公开(公告)号：US06528622B1

公开(公告)日：2003-03-04

申请号：US09117401

申请日：1999-04-30

申请人： John Stephen Ayers ,  David Francis Elgar ,  Mark Pritchard

发明人： John Stephen Ayers ,  David Francis Elgar ,  Mark Pritchard

IPC分类号： C07K118

CPC分类号： A23J3/08 ,  A23C9/1465 ,  A23J1/205 ,  Y10S530/833

摘要： A preparative method of isolating a preselected whey protein or group of whey proteins from a solution is provided. The method comprises the following steps: (a) contacting a whey protein solution with the preselected ion exchanger for a time and at a temperature sufficient to enable the preselected whey protein to be adsorbed; wherein the whey protein solution has (1) a protein content in the range of about 5% to about 20% by weight, (2) a pH of a preselected level, which is the level at which the preselected whey protein or group of whey proteins selectively binds to the preselected ion exchanger, and (3) a reduced ionic strength; and (b) recovering either or both of the following: (1) the whey protein component adsorbed in step (a), and (2) the breakthrough whey protein component not adsorbed in step (a). It is preferred that the whey protein solution is a retentate obtained via ultrafiltration of whey, having reduced ionic strength, or a whey protein concentrate powder which has been reconstituted with water.

摘要翻译： 提供了从溶液中分离预选乳清蛋白或一组乳清蛋白的制备方法。 该方法包括以下步骤：（a）将乳清蛋白溶液与预选离子交换剂接触一段时间并且足以使预选乳清蛋白被吸附的温度; 其中所述乳清蛋白溶液具有（1）蛋白质含量在约5％至约20％重量的范围内，（2）预选水平的pH，其是预选乳清蛋白或乳清组的水平 蛋白质选择性地结合预选离子交换剂，和（3）降低的离子强度; 和（b）回收下列各项之一或两者：（1）步骤（a）中吸附的乳清蛋白组分，和（2）步骤（a）中未吸附的穿透乳清蛋白组分。 优选乳清蛋白溶液是通过具有降低的离子强度的乳清超滤获得的保留物，或已经用水重构的乳清蛋白浓缩物粉末。

公开(公告)号：US07018665B2

公开(公告)日：2006-03-28

申请号：US10149339

申请日：2000-12-08

申请人： John Stephen Ayers ,  David Francis Elgar ,  Kay Patricia Palmano ,  Mark Pritchard ,  Ganugapati Bijaya Bhaskar

发明人： John Stephen Ayers ,  David Francis Elgar ,  Kay Patricia Palmano ,  Mark Pritchard ,  Ganugapati Bijaya Bhaskar

IPC分类号： A23C21/00

CPC分类号： B01J41/16 ,  A23C9/1465 ,  A23J1/205 ,  A23V2002/00 ,  A23V2250/54242 ,  A23V2250/5434 ,  A23V2300/30

摘要： The present invention relates to new processes useful for separating whey proteins from whey protein-containing solutions using a novel anion exchanger which comprises a water insoluble, hydrophilic, water swellable, hydroxy (C2–C4) alkylated and cross-linked regenerated cellulose, derivatized with quaternary amino (QA) groups where the level of substitution of the QA groups is 1.4 milli-equivalents per dry gram of anion exchanger (meq/g) or greater.

摘要翻译： 本发明涉及用于从含乳清蛋白的溶液中分离乳清蛋白的新方法，该新型阴离子交换剂包含水不溶性，亲水性，水可溶胀的羟基（C 2 -C 2 - 4QA）烷基化和交联的再生纤维素，其季铵基（QA）基团衍生化，其中QA基团的取代水平为1.4毫当量/干燥阴离子交换剂（meq / g）或更大。

公开(公告)号：US06592905B1

公开(公告)日：2003-07-15

申请号：US09117631

申请日：1998-11-16

申请人： John Stephen Ayers ,  David Francis Elgar ,  Mark Pritchard

发明人： John Stephen Ayers ,  David Francis Elgar ,  Mark Pritchard

IPC分类号： A61K3520

CPC分类号： A23J1/205 ,  A23C9/1465

摘要： This invention is directed to a process for producing an immunoglobulin enriched whey protein fraction from whey protein solutions. More particularly, it relates to the production of a whey protein fraction enriched in immunoglobulin, and optionally a whey protein isolate, using a cation exchanger under selected conditions. The selected conditions require overloading the cation exchanger with potentially absorbable protein which causes the exchanger to adsorb preferentially whey proteins other than immunoglobulin. The invention is also directed to the products produced by the process of the invention.

摘要翻译： 本发明涉及一种从乳清蛋白溶液中生产免疫球蛋白的乳清蛋白级分的方法。 更具体地说，本发明涉及使用阳离子交换剂在选择的条件下生产富含免疫球蛋白的乳清蛋白质级分和任选的乳清蛋白分离物。 所选择的条件需要使潜在的可吸收蛋白质使阳离子交换剂过载，这导致交换剂优先吸附除免疫球蛋白以外的乳清蛋白。 本发明还涉及通过本发明的方法生产的产品。

公开(公告)号：US06555659B1

公开(公告)日：2003-04-29

申请号：US09625043

申请日：2000-07-24

申请人： John Stephen Ayers ,  Kay Patricia Coolbear ,  David Francis Elgar ,  Mark Pritchard

发明人： John Stephen Ayers ,  Kay Patricia Coolbear ,  David Francis Elgar ,  Mark Pritchard

IPC分类号： C07K1400

CPC分类号： A23J1/202 ,  C07K14/4732 ,  Y10S530/832 ,  Y10S530/833

摘要： The present invention is related to a method for the purification of glycomacropeptide (GMP) with an amino acid composition containing no greater that 0.5% (w/w) phenylalanine, comprising the steps of contacting a GMP-containing feedstock with a first anion exchanger under conditions to adsorb the GMP, eluting the adsorbed GMP from the anion exchanger and removing impurities from the GMP-containing eluate by either: (i) contacting the GMP-containing eluate with a cation exchanger in conditions under which the impurities in the eluate are adsorbed onto the cation exchanger, or (ii) precipitating the impurities in GMP-containing eluate using conditions in which the GMP remains i solution, or (iii) contacting the GMP-containing eluate with a second anion exchanger in conditions under which the impurities in the eluate are adsorbed onto the anion exchanger, and recovering the GMP from whichever one or more of the steps (i), (ii) or (iii) was used.

摘要翻译： 本发明涉及一种纯化含有不大于0.5％（w / w）苯丙氨酸的氨基酸组成的糖巨肽（GMP）的方法，该方法包括以下步骤：使含GMP的原料与第一阴离子交换剂接触 吸附GMP的条件，从阴离子交换器洗脱吸附的GMP并从含GMP的洗脱液中除去杂质，方法是：（i）在吸附洗脱液中的杂质的条件下将含GMP的洗脱液与阳离子交换剂接触 或者（ii）使用GMP保留在溶液中的条件沉淀含GMP的洗脱液中的杂质，或者（iii）使含GMP的洗脱液与第二阴离子交换剂接触， 洗脱液被吸附到阴离子交换器上，并且从使用步骤（i），（ii）或（iii）中的一个或多个步骤回收GMP。

公开(公告)号：US06911483B2

公开(公告)日：2005-06-28

申请号：US10149332

申请日：2000-12-08

申请人： John Stephen Ayers ,  David Francis Elgar ,  Margaret Mary Agnes Egan

发明人： John Stephen Ayers ,  David Francis Elgar ,  Margaret Mary Agnes Egan

IPC分类号： A23C9/146 ,  A23J1/20 ,  B01J41/16 ,  C08J5/20

CPC分类号： B01J41/16 ,  A23C9/1465 ,  A23J1/205 ,  A23V2002/00 ,  A23V2250/54242 ,  A23V2250/5434 ,  A23V2300/30

摘要： The present invention is related to a new quaternary amino (QA) anion exchanger comprising QA derivatized, hydroxy (C2-C4) alkylated and cross-linked regenerated cellulose, in which the level of derivitization with the QA-groups is 1.4 milli-equivalents per dry gram (meq/g) or greater.

摘要翻译： 本发明涉及一种新的季铵（QA）阴离子交换剂，其包含QA衍生的羟基（C 2 -C 4 -C 4 - 烷基）和交联的再生纤维素，其中 其中QA基团的衍生水平为1.4毫当量/干克（meq / g）或更高。

公开(公告)号：US4096136A

公开(公告)日：1978-06-20

申请号：US773270

申请日：1977-03-01

申请人： John Stephen Ayers ,  David Roderick Husbands

发明人： John Stephen Ayers ,  David Roderick Husbands

IPC分类号： G01N33/68 ,  A61K35/14 ,  A61K35/16 ,  B01J20/281 ,  B01J39/22 ,  B01J47/00 ,  G01N30/00 ,  G01N30/88 ,  G01N33/49 ,  G01N33/92 ,  C07G7/00

CPC分类号： B01J39/22 ,  A61K35/16 ,  B01J47/003 ,  G01N30/00 ,  G01N33/491 ,  G01N33/92 ,  Y10S530/83 ,  Y10T436/25125

摘要： The invention relates to the use of a sulphated ion exchanger to selectively remove lipoproteins from blood plasma or serum. Although the attraction between sulphate groups and proteins has long been recognized it has now unexpectedly been found that cationic ion exchangers whose ion exchange capacity is provided by sulphate groups bind lipoproteins selectively in presence of other plasma or serum proteins and ions when they are equilibrated with solutions containing divalent cations and the same cation is added to the plasma or serum. Types of ion exchanger matrices and applications of the utlity are discussed.

摘要翻译： 本发明涉及使用硫酸化离子交换剂来选择性地从血浆或血清中除去脂蛋白。 虽然长期以来已经认识到硫酸盐基团和蛋白质之间的吸引力已经意外地发现，当离子交换容量由硫酸盐基团提供的阳离子交换剂在其它血浆或血清蛋白质和离子的存在下在选择性地结合脂蛋白时使用溶液平衡 含有二价阳离子并将相同的阳离子加入到血浆或血清中。 讨论了离子交换矩阵的类型及其应用。