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公开(公告)号：US07038026B2
公开(公告)日：2006-05-02
申请号：US10275071
申请日：2001-05-25
CPC分类号： C12N15/1006 , C12Q1/6839
摘要： Method for double-stranded DNA purification, by which a solution containing said DNA in a mixture with other components is passed over a support on which is covalently coupled in oligonucleotide capable of hybridizing with a specific sequence present on said DNA to form a triple helix.
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2.发明授权公开(公告)号：US08017744B2
公开(公告)日：2011-09-13
申请号：US12478418
申请日：2009-06-04
IPC分类号： C07H21/02
CPC分类号： C12N15/1006 , C12Q1/6839
摘要： A method for double-stranded DNA purification, by which a solution containing DNA in a mixture with other components is passed over a support on which is covalently coupled an oligonucleotide capable of hybridizing with a specific sequence present on the DNA to form a triple helix.
摘要翻译： 一种用于双链DNA纯化的方法，其中将含有与其它组分的混合物中的DNA的溶液通过其上共价偶联能够与存在于DNA上的特定序列杂交的寡核苷酸以形成三螺旋的载体。
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公开(公告)号：US06319672B1
公开(公告)日：2001-11-20
申请号：US09580923
申请日：2000-05-26
IPC分类号： C12Q168
CPC分类号： C12Q1/6839 , Y10S977/924 , C12Q2565/137
摘要： Method for double-stranded DNA purification, by which a solution containing said DNA in a mixture with other components is passed over a support on which is covalently coupled in oligonucleotide capable of hybridizing with a specific sequence present on said DNA to form a triple helix.
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4.发明授权公开(公告)号：US08399636B2
公开(公告)日：2013-03-19
申请号：US13205032
申请日：2011-08-08
CPC分类号： C12N15/1006 , C12Q1/6839
摘要： A method for double-stranded DNA purification, by which a solution containing DNA in a mixture with other components is passed over a support on which is covalently coupled an oligonucleotide capable of hybridizing with a specific sequence present on the DNA to form a triple helix.
摘要翻译： 一种用于双链DNA纯化的方法，其中将含有与其它组分的混合物中的DNA的溶液通过其上共价偶联能够与存在于DNA上的特定序列杂交的寡核苷酸以形成三螺旋的载体。
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5.发明申请公开(公告)号：US20110319605A1
公开(公告)日：2011-12-29
申请号：US13205032
申请日：2011-08-08
IPC分类号： C07H21/04
CPC分类号： C12N15/1006 , C12Q1/6839
摘要： Method for double-stranded DNA purification, by which a solution containing said DNA in a mixture with other components is passed over a support on which is covalently coupled in oligonucleotide capable of hybridizing with a specific sequence present on said DNA to form a triple helix.
摘要翻译： 用于双链DNA纯化的方法，其中将含有与其它组分的混合物中的所述DNA的溶液通过载体，其上共价偶联于寡核苷酸中，所述寡核苷酸能够与存在于所述DNA上的特定序列杂交以形成三螺旋。
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6.发明申请公开(公告)号：US20100010208A1
公开(公告)日：2010-01-14
申请号：US12478418
申请日：2009-06-04
IPC分类号： C07H21/04
CPC分类号： C12N15/1006 , C12Q1/6839
摘要： Method for double-stranded DNA purification, by which a solution containing said DNA in a mixture with other components is passed over a support on which is covalently coupled in oligonucleotide capable of hybridizing with a specific sequence present on said DNA to form a triple helix.
摘要翻译： 用于双链DNA纯化的方法，其中将含有与其它组分的混合物中的所述DNA的溶液通过载体，其上共价偶联于寡核苷酸中，所述寡核苷酸能够与存在于所述DNA上的特定序列杂交以形成三螺旋。
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公开(公告)号：US06492164B1
公开(公告)日：2002-12-10
申请号：US09655728
申请日：2000-09-05
IPC分类号： C12N1564
CPC分类号： C12N15/10 , C12N15/85 , C12N2800/30 , C12N2830/002 , C12N2830/55 , C12Q1/6813 , C12Q1/6839
摘要： Double-stranded DNA molecules characterized in that they are circular and in that they essentially include one or more genes of interest.
摘要翻译： 双链DNA分子的特征在于它们是环状的，并且其基本上包括一个或多个感兴趣的基因。
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8.发明授权公开(公告)号：US06649394B1
公开(公告)日：2003-11-18
申请号：US09398184
申请日：1999-09-17
IPC分类号： C12N990
摘要： A novel topoisomerase IV, nucleotide sequences coding for said enzyme, corresponding vectors, and the use of said enzyme for screening biologically active materials.
摘要翻译： 新的拓扑异构酶IV，编码所述酶的核苷酸序列，相应的载体，以及所述酶用于筛选生物活性物质的用途。
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公开(公告)号：US06410273B1
公开(公告)日：2002-06-25
申请号：US09202314
申请日：1998-12-11
申请人： Joël Crouzet , Béatrice Cameron
发明人： Joël Crouzet , Béatrice Cameron
IPC分类号： C12N1500
CPC分类号： C12N9/1007 , A61K48/00 , C12N15/1003
摘要： The invention discloses the preparation of DNA, in particular plasmid DNA. More particularly it concerns the production of bacterial plasmid DNA to be used in gene therapy, in the form of plasmid, minicircle supercoiled, loose or linear.
摘要翻译： 本发明公开了DNA的制备，特别是质粒DNA。 更具体地说，涉及用于基因治疗的细菌质粒DNA的生产，质粒的形式，小环状超螺旋的，松散的或线性的。
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10.发明授权Variants of thymidine kinase, nucleic acids encoding them, and methods of using them 失效胸苷激酶的变体，编码它们的核酸，以及使用它们的方法公开(公告)号：US06207150B1
公开(公告)日：2001-03-27
申请号：US09125099
申请日：1998-08-06
申请人： Joël Crouzet , Francis Blanche , Michel Couder , Béatrice Cameron
发明人： Joël Crouzet , Francis Blanche , Michel Couder , Béatrice Cameron
IPC分类号： A61K3851
CPC分类号： C12N9/1211 , A01K2217/05 , A61K38/00
摘要： The present invention relates to a nucleic acid sequence characterized in that it is derived from the wild nucleic acid sequence coding for a thymidine kinase, said nucleic acid sequence having at least one mutation in the region corresponding to the ATP binding site and conveniently a second mutation in the N-terminal region and/or C-terminal region. It also relates to variants of the wild thymidine kinase and their use in genic therapy.
摘要翻译： 本发明涉及一种核酸序列，其特征在于其衍生自编码胸苷激酶的野生型核酸序列，所述核酸序列在对应于ATP结合位点的区域中具有至少一个突变，并且方便地具有第二突变 在N-末端区域和/或C-末端区域。 它还涉及野生胸苷激酶的变体及其在基因治疗中的用途。
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