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    Blood cell sorting methods and systems
    1.
    发明授权
    Blood cell sorting methods and systems 有权
    血细胞分选方法和系统

    公开(公告)号：US07867765B2

    公开(公告)日：2011-01-11

    申请号：US11646985

    申请日：2006-12-28

    申请人： Denise L. Faustman ,  Douglas E. Burger

    发明人： Denise L. Faustman ,  Douglas E. Burger

    IPC分类号： G01N33/48 ,  G01N1/18

    CPC分类号： C12N13/00 ,  C12N5/0087 ,  C12N5/0634 ,  G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255

    摘要： The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.

    摘要翻译： 本发明涉及使用特异性结合WBC的磁性颗粒和一系列特定步骤和条件从样品例如全血分离白细胞（WBC）的方法。 所述方法可以包括以下一种或多种：在WBC分离之前降低样品的粘度，以特定频率搅拌样品，和/或使用样品容器排列成使得所有样品都放置在非常接近的位置 ，2,1或0.5mm）到磁场源。 新方法提供了高产率，纯度和生存能力的WBC制剂的分离。 这些方法旨在与自动化协议兼容，以便快速处理多个样品。

    Blood cell sorting methods and systems
    2.
    发明授权
    Blood cell sorting methods and systems 有权
    血细胞分选方法和系统

    公开(公告)号：US08753888B2

    公开(公告)日：2014-06-17

    申请号：US13481237

    申请日：2012-05-25

    申请人： Denise L. Faustman ,  Douglas E. Burger

    发明人： Denise L. Faustman ,  Douglas E. Burger

    IPC分类号： G01N33/48 ,  G01N1/18 ,  C12N13/00

    CPC分类号： C12N13/00 ,  C12N5/0087 ,  C12N5/0634 ,  G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255

    摘要： The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.

    摘要翻译： 本发明涉及使用特异性结合WBC的磁性颗粒和一系列特定步骤和条件从样品例如全血分离白细胞（WBC）的方法。 所述方法可以包括以下一种或多种：在WBC分离之前降低样品的粘度，以特定频率搅拌样品，和/或使用样品容器排列成使得所有样品都放置在非常接近的位置 ，2,1或0.5mm）到磁场源。 新方法提供了高产率，纯度和生存能力的WBC制剂的分离。 这些方法旨在与自动化协议的兼容性快速处理多个样品。

    Blood cell sorting methods and systems
    3.
    发明授权
    Blood cell sorting methods and systems 有权
    血细胞分选方法和系统

    公开(公告)号：US08187886B2

    公开(公告)日：2012-05-29

    申请号：US12987575

    申请日：2011-01-10

    申请人： Denise L. Faustman ,  Douglas E. Burger

    发明人： Denise L. Faustman ,  Douglas E. Burger

    IPC分类号： G01N33/48 ,  G01N1/18 ,  C12N13/00

    CPC分类号： C12N13/00 ,  C12N5/0087 ,  C12N5/0634 ,  G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255

    摘要： The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.

    摘要翻译： 本发明涉及使用特异性结合WBC的磁性颗粒和一系列特定步骤和条件从样品例如全血分离白细胞（WBC）的方法。 所述方法可以包括以下一种或多种：在WBC分离之前降低样品的粘度，以特定频率搅拌样品，和/或使用样品容器排列成使得所有样品都放置在非常接近的位置 ，2,1或0.5mm）到磁场源。 新方法提供了高产率，纯度和生存能力的WBC制剂的分离。 这些方法旨在与自动化协议兼容，以便快速处理多个样品。

    MAGNETIC APPARATUS FOR BLOOD SEPARATION
    4.
    发明申请
    MAGNETIC APPARATUS FOR BLOOD SEPARATION 审中-公开
    用于血液分离的磁性装置

    公开(公告)号：US20110177592A1

    公开(公告)日：2011-07-21

    申请号：US13003435

    申请日：2009-07-13

    申请人： Denise L. Faustman ,  Douglas E. Burger

    发明人： Denise L. Faustman ,  Douglas E. Burger

    IPC分类号： C12N5/02 ,  C12M1/00

    CPC分类号： B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  G01N33/491 ,  G01N33/5094 ,  G01N33/54326 ,  G01N35/0098 ,  G01N2446/00

    摘要： An apparatus for magnetically separating constituents of blood includes: a first portion having a solid support haying fixedly attached thereon a plurality of ring magnets, each ring magnet defining an interior perimeter enclosing an aperture; and a second portion removably attached to the first portion, wherein the second portion includes a solid substrate having surfaces defining a plurality of second apertures, each second aperture configured to removably hold a vial.

    摘要翻译： 用于磁性分离血液成分的装置包括：第一部分，其具有固定地附着在其上的多个环形磁体的固体支撑件，每个环形磁体限定包围孔的内部周边; 以及第二部分，其可拆卸地附接到所述第一部分，其中所述第二部分包括具有限定多个第二孔的表面的固体基底，每个第二孔被配置为可移除地保持小瓶。

    BLOOD CELL SORTING METHODS AND SYSTEMS
    5.
    发明申请
    BLOOD CELL SORTING METHODS AND SYSTEMS 有权
    血细胞分选方法和系统

    公开(公告)号：US20120295246A1

    公开(公告)日：2012-11-22

    申请号：US13481237

    申请日：2012-05-25

    申请人： Denise L. Faustman ,  Douglas E. Burger

    发明人： Denise L. Faustman ,  Douglas E. Burger

    IPC分类号： C12N5/078

    CPC分类号： C12N13/00 ,  C12N5/0087 ,  C12N5/0634 ,  G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255

    摘要： The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.

    摘要翻译： 本发明涉及使用特异性结合WBC的磁性颗粒和一系列特定步骤和条件从样品例如全血分离白细胞（WBC）的方法。 所述方法可以包括以下一种或多种：在WBC分离之前降低样品的粘度，以特定频率搅拌样品，和/或使用样品容器排列成使得所有样品都放置在非常接近的位置 ，2,1或0.5mm）到磁场源。 新方法提供了高产率，纯度和生存能力的WBC制剂的分离。 这些方法旨在与自动化协议的兼容性快速处理多个样品。

    BLOOD CELL SORTING METHODS AND SYSTEMS
    6.
    发明申请
    BLOOD CELL SORTING METHODS AND SYSTEMS 有权
    血细胞分选方法和系统

    公开(公告)号：US20110111476A1

    公开(公告)日：2011-05-12

    申请号：US12987575

    申请日：2011-01-10

    申请人： Denise L. Faustman ,  Douglas E. Burger

    发明人： Denise L. Faustman ,  Douglas E. Burger

    IPC分类号： C12N13/00 ,  C12N5/078

    CPC分类号： C12N13/00 ,  C12N5/0087 ,  C12N5/0634 ,  G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255

    摘要： The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.

    摘要翻译： 本发明涉及使用特异性结合WBC的磁性颗粒和一系列特定步骤和条件从样品例如全血分离白细胞（WBC）的方法。 所述方法可以包括以下一种或多种：在WBC分离之前降低样品的粘度，以特定频率搅拌样品，和/或使用样品容器排列成使得所有样品都放置在非常接近的位置 ，2,1或0.5mm）到磁场源。 新方法提供了高产率，纯度和生存能力的WBC制剂的分离。 这些方法旨在与自动化协议的兼容性快速处理多个样品。

    Stem cells and their use in transplantation
    8.
    发明授权
    Stem cells and their use in transplantation 有权
    干细胞及其在移植中的应用

    公开(公告)号：US07537756B2

    公开(公告)日：2009-05-26

    申请号：US11132687

    申请日：2005-05-19

    申请人： Joel E. Habener ,  Hendrik Zulewski ,  Elizabeth J. Abraham ,  Mario Vallejo ,  Denise L. Faustman ,  Melissa K. Thomas

    发明人： Joel E. Habener ,  Hendrik Zulewski ,  Elizabeth J. Abraham ,  Mario Vallejo ,  Denise L. Faustman ,  Melissa K. Thomas

    IPC分类号： A61K35/12 ,  C12N5/00

    CPC分类号： C12N5/0676 ,  A01K67/0271 ,  A61K35/12 ,  A61K2035/122 ,  C12N5/0677 ,  C12N5/0678 ,  C12N2500/34 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/335 ,  C12N2510/02

    摘要： Methods and compositions are described for the treatment of type I insulin-dependent diabetes mellitus and other conditions using newly identified stem cells that are capable of differentiation into a variety of pancreatic islet cells, including insulin-producing beta cells, as well as hepatocytes. Nestin has been identified as a molecular marker for pancreatic stem cells, while cytokeratin-19 serves as a marker for a distinct class of islet ductal cells. Methods are described whereby nestin-positive stem cells can be isolated from pancreatic islets and cultured to obtain further stem cells or pseudo-islet like structures. Methods for ex vivo differentiation of the pancreatic stem cells are disclosed. Methods are described whereby pancreatic stem cells can be isolated, expanded, and transplanted into a patient in need thereof, either allogeneically, isogeneically or xenogenically, to provide replacement for lost or damaged insulin-secreting cells or other cells.

    摘要翻译： 描述了用于治疗I型胰岛素依赖性糖尿病和其它条件的方法和组合物，其使用能够分化成多种胰岛细胞（包括产生胰岛素的β细胞）以及肝细胞的新鉴定的干细胞。 巢蛋白已被鉴定为胰腺干细胞的分子标记，而细胞角蛋白-19可作为不同类别的胰岛导管细胞的标记物。 描述了方法，其中可以从胰岛中分离巢蛋白阳性干细胞并培养以获得进一步的干细胞或假胰岛样结构。 公开了胰腺干细胞离体分化方法。 描述了方法，其中胰腺干细胞可以被分离，扩增和移植到有需要的患者中，同种异体地，异质性或异质性的，以提供丢失或损伤的胰岛素分泌细胞或其他细胞的替代。