶抑制剂可以改善其去污力，并且抑制剂可以改善洗涤剂溶液中蛋白酶的洗涤间稳定性。
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公开(公告)号：US20110212877A1
公开(公告)日：2011-09-01
申请号：US13124089
申请日：2009-11-11
申请人： Lone Kierstein Nielsen , Lise Munch Mikkelsen , Esben Peter Friis , Juergen Carsten Franz Knoetzel , Ole Simonsen
发明人： Lone Kierstein Nielsen , Lise Munch Mikkelsen , Esben Peter Friis , Juergen Carsten Franz Knoetzel , Ole Simonsen
摘要： Peptide aldehydes containing a ureido group are effective as stabilizers for protease in a detergent composition, and as stabilizers for a second enzyme in a liquid composition with a protease.
摘要翻译： 含有脲基的肽醛在洗涤剂组合物中作为蛋白酶的稳定剂，作为具有蛋白酶的液体组合物中的第二种酶的稳定剂是有效的。
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公开(公告)号：US09085750B2
公开(公告)日：2015-07-21
申请号：US14129748
申请日：2012-06-29
CPC分类号： C11D3/38663 , C07K5/06026 , C07K5/06043 , C07K5/06052 , C07K5/06078 , C07K5/06095 , C07K5/0808 , C07K5/0812 , C11D3/38618 , Y02P20/55
摘要： The conversion of a peptide aldehyde to a hydrosulfite adduct can be used to increase the aqueous solubility in the purification of the peptide aldehydes. Advantageously, this hydrosulfite adduct is itself effective as a subtilisin inhibitor and stabilizer and it can also stabilize a second enzyme if present. The hydrosulfite adduct is effective as a subtilisin inhibitor, and it maintains its inhibitory and stabilizing effect in a liquid detergent during storage. Thus, use of the hydrosulfite adduct can avoid the cost and time of converting it back to the peptide aldehyde and subsequent drying of the peptide aldehyde can be saved, and this can avoid the inconvenience of handling the peptide aldehyde in powder form or as a highly diluted aqueous solution.
摘要翻译： 可以使用肽醛向连二亚硫酸酯加合物的转化来提高肽醛纯化中的水溶性。 有利地，该连二亚硫酸酯加合物本身作为枯草杆菌蛋白酶抑制剂和稳定剂本身是有效的，并且如果存在，它也可以稳定第二种酶。 连二亚硫酸盐加合物作为枯草杆菌蛋白酶抑制剂是有效的，并且在储存期间它在液体洗涤剂中保持其抑制和稳定作用。 因此，使用连二亚硫酸盐加合物可以避免将其转化为肽醛的成本和时间，并且可以节省随后的肽醛的干燥，并且这可以避免处理粉末形式的肽醛或高度的 稀释水溶液。
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公开(公告)号：US20110039752A1
公开(公告)日：2011-02-17
申请号：US12933895
申请日：2009-03-26
申请人： Lone Kierstein Nielsen , Lise Munch Mikkelsen , Esben Peter Friis , Juergen Carsten Franz Knoetzel , Ole Simonsen , Lotte Rugholm Soerensen
发明人： Lone Kierstein Nielsen , Lise Munch Mikkelsen , Esben Peter Friis , Juergen Carsten Franz Knoetzel , Ole Simonsen , Lotte Rugholm Soerensen
CPC分类号： C07K5/0808 , C07K2/00 , C07K5/0806 , C07K5/0817 , C07K5/101 , C07K5/1016 , C07K5/1024 , C11D3/38663 , C12N9/54 , C12N9/96 , Y02P20/55
摘要： Peptide aldehydes or ketone derivatives are particularly efficient for stabilizing subtilisin-type proteases in aqueous compositions such as liquid detergents, including peptide compounds with OH-substituted phenylalanine aldehyde as the C-terminal residue.
摘要翻译： 肽醛或酮衍生物在水性组合物如液体洗涤剂中稳定枯草杆菌蛋白酶型蛋白酶特别有效，包括具有OH取代的苯丙氨酸醛作为C末端残基的肽化合物。
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公开(公告)号：US09181296B2
公开(公告)日：2015-11-10
申请号：US12933895
申请日：2009-03-26
申请人： Lone Kierstein Nielsen , Lise Munch Mikkelsen , Esben Peter Friis , Juergen Carsten Franz Knoetzel , Ole Simonsen , Lotte Rugholm Soerensen
发明人： Lone Kierstein Nielsen , Lise Munch Mikkelsen , Esben Peter Friis , Juergen Carsten Franz Knoetzel , Ole Simonsen , Lotte Rugholm Soerensen
IPC分类号： C07K5/08 , C07K5/10 , C07K2/00 , C07K5/083 , C07K5/09 , C07K5/103 , C07K5/107 , C07K5/117 , C11D3/386 , C12N9/54 , C12N9/96
CPC分类号： C07K5/0808 , C07K2/00 , C07K5/0806 , C07K5/0817 , C07K5/101 , C07K5/1016 , C07K5/1024 , C11D3/38663 , C12N9/54 , C12N9/96 , Y02P20/55
摘要： Peptide aldehydes or ketone derivatives are particularly efficient for stabilizing subtilisin-type proteases in aqueous compositions such as liquid detergents, including peptide compounds with OH-substituted phenylalanine aldehyde as the C-terminal residue.
摘要翻译： 肽醛或酮衍生物在水性组合物如液体洗涤剂中稳定枯草杆菌蛋白酶型蛋白酶特别有效，包括具有OH取代的苯丙氨酸醛作为C末端残基的肽化合物。
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