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公开(公告)号：US20130251726A1
公开(公告)日：2013-09-26
申请号：US13792098
申请日：2013-03-10
申请人： John Mascola , Dennis R. Burton , Wayne Koff , Peter Kwong , Gary Nabel , Sanjay K. Phogat , Pascal Raymond Georges Poignard , Melissa Danielle De Jean St. Marcel Simek-Lemos , Xueling Wu , Tongqing Zhou , Zhi-Yong Yang
发明人： John Mascola , Dennis R. Burton , Wayne Koff , Peter Kwong , Gary Nabel , Sanjay K. Phogat , Pascal Raymond Georges Poignard , Melissa Danielle De Jean St. Marcel Simek-Lemos , Xueling Wu , Tongqing Zhou , Zhi-Yong Yang
CPC分类号： C07K16/1063 , A61K39/42 , A61K45/06 , A61K2039/505 , C07K2317/21 , C07K2317/33 , C07K2317/34 , C07K2317/76 , C07K2317/92 , G01N33/56988
摘要： The present application relates novel HIV-1 broadly neutralizing antibodies. The antibodies of the present invention are further characterized by their ability to bind epitopes from the Env proteins. The invention also provides light and heavy chain variable region sequences. Compositions for prophylaxis, diagnosis and treatment of HIV infection are provided.
摘要翻译： 本申请涉及新型HIV-1广泛中和抗体。 本发明的抗体的特征还在于它们能够结合来自Env蛋白的表位。 本发明还提供轻链和重链可变区序列。 提供了预防，诊断和治疗艾滋病毒感染的组合。
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2.发明授权Monoclonal antibodies directed against trimeric forms of the HIV-1 envelope glycoprotein with broad and potent neutralizing activity 有权针对三聚体形式的HIV-1包膜糖蛋白的单克隆抗体具有广泛和有效的中和活性公开(公告)号：US09051362B2
公开(公告)日：2015-06-09
申请号：US12726245
申请日：2010-03-17
申请人： Po-Ying Chan-Hui , Steven Frey , Ole Olsen , Jennifer Mitcham , Matthew Moyle , Sanjay K. Phogat , Dennis R. Burton , Laura Marjorie Walker , Pascal Raymond Georges Poignard , Wayne Koff , Melissa Danielle De Jean De St. Marcel Simek-Lemos
发明人： Po-Ying Chan-Hui , Steven Frey , Ole Olsen , Jennifer Mitcham , Matthew Moyle , Sanjay K. Phogat , Dennis R. Burton , Laura Marjorie Walker , Pascal Raymond Georges Poignard , Wayne Koff , Melissa Danielle De Jean De St. Marcel Simek-Lemos
CPC分类号： C07K16/1045 , A61K39/21 , A61K2039/505 , C07K16/1063 , C07K2317/56 , C07K2317/565 , C07K2317/76
摘要： The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies are characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.
摘要翻译： 本发明提供一种获得广泛中和抗体（bNab）的方法，包括筛选来自供体PBMC样品的记忆B细胞培养物，以对多种HIV-1物质进行中和活性，克隆显示广泛中和活性的记忆B细胞; 并从该记忆B细胞培养物中拯救单克隆抗体。 所得单克隆抗体的特征在于它们以天然或单体形式选择性地结合来自Env蛋白的表位的能力，以及抑制HIV-1物种从多个进化枝的感染。 提供了含有用于预防，诊断和治疗HIV感染的人单克隆抗HIV抗体的组合物。 提供了通过使用来自gp120的可变环内的保守区域的表位进行免疫来产生这种抗体的方法。 还提供了产生抗艾滋病毒1型免疫球蛋白的免疫原。 此外，提供了使用合适表位的疫苗接种方法。
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公开(公告)号：US20140205612A1
公开(公告)日：2014-07-24
申请号：US13780776
申请日：2013-02-28
申请人： Po-Ying Chan-Hui , Katherine Doores , Michael Huber , Stephen Kaminsky , Steven Frey , Ole Olsen , Jennifer Mitcham , Matthew Moyle , Sanjay K. Phogat , Dennis R. Burton , Laura Marjorie Walker , Pascal Raymond Georges Poignard , Wayne Koff , Melissa Danielle De Jean De St. Marcel Simek-Lemos
发明人： Po-Ying Chan-Hui , Katherine Doores , Michael Huber , Stephen Kaminsky , Steven Frey , Ole Olsen , Jennifer Mitcham , Matthew Moyle , Sanjay K. Phogat , Dennis R. Burton , Laura Marjorie Walker , Pascal Raymond Georges Poignard , Wayne Koff , Melissa Danielle De Jean De St. Marcel Simek-Lemos
CPC分类号： C07K16/1063 , A61K39/21 , A61K2039/505 , C07K16/1045 , C07K2317/21 , C07K2317/33 , C07K2317/34 , C07K2317/51 , C07K2317/515 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2317/92
摘要： The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.
摘要翻译： 本发明提供了一种获得广泛中和抗体（bNab）的方法，包括筛选来自供体PBMC样品的记忆B细胞培养物，以抵抗多种HIV-1物种的中和活性，克隆显示广泛中和活性的记忆B细胞; 并从该记忆B细胞培养物中拯救单克隆抗体。 所得到的单克隆抗体的特征在于它们以天然或单体形式选择性地结合来自Env蛋白的表位的能力，以及抑制HIV-1物种从多个进化枝的感染的能力。 提供了含有用于预防，诊断和治疗HIV感染的人单克隆抗HIV抗体的组合物。 提供了通过使用来自gp120的可变环内的保守区域的表位进行免疫来产生这种抗体的方法。 还提供了产生抗艾滋病毒1型免疫球蛋白的免疫原。 此外，提供了使用合适表位的疫苗接种方法。
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公开(公告)号：US20110044994A1
公开(公告)日：2011-02-24
申请号：US12726245
申请日：2010-03-17
申请人： Po-Ying Chan-Hui , Steven Frey , Ole Olsen , Jennifer Mitcham , Matthew Moyle , Sanjay K. Phogat , Dennis R. Burton , Laura Marjorie Walker , Pascal Raymond Georges Poignard , Wayne Koff , Melissa Danielle De Jean De St. Marcel Simek-Lemos
发明人： Po-Ying Chan-Hui , Steven Frey , Ole Olsen , Jennifer Mitcham , Matthew Moyle , Sanjay K. Phogat , Dennis R. Burton , Laura Marjorie Walker , Pascal Raymond Georges Poignard , Wayne Koff , Melissa Danielle De Jean De St. Marcel Simek-Lemos
IPC分类号： A61K39/42 , C07K16/10 , C07K16/00 , C07H21/04 , C12N15/63 , C12N5/10 , A61K39/395 , A61K39/21 , A61P31/18
CPC分类号： C07K16/1045 , A61K39/21 , A61K2039/505 , C07K16/1063 , C07K2317/56 , C07K2317/565 , C07K2317/76
摘要： The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies are characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.
摘要翻译： 本发明提供了一种获得广泛中和抗体（bNab）的方法，包括筛选来自供体PBMC样品的记忆B细胞培养物，以抵抗多种HIV-1物质的中和活性，克隆显示广泛中和活性的记忆B细胞; 并从该记忆B细胞培养物中拯救单克隆抗体。 所得单克隆抗体的特征在于它们以天然或单体形式选择性地结合来自Env蛋白的表位的能力，以及抑制HIV-1物种从多个进化枝的感染。 提供了含有用于预防，诊断和治疗HIV感染的人单克隆抗HIV抗体的组合物。 提供了通过使用来自gp120的可变环内的保守区域的表位进行免疫来产生这种抗体的方法。 还提供了产生抗艾滋病毒1型免疫球蛋白的免疫原。 此外，提供了使用合适表位的疫苗接种方法。
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公开(公告)号：US06531313B1
公开(公告)日：2003-03-11
申请号：US09697236
申请日：2000-10-26
申请人： Jaap Goudsmit , Jerald C. Sadoff , Wayne Koff
发明人： Jaap Goudsmit , Jerald C. Sadoff , Wayne Koff
IPC分类号： C12N1585
CPC分类号： C12N15/63 , A61K39/00 , A61K39/12 , A61K48/00 , A61K2039/523 , A61K2039/5256 , C12N15/85 , C12N15/86 , C12N15/87 , C12N2740/16034 , C12N2760/14034 , C12N2770/36143 , Y02A50/476 , Y02A50/484
摘要： The present invention is directed to a bacterial delivery system for delivering alphavirus replicon DNA into an animal or animal cells with the replicon encoding one or more heterologous genes to be expressed in the animal or the animal cells. The bacteria are invasive bacteria or attenuated invasive bacteria engineered to contain a DNA vector that encodes the alphavirus replicon in a eukaryotic expression cassette. The heterologous gene can encode an antigen, a therapeutic agent, an immunoregulatory agent, an anti-sense RNA, a catalytic RNA, a protein, a peptide, an antibody or an antigen-binding fragment of an antibody. In a preferred embodiment, the heterologous gene encodes one or more antigens useful as a vaccine for HIV. In addition to the bacterial delivery system, the invention provides methods of introducing and expressing the heterologous gene(s) in animal or animal cells and methods of stimulating or inducing an immune response.
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