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    Processes and compositions for the isolation of human relaxin
    10.
    发明授权
    Processes and compositions for the isolation of human relaxin 失效
    用于分离人类松弛素的方法和组合物

    公开(公告)号：US5464756A

    公开(公告)日：1995-11-07

    申请号：US908766

    申请日：1992-07-01

    申请人： Dennis J. Henner ,  Richard L. Vandlen ,  James A. Wilkins ,  Daniel G. Yansura

    发明人： Dennis J. Henner ,  Richard L. Vandlen ,  James A. Wilkins ,  Daniel G. Yansura

    IPC分类号： A61K38/00 ,  A61P13/02 ,  A61P15/00 ,  C07H21/04 ,  C07K1/113 ,  C07K1/12 ,  C07K14/00 ,  C07K14/575 ,  C07K14/64 ,  C12N1/21 ,  C12N15/09 ,  C12N15/12 ,  C12N15/16 ,  C12P21/00 ,  C12P21/02 ,  C12P21/06 ,  C12R1/19 ,  C12N15/70

    CPC分类号： C07K14/64 ,  Y10S435/849 ,  Y10S930/31

    摘要： A process is provided for cleaving a polypeptide into at least two polypeptide components comprising treating a reduced, free-cysteine form of the polypeptide with a cleaving agent under conditions for cleaving the polypeptide at a desired junction between the polypeptide cleavage products. More preferably, the process for cleaving comprises culturing cells containing DNA encoding said polypeptide, wherein at least one Asp codon is present in said DNA at a desired junction between the components to be cleaved from each other, said culturing resulting in expression of the DNA to produce the polypeptide in the host cell culture; and treating a reduced, free-cysteine form of the polypeptide with dilute acid under conditions for cleaving the polypeptide at the Asp junction. In particular embodiments, a DNA sequence is provided that encodes a relaxin precursor and includes codons encoding aspartic acid-containing linkers at novel positions within the precursor, allowing the ready cleavage of relaxin A peptides by treatment with dilute acid.

    摘要翻译： 提供了一种用于将多肽切割成至少两种多肽组分的方法，包括在多肽切割产物之间的所需连接处切割多肽的条件下用切割剂处理还原的游离半胱氨酸形式的多肽。 更优选地，所述切割方法包括培养含有编码所述多肽的DNA的细胞，其中至少一个Asp密码子存在于所述DNA中在待切割的组分之间的所需连接处，所述培养导致DNA的表达 在宿主细胞培养物中产生多肽; 以及在Asp连接处切割多肽的条件下用稀酸处理还原的游离半胱氨酸形式的多肽。 在具体实施方案中，提供了编码松弛素前体的DNA序列，并且包括在前体内的新位置编码含有天冬氨酸的接头的密码子，从而允许通过用稀酸处理来快速切割松弛素A肽。