Use of anthocyanin genes to maintain male sterile plants
    3.
    发明授权
    Use of anthocyanin genes to maintain male sterile plants 失效
    使用花青素基因维持雄性不育植物

    公开(公告)号:US6008437A

    公开(公告)日:1999-12-28

    申请号:US750357

    申请日:1997-03-21

    CPC分类号: C12N15/8289

    摘要: A plant consisting essentially of cells which comprise in their genome a homozygous male-sterility genotype at a first genetic locus; and a color-linked restorer genotype at a second genetic locus, which is heterozygous (Rf/-) for a foreign DNA Rf. The foreign DNA Rf comprises: a) a fertility-restorer gene capable of preventing the phenotypic expression of the male-sterility genotype, and b) at least one anthocyanin regulatory gene involved in the regulation of anthocyanin biosynthesis in cells of seeds of the plant which is capable of producing anthocyanin at least in the seeds of the plant, so that anthocyanin production in the seeds is visible externally. Preferably, the anthocyanin regulatory gene is a shortened R, B or C1 gene or a continuation thereof. The invention also relates to DNA sequences encoding shortened R, B or C1 anthocyanin regulatory genes and to a process for maintaining a line of male-sterile plants which comprises crossing a male-sterile parent plant and a maintainer parent plant comprising homozygous male-sterility genotype and a restore genotype comprising fertility-restorer gene and an anthocyanin regulatory gene.

    摘要翻译: PCT No.PCT / EP95 / 02157 Sec。 371日期1997年3月21日 102(e)日期1997年3月21日PCT归档1995年6月6日PCT公布。 WO95 / 34634 PCT出版物 日期1995年12月21日一种基本上由细胞组成的植物,其在基因组中在第一遗传基因座处包含纯合的雄性不育基因型; 和在第二个遗传基因座上的染色连接的恢复基因型,其对于外源DNA Rf是杂合的(Rf / - )。 外源DNA Rf包括:a)能够预防雄性不育基因型表型表达的育性恢复基因,以及b)至少一种参与植物种子细胞中花青素生物合成调节的花青素调节基因, 能够至少在植物的种子中产生花青素,使得种子中的花青素生产在外部是可见的。 优选地,花青素调节基因是缩短的R,B或C1基因或其延续。 本发明还涉及编码缩短的R,B或C1花青素调节基因的DNA序列,以及保持一系列雄性不育植物的方法,其包括将雄性不育亲本植物和包含纯合雄性不育基因型的维持母体植物杂交 以及包含生育恢复基因和花青素调节基因的恢复基因型。

    Use of anthocyanin genes to maintain male sterile plants
    4.
    发明授权
    Use of anthocyanin genes to maintain male sterile plants 失效
    使用花青素基因维持雄性不育植物

    公开(公告)号:US5880331A

    公开(公告)日:1999-03-09

    申请号:US485139

    申请日:1995-06-07

    摘要: This invention is directed to a method to maintain male sterility in corn by the genetic linkage via genetic engineering of a homozygous male-fertility gene with a color linked restorer and plant comprising said genes. Color genes disclosed as useful in this invention include those involved in anthocyanin biosynthesis, which in corn are under the control of as many as 20 or more genes. In particular, the use of the anthocyanin color genes R, B and C1, and specific regulatory elements sequences are taught.

    摘要翻译: 本发明涉及一种通过遗传连锁通过遗传连锁维持雄性不育性的方法,所述遗传连锁通过纯合雄性 - 生育基因与含有所述基因的色素还原剂和植物的遗传工程。 公开的用于本发明的彩色基因包括参与花青素生物合成的那些,其在玉米中可以控制多达20个或更多个基因。 特别地,教导了使用花青素颜色基因R,B和C1以及特定的调节元件序列。

    Plant viral movement protein genes
    6.
    发明授权
    Plant viral movement protein genes 有权
    植物病毒运动蛋白基因

    公开(公告)号:US07572951B2

    公开(公告)日:2009-08-11

    申请号:US11653567

    申请日:2007-01-16

    摘要: This invention relates to an isolated nucleic acid fragment encoding a viral movement protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the viral movement protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the viral movement protein in a transformed host cell.

    摘要翻译: 本发明涉及编码病毒运动蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码病毒运动蛋白的全部或大部分的嵌合基因,其中嵌合基因的表达导致转化宿主中病毒运动蛋白水平的改变 细胞。

    Plant viral movement protein genes
    7.
    发明申请
    Plant viral movement protein genes 有权
    植物病毒运动蛋白基因

    公开(公告)号:US20070186309A1

    公开(公告)日:2007-08-09

    申请号:US11653567

    申请日:2007-01-16

    摘要: This invention relates to an isolated nucleic acid fragment encoding a viral movement protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the viral movement protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the viral movement protein in a transformed host cell.

    摘要翻译: 本发明涉及编码病毒运动蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码病毒运动蛋白的全部或大部分的嵌合基因,其中嵌合基因的表达导致转化宿主中病毒运动蛋白水平的改变 细胞。

    Methods and compositions for gene silencing
    9.
    发明申请
    Methods and compositions for gene silencing 审中-公开
    用于基因沉默的方法和组合物

    公开(公告)号:US20070130653A1

    公开(公告)日:2007-06-07

    申请号:US11455073

    申请日:2006-06-16

    摘要: Methods and compositions are provided for reducing the level of expression of a target polynucleotide in an organism. The methods and compositions selectively silence the target polynucleotide through the expression of a chimeric polynucleotide comprising the target for a sRNA (the trigger sequence) operably linked to a sequence corresponding to all or part of the gene or genes to be silenced. In this manner, the final target of silencing is an endogenous gene in the organism in which the chimeric polynucleotide is expressed. In a further embodiment, the miRNA target is that of a heterologous miRNA or siRNA, the latter of which is coexpressed in the cells at the appropriate developmental stage to provide silencing of the final target when and where desired. In a further embodiment, the final target may be a gene in a second organism, such as a plant pest, that feeds upon the organism containing the chimeric gene or genes. Compositions further comprise vectors, seeds, grain, cells, and organisms, including plants and plant cells, comprising the chimeric polynucleotide of the invention.

    摘要翻译: 提供了用于降低生物体中靶多核苷酸表达水平的方法和组合物。 所述方法和组合物通过表达嵌合多核苷酸来选择性沉默靶多核苷酸,所述嵌合多核苷酸包含可操作地连接到对应于待沉默的基因或基因的全部或部分的序列的sRNA(触发序列)的靶。 以这种方式,沉默的最终靶标是其中表达嵌合多核苷酸的生物体中的内源基因。 在另一个实施方案中,miRNA靶是异源miRNA或siRNA的靶标,其后者在合适的发育阶段在细胞中共表达,以在需要时和期望时提供最终靶的沉默。 在另一个实施方案中,最终靶可以是在含有嵌合基因或基因的生物体上进食的第二生物体如植物害虫中的基因。 组合物还包含载体,种子,谷粒,细胞和生物,包括植物和植物细胞,其包含本发明的嵌合多核苷酸。

    Corn 4-.alpha.-glucanotransferase
    10.
    发明授权
    Corn 4-.alpha.-glucanotransferase 失效
    玉米4-α-葡聚糖转移酶

    公开(公告)号:US5994623A

    公开(公告)日:1999-11-30

    申请号:US838543

    申请日:1997-04-09

    CPC分类号: C12N9/1051 C12N15/8245

    摘要: This invention relates to isolated nucleic acid fragments encoding all or a substantial portion of a corn 4-.alpha.-glucanotransferase. The invention also relates to the construction of chimeric genes encoding all or a portion of a corn 4-.alpha.-glucanotransferase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of corn 4-.alpha.-glucanotransferase in a transformed host cell.

    摘要翻译: 本发明涉及编码玉米4-α-葡聚糖转移酶的全部或大部分的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码玉米4-α-葡聚糖转移酶的全部或部分的嵌合基因,其中嵌合基因的表达导致产生改变水平的玉米4-α-葡聚糖转移酶 转化的宿主细胞。