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31 条记录 (耗时 0.034 秒)

    Polyvalent display libraries
    7.
    发明授权
    Polyvalent display libraries 失效

    公开(公告)号：US06986986B1

    公开(公告)日：2006-01-17

    申请号：US09563055

    申请日：2000-05-01

    申请人： Joe Buechler ,  Gunars Valkirs ,  Jeff Gray

    发明人： Joe Buechler ,  Gunars Valkirs ,  Jeff Gray

    IPC分类号： C12Q1/68 ,  C12Q1/70

    CPC分类号： C40B40/02 ,  C07K16/00 ,  C07K2319/00 ,  C12N15/1037

    摘要： The invention is directed to inter alia two related but self-sufficient improvements in conventional display methods. The first improvement provides methods of enriching conventional display libraries for members displaying more than one copy of a polypeptide prior to affinity screening of such libraries with a target of interest. These methods can achieve diverse populations in which the vast majority of members retaining full-length coding sequences encode polypeptides having specific affinity for the target. In a second aspect, the invention provides methods of subcloning nucleic acids encoding displayed polypeptides of enriched libraries from a display vector to an expression vector without the need for clonal isolation of individual members. These methods result in polyclonal libraries of antibodies and other polypeptides for use, e.g., as diagnostic or therapeutic reagents.

    Chimeric polyclonal antibodies
    8.
    发明授权
    Chimeric polyclonal antibodies 有权
    嵌合多克隆抗体

    公开(公告)号：US06420113B1

    公开(公告)日：2002-07-16

    申请号：US09410903

    申请日：1999-10-02

    申请人： Joe Buechler ,  Gunars Valkirs ,  Jeff Gray

    发明人： Joe Buechler ,  Gunars Valkirs ,  Jeff Gray

    IPC分类号： C12Q168

    CPC分类号： C40B40/02 ,  C07K16/00 ,  C07K16/1282 ,  C07K2319/00 ,  C12N15/1037 ,  G01N33/54326 ,  G01N2333/33 ,  G01N2400/50

    摘要： The invention is directed to production of chimeric antibodies using display screening methods. The invention is based in part on two related but self-sufficient improvements in conventional display methods. The first improvement provides methods of enriching conventional display libraries for members displaying more than one copy of a polypeptide prior to affinity screening of such libraries with a target of interest. These methods can achieve diverse populations in which the vast majority of members retaining full-length coding sequences encode polypeptides having specific affinity for the target. In a second aspect, the invention provides methods of subcloning nucleic acids encoding displayed polypeptides of enriched libraries from a display vector to an expression vector without the need for clonal isolation of individual members. These methods can be used to produce polyclonal libraries of chimeric antibodies for use, e.g., as diagnostic or therapeutic reagents.

    摘要翻译： 本发明涉及使用显示筛选方法生产嵌合抗体。 本发明部分地基于传统显示方法的两个相关但是自足的改进。 第一个改进提供了丰富常规显示库的方法，用于在用感兴趣的靶标亲和筛选此类文库之前，显示多于一个拷贝的成员的成员。 这些方法可以实现多种群，其中保留全长编码序列的绝大多数成员编码对靶具有特异性亲和力的多肽。 在第二方面，本发明提供将编码显示多肽的多肽的核酸从显示载体亚克隆到表达载体的方法，而不需要单个成员的克隆分离。 这些方法可用于制备用于例如诊断或治疗试剂的嵌合抗体多克隆文库。

    Polyvalent display libraries
    9.
    发明授权
    Polyvalent display libraries 失效
    多价显示库

    公开(公告)号：US6057098A

    公开(公告)日：2000-05-02

    申请号：US832985

    申请日：1997-04-04

    申请人： Joe Buechler ,  Gunars Valkirs ,  Jeff Gray

    发明人： Joe Buechler ,  Gunars Valkirs ,  Jeff Gray

    IPC分类号： C07K16/00 ,  C12N15/10 ,  C12P21/04 ,  C40B40/02 ,  C12Q1/68 ,  C12N15/64 ,  G01N33/53

    CPC分类号： C40B40/02 ,  C07K16/00 ,  C12N15/1037 ,  C07K2319/00

    摘要： The invention is directed to inter alia two related but self-sufficient improvements in conventional display methods. The first improvement provides methods of enriching conventional display libraries for members displaying more than one copy of a polypeptide prior to affinity screening of such libraries with a target of interest. These methods can achieve diverse populations in which the vast majority of members retaining full-length coding sequences encode polypeptides having specific affinity for the target. In a second aspect, the invention provides methods of subcloning nucleic acids encoding displayed polypeptides of enriched libraries from a display vector to an expression vector without the need for clonal isolation of individual members. These methods result in polyclonal libraries of antibodies and other polypeptides for use, e.g., as diagnostic or therapeutic reagents.

    摘要翻译： 本发明特别涉及传统显示方法中的两个相关但自给自足的改进。 第一个改进提供了丰富常规显示库的方法，用于在用感兴趣的靶标亲和筛选此类文库之前，显示多于一个拷贝的成员的成员。 这些方法可以实现多种群，其中保留全长编码序列的绝大多数成员编码对靶具有特异性亲和力的多肽。 在第二方面，本发明提供将编码显示多肽的多肽的核酸从显示载体亚克隆到表达载体的方法，而不需要单个成员的克隆分离。 这些方法导致抗体和其他多肽的多克隆文库用于例如诊断或治疗试剂。

    Polyclonal libraries
    10.
    发明授权
    Polyclonal libraries 失效
    多克隆文库

    公开(公告)号：US06555310B1

    公开(公告)日：2003-04-29

    申请号：US08835159

    申请日：1997-04-04

    申请人： Jeff Gray ,  Joe Buechler ,  Gunars Valkirs

    发明人： Jeff Gray ,  Joe Buechler ,  Gunars Valkirs

    IPC分类号： C12Q170

    CPC分类号： C40B40/02 ,  C07K16/00 ,  C07K2319/00 ,  C12N15/1037

    摘要： The invention is directed to inter alia two related but self-sufficient improvements in conventional display methods. The first improvement provides methods of enriching conventional display libraries for members displaying more than one copy of a polypeptide prior to affinity screening of such libraries with a target of interest. These methods can achieve diverse populations in which the vast majority of members retaining full-length coding sequences encode polypeptides having specific affinity for the target. In a second aspect, the invention provides methods of subcloning nucleic acids encoding displayed polypeptides of enriched libraries from a display vector to an expression vector without the need for clonal isolation of individual members. These methods result in polyclonal libraries of antibodies and other polypeptides for use, e.g., as diagnostic or therapeutic reagents.

    摘要翻译： 本发明特别涉及传统显示方法中的两个相关但自给自足的改进。 第一个改进提供了丰富常规显示库的方法，用于在用感兴趣的靶标亲和筛选此类文库之前，显示多于一个拷贝的成员的成员。 这些方法可以实现多种群，其中保留全长编码序列的绝大多数成员编码对靶具有特异性亲和力的多肽。 在第二方面，本发明提供将编码显示多肽的多肽的核酸从显示载体亚克隆到表达载体的方法，而不需要单个成员的克隆分离。 这些方法导致抗体和其他多肽的多克隆文库用于例如诊断或治疗试剂。