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    Stem cells and their use in transplantation
    1.
    发明授权
    Stem cells and their use in transplantation 有权
    干细胞及其在移植中的应用

    公开(公告)号：US07537756B2

    公开(公告)日：2009-05-26

    申请号：US11132687

    申请日：2005-05-19

    申请人： Joel E. Habener ,  Hendrik Zulewski ,  Elizabeth J. Abraham ,  Mario Vallejo ,  Denise L. Faustman ,  Melissa K. Thomas

    发明人： Joel E. Habener ,  Hendrik Zulewski ,  Elizabeth J. Abraham ,  Mario Vallejo ,  Denise L. Faustman ,  Melissa K. Thomas

    IPC分类号： A61K35/12 ,  C12N5/00

    CPC分类号： C12N5/0676 ,  A01K67/0271 ,  A61K35/12 ,  A61K2035/122 ,  C12N5/0677 ,  C12N5/0678 ,  C12N2500/34 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/335 ,  C12N2510/02

    摘要： Methods and compositions are described for the treatment of type I insulin-dependent diabetes mellitus and other conditions using newly identified stem cells that are capable of differentiation into a variety of pancreatic islet cells, including insulin-producing beta cells, as well as hepatocytes. Nestin has been identified as a molecular marker for pancreatic stem cells, while cytokeratin-19 serves as a marker for a distinct class of islet ductal cells. Methods are described whereby nestin-positive stem cells can be isolated from pancreatic islets and cultured to obtain further stem cells or pseudo-islet like structures. Methods for ex vivo differentiation of the pancreatic stem cells are disclosed. Methods are described whereby pancreatic stem cells can be isolated, expanded, and transplanted into a patient in need thereof, either allogeneically, isogeneically or xenogenically, to provide replacement for lost or damaged insulin-secreting cells or other cells.

    摘要翻译： 描述了用于治疗I型胰岛素依赖性糖尿病和其它条件的方法和组合物，其使用能够分化成多种胰岛细胞（包括产生胰岛素的β细胞）以及肝细胞的新鉴定的干细胞。 巢蛋白已被鉴定为胰腺干细胞的分子标记，而细胞角蛋白-19可作为不同类别的胰岛导管细胞的标记物。 描述了方法，其中可以从胰岛中分离巢蛋白阳性干细胞并培养以获得进一步的干细胞或假胰岛样结构。 公开了胰腺干细胞离体分化方法。 描述了方法，其中胰腺干细胞可以被分离，扩增和移植到有需要的患者中，同种异体地，异质性或异质性的，以提供丢失或损伤的胰岛素分泌细胞或其他细胞的替代。

    Stem cells and their use in transplantation
    2.
    发明授权
    Stem cells and their use in transplantation 有权
    干细胞及其在移植中的应用

    公开(公告)号：US07438902B2

    公开(公告)日：2008-10-21

    申请号：US10984645

    申请日：2004-11-09

    申请人： Joel E. Habener ,  Henryk Zulewski ,  Elizabeth J. Abraham ,  Mario Vallejo ,  Denise L. Faustman ,  Melissa K. Thomas

    发明人： Joel E. Habener ,  Henryk Zulewski ,  Elizabeth J. Abraham ,  Mario Vallejo ,  Denise L. Faustman ,  Melissa K. Thomas

    IPC分类号： A61K35/12 ,  C12N5/00

    CPC分类号： C12N5/0676 ,  A01K67/0271 ,  A61K35/12 ,  A61K2035/122 ,  C12N5/0677 ,  C12N5/0678 ,  C12N2500/34 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/335 ,  C12N2510/02

    摘要： Methods and compositions are described for the treatment of type I insulin-dependent diabetes mellitus and other conditions using newly identified stem cells that are capable of differentiation into a variety of pancreatic islet cells, including insulin-producing beta cells, as well as hepatocytes. Nestin has been identified as a molecular marker for pancreatic stem cells, while cytokeratin-19 serves as a marker for a distinct class of islet ductal cells. Methods are described whereby nestin-positive stem cells can be isolated from pancreatic islets and cultured to obtain further stem cells or pseudo-islet like structures. Methods for ex vivo differentiation of the pancreatic stem cells are disclosed. Methods are described whereby pancreatic stem cells can be isolated, expanded, and transplanted into a patient in need thereof, either allogeneically, isogeneically or xenogenically, to provide replacement for lost or damaged insulin-secreting cells or other cells.

    摘要翻译： 描述了用于治疗I型胰岛素依赖性糖尿病和其它条件的方法和组合物，其使用能够分化成多种胰岛细胞（包括产生胰岛素的β细胞）以及肝细胞的新鉴定的干细胞。 巢蛋白已被鉴定为胰腺干细胞的分子标记，而细胞角蛋白-19可作为不同类别的胰岛导管细胞的标记物。 描述了方法，其中可以从胰岛中分离巢蛋白阳性干细胞并培养以获得进一步的干细胞或假胰岛样结构。 公开了胰腺干细胞离体分化方法。 描述了方法，其中胰腺干细胞可以被分离，扩增和移植到有需要的患者中，同种异体地，异质性或异质性的，以提供丢失或损伤的胰岛素分泌细胞或其他细胞的替代。

    Blood cell sorting methods and systems
    5.
    发明授权
    Blood cell sorting methods and systems 有权
    血细胞分选方法和系统

    公开(公告)号：US07867765B2

    公开(公告)日：2011-01-11

    申请号：US11646985

    申请日：2006-12-28

    申请人： Denise L. Faustman ,  Douglas E. Burger

    发明人： Denise L. Faustman ,  Douglas E. Burger

    IPC分类号： G01N33/48 ,  G01N1/18

    CPC分类号： C12N13/00 ,  C12N5/0087 ,  C12N5/0634 ,  G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255

    摘要： The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.

    摘要翻译： 本发明涉及使用特异性结合WBC的磁性颗粒和一系列特定步骤和条件从样品例如全血分离白细胞（WBC）的方法。 所述方法可以包括以下一种或多种：在WBC分离之前降低样品的粘度，以特定频率搅拌样品，和/或使用样品容器排列成使得所有样品都放置在非常接近的位置 ，2,1或0.5mm）到磁场源。 新方法提供了高产率，纯度和生存能力的WBC制剂的分离。 这些方法旨在与自动化协议兼容，以便快速处理多个样品。

    Blood cell sorting methods and systems
    6.
    发明授权
    Blood cell sorting methods and systems 有权
    血细胞分选方法和系统

    公开(公告)号：US08753888B2

    公开(公告)日：2014-06-17

    申请号：US13481237

    申请日：2012-05-25

    申请人： Denise L. Faustman ,  Douglas E. Burger

    发明人： Denise L. Faustman ,  Douglas E. Burger

    IPC分类号： G01N33/48 ,  G01N1/18 ,  C12N13/00

    CPC分类号： C12N13/00 ,  C12N5/0087 ,  C12N5/0634 ,  G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255

    摘要： The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.

    摘要翻译： 本发明涉及使用特异性结合WBC的磁性颗粒和一系列特定步骤和条件从样品例如全血分离白细胞（WBC）的方法。 所述方法可以包括以下一种或多种：在WBC分离之前降低样品的粘度，以特定频率搅拌样品，和/或使用样品容器排列成使得所有样品都放置在非常接近的位置 ，2,1或0.5mm）到磁场源。 新方法提供了高产率，纯度和生存能力的WBC制剂的分离。 这些方法旨在与自动化协议的兼容性快速处理多个样品。

    Methods for diagnosing and treating autoimmune disease
    8.
    发明授权
    Methods for diagnosing and treating autoimmune disease 失效
    诊断和治疗自身免疫性疾病的方法

    公开(公告)号：US06617171B2

    公开(公告)日：2003-09-09

    申请号：US09031629

    申请日：1998-02-27

    申请人： Denise L. Faustman ,  Takuma Hayashi

    发明人： Denise L. Faustman ,  Takuma Hayashi

    IPC分类号： G01N33566

    CPC分类号： C07K14/4702 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4703 ,  C12N9/1205 ,  C12N9/6421 ,  C12Q1/37 ,  G01N33/564 ,  G01N2333/9121 ,  G01N2333/96425 ,  G01N2500/04 ,  G01N2800/042

    摘要： The invention provides a method of detecting autoimmune disease in a mammal, comprising providing a biological sample from a mammal and detecting proteasome activity, wherein a reduction in proteasome activity from a basal state is indicative of autoimmune disease. In addition, the invention encompasses a method of treating an autoimmune disease in a mammal, comprising administering to a mammal suspected of suffering from an autoimmune disease an agent which restores NF&kgr;B activity in an amount and for a time sufficient to result in normal NF&kgr;B activity in the mammal.

    摘要翻译： 本发明提供一种检测哺乳动物自身免疫性疾病的方法，其包括从哺乳动物提供生物样品和检测蛋白酶体活性，其中来自基础状态的蛋白酶体活性的降低指示自身免疫性疾病。 此外，本发明包括治疗哺乳动物自身免疫性疾病的方法，包括对怀疑患有自身免疫性疾病的哺乳动物施用足以导致NFκB活性正常的量的NFκB活性的药物 哺乳动物。

    METHODS FOR THE TREATMENT OF AUTOIMMUNE DISEASES
    9.
    发明申请
    METHODS FOR THE TREATMENT OF AUTOIMMUNE DISEASES 审中-公开
    治疗自身免疫性疾病的方法

    公开(公告)号：US20130115207A1

    公开(公告)日：2013-05-09

    申请号：US13639780

    申请日：2011-04-07

    申请人： Denise L. Faustman

    发明人： Denise L. Faustman

    IPC分类号： A61K35/76

    CPC分类号： A61K35/763 ,  A61K39/0008 ,  A61K39/04 ,  A61K2039/522 ,  A61K2039/545 ,  C12N2710/16232 ,  C12N2710/16233 ,  Y02A50/386

    摘要： The invention provides methods of treating a mammal (e.g., a human) having or at risk of having an autoimmune disease by administering a composition that includes all or a portion of a viral polypeptide or a nucleic acid encoding a viral peptide (e.g., a live, killed, attenuated, or inactivated virus) or a composition that includes an immunosuppressive agent (e.g., an anti-CD3 antibody), and compositions for use in treating an autoimmune disease in the mammal.

    摘要翻译： 本发明提供了通过施用包含病毒多肽的全部或部分或编码病毒肽的核酸（例如，活的）的组合物来治疗具有或具有自身免疫性疾病或有风险的哺乳动物（例如，人）的方法 ，灭活的，减毒的或灭活的病毒）或包含免疫抑制剂（例如抗CD3抗体）的组合物，以及用于治疗哺乳动物自身免疫疾病的组合物。

    Blood cell sorting methods and systems
    10.
    发明授权
    Blood cell sorting methods and systems 有权
    血细胞分选方法和系统

    公开(公告)号：US08187886B2

    公开(公告)日：2012-05-29

    申请号：US12987575

    申请日：2011-01-10

    申请人： Denise L. Faustman ,  Douglas E. Burger

    发明人： Denise L. Faustman ,  Douglas E. Burger

    IPC分类号： G01N33/48 ,  G01N1/18 ,  C12N13/00

    CPC分类号： C12N13/00 ,  C12N5/0087 ,  C12N5/0634 ,  G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255

    摘要： The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.

    摘要翻译： 本发明涉及使用特异性结合WBC的磁性颗粒和一系列特定步骤和条件从样品例如全血分离白细胞（WBC）的方法。 所述方法可以包括以下一种或多种：在WBC分离之前降低样品的粘度，以特定频率搅拌样品，和/或使用样品容器排列成使得所有样品都放置在非常接近的位置 ，2,1或0.5mm）到磁场源。 新方法提供了高产率，纯度和生存能力的WBC制剂的分离。 这些方法旨在与自动化协议兼容，以便快速处理多个样品。