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公开(公告)号：US08187886B2
公开(公告)日：2012-05-29
申请号：US12987575
申请日：2011-01-10
CPC分类号： C12N13/00 , C12N5/0087 , C12N5/0634 , G01N33/491 , Y10T436/10 , Y10T436/101666 , Y10T436/108331 , Y10T436/25 , Y10T436/25125 , Y10T436/25375 , Y10T436/255
摘要： The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.
摘要翻译： 本发明涉及使用特异性结合WBC的磁性颗粒和一系列特定步骤和条件从样品例如全血分离白细胞（WBC）的方法。 所述方法可以包括以下一种或多种：在WBC分离之前降低样品的粘度，以特定频率搅拌样品，和/或使用样品容器排列成使得所有样品都放置在非常接近的位置 ，2,1或0.5mm）到磁场源。 新方法提供了高产率，纯度和生存能力的WBC制剂的分离。 这些方法旨在与自动化协议兼容，以便快速处理多个样品。
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公开(公告)号：US20110177592A1
公开(公告)日：2011-07-21
申请号：US13003435
申请日：2009-07-13
CPC分类号： B03C1/288 , B03C2201/18 , B03C2201/26 , G01N33/491 , G01N33/5094 , G01N33/54326 , G01N35/0098 , G01N2446/00
摘要： An apparatus for magnetically separating constituents of blood includes: a first portion having a solid support haying fixedly attached thereon a plurality of ring magnets, each ring magnet defining an interior perimeter enclosing an aperture; and a second portion removably attached to the first portion, wherein the second portion includes a solid substrate having surfaces defining a plurality of second apertures, each second aperture configured to removably hold a vial.
摘要翻译： 用于磁性分离血液成分的装置包括：第一部分，其具有固定地附着在其上的多个环形磁体的固体支撑件，每个环形磁体限定包围孔的内部周边; 以及第二部分，其可拆卸地附接到所述第一部分，其中所述第二部分包括具有限定多个第二孔的表面的固体基底，每个第二孔被配置为可移除地保持小瓶。
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公开(公告)号：US20120295246A1
公开(公告)日：2012-11-22
申请号：US13481237
申请日：2012-05-25
IPC分类号： C12N5/078
CPC分类号： C12N13/00 , C12N5/0087 , C12N5/0634 , G01N33/491 , Y10T436/10 , Y10T436/101666 , Y10T436/108331 , Y10T436/25 , Y10T436/25125 , Y10T436/25375 , Y10T436/255
摘要： The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.
摘要翻译： 本发明涉及使用特异性结合WBC的磁性颗粒和一系列特定步骤和条件从样品例如全血分离白细胞（WBC）的方法。 所述方法可以包括以下一种或多种：在WBC分离之前降低样品的粘度，以特定频率搅拌样品，和/或使用样品容器排列成使得所有样品都放置在非常接近的位置 ，2,1或0.5mm）到磁场源。 新方法提供了高产率，纯度和生存能力的WBC制剂的分离。 这些方法旨在与自动化协议的兼容性快速处理多个样品。
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公开(公告)号：US20110111476A1
公开(公告)日：2011-05-12
申请号：US12987575
申请日：2011-01-10
CPC分类号： C12N13/00 , C12N5/0087 , C12N5/0634 , G01N33/491 , Y10T436/10 , Y10T436/101666 , Y10T436/108331 , Y10T436/25 , Y10T436/25125 , Y10T436/25375 , Y10T436/255
摘要： The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.
摘要翻译： 本发明涉及使用特异性结合WBC的磁性颗粒和一系列特定步骤和条件从样品例如全血分离白细胞（WBC）的方法。 所述方法可以包括以下一种或多种：在WBC分离之前降低样品的粘度，以特定频率搅拌样品，和/或使用样品容器排列成使得所有样品都放置在非常接近的位置 ，2,1或0.5mm）到磁场源。 新方法提供了高产率，纯度和生存能力的WBC制剂的分离。 这些方法旨在与自动化协议的兼容性快速处理多个样品。
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公开(公告)号：US08753888B2
公开(公告)日：2014-06-17
申请号：US13481237
申请日：2012-05-25
CPC分类号： C12N13/00 , C12N5/0087 , C12N5/0634 , G01N33/491 , Y10T436/10 , Y10T436/101666 , Y10T436/108331 , Y10T436/25 , Y10T436/25125 , Y10T436/25375 , Y10T436/255
摘要： The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.
摘要翻译： 本发明涉及使用特异性结合WBC的磁性颗粒和一系列特定步骤和条件从样品例如全血分离白细胞（WBC）的方法。 所述方法可以包括以下一种或多种：在WBC分离之前降低样品的粘度，以特定频率搅拌样品，和/或使用样品容器排列成使得所有样品都放置在非常接近的位置 ，2,1或0.5mm）到磁场源。 新方法提供了高产率，纯度和生存能力的WBC制剂的分离。 这些方法旨在与自动化协议的兼容性快速处理多个样品。
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公开(公告)号：US5885840A
公开(公告)日：1999-03-23
申请号：US798488
申请日：1997-02-10
CPC分类号： G01N33/52 , G01N15/1475 , G01N2015/1006
摘要： A method for increasing the accuracy and the types of data measurements of laser scanned dye stained cells, in a single sample, by means of multiple assays, utilizing cell positions as a factor in merging data measurements. Change in lasers, use of different cell dye stains and different treating reagents provide additional data regarding cells of the sample and fixing of cell positions in the first assay permits merging of the data obtained in subsequent assays.
摘要翻译： 在单个样品中，通过多次测定，利用细胞位置作为合并数据测量的因素，增加激光扫描的染色染色细胞的数据测量的精度和类型的方法。 改变激光，使用不同的细胞染料污渍和不同的处理试剂提供关于样品细胞的另外的数据，并且在第一次测定中固定细胞位置允许合并后续测定中获得的数据。
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公开(公告)号：US07867765B2
公开(公告)日：2011-01-11
申请号：US11646985
申请日：2006-12-28
CPC分类号： C12N13/00 , C12N5/0087 , C12N5/0634 , G01N33/491 , Y10T436/10 , Y10T436/101666 , Y10T436/108331 , Y10T436/25 , Y10T436/25125 , Y10T436/25375 , Y10T436/255
摘要： The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.
摘要翻译： 本发明涉及使用特异性结合WBC的磁性颗粒和一系列特定步骤和条件从样品例如全血分离白细胞（WBC）的方法。 所述方法可以包括以下一种或多种：在WBC分离之前降低样品的粘度，以特定频率搅拌样品，和/或使用样品容器排列成使得所有样品都放置在非常接近的位置 ，2,1或0.5mm）到磁场源。 新方法提供了高产率，纯度和生存能力的WBC制剂的分离。 这些方法旨在与自动化协议兼容，以便快速处理多个样品。
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