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    Homogeneous populations of molecules
    4.
    发明申请
    Homogeneous populations of molecules 有权
    均质群体的分子

    公开(公告)号：US20050106605A1

    公开(公告)日：2005-05-19

    申请号：US10949471

    申请日：2004-09-24

    申请人： Joseph Amshey ,  Roumen Bogoev

    发明人： Joseph Amshey ,  Roumen Bogoev

    IPC分类号： C07H21/04 ,  C12N9/64 ,  C12Q20060101 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/68

    CPC分类号： G01N33/6842 ,  C40B40/10 ,  G01N2496/00

    摘要： The invention provides populations of molecules that are prepared as, or treated to become, homogeneous for one or more molecular characteristics. In an aspect, the invention relates to molecular weight standards that may be used to determine the molecular weight or apparent molecular weight of uncharacterized molecules, such as proteins and nucleic acids, as well as in other applications. In one aspect, the molecular weight standards are pre-stained.

    摘要翻译： 本发明提供了为一种或多种分子特征制备或被处理成均匀的分子群体。 在一方面，本发明涉及可用于测定未表征的分子（例如蛋白质和核酸）以及其它应用的分子量或表观分子量的分子量标准品。 在一个方面，分子量标准物被预染色。

    Method for treatment of a semisolid material
    5.
    发明申请
    Method for treatment of a semisolid material 审中-公开
    半固体材料的处理方法

    公开(公告)号：US20050034989A1

    公开(公告)日：2005-02-17

    申请号：US10861787

    申请日：2004-06-04

    申请人： Roumen Bogoev ,  Scott Whitney ,  Joseph Amshey

    发明人： Roumen Bogoev ,  Scott Whitney ,  Joseph Amshey

    IPC分类号： G01N33/48 ,  C07K1/34 ,  C12M1/00 ,  C12M1/33 ,  C12N15/09 ,  C12Q1/68 ,  G01N1/10 ,  G01N1/28 ,  G01L1/20

    CPC分类号： C07K1/34 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

    摘要： An apparatus and method for mincing a gel includes a gel mincing tube and a mesh material. The mesh material extends across the end of the tube. To subdivide a gel using the mincing apparatus, a gel is placed upon the mesh material in the mincing tube, the mincing tube, mesh material and the gel are spun in a centrifuge, forcing the gel through the mesh material so that the gel is subdivided into generally uniform smaller fragments. The mesh material may be secured to a tube in the form of a nesting tube. The nesting tube nests within the opening of a recovery vessel. The mesh material may be placed in series with a conditionally porous membrane in the nesting tube. Centrifuging the nesting tube and the recovery vessel subdivides gel material into fragments by forcing the gel through the mesh material. The gel subsequently falls upon the membrane, and may be treated on the membrane to extract or otherwise treat analytes in the gel material.

    摘要翻译： 用于切割凝胶的设备和方法包括凝胶切割管和网状材料。 网状材料延伸穿过管的端部。 为了使用切碎装置对凝胶进行细分，将凝胶放置在打孔管中的网状材料上，在离心机中将切细管，网状材料和凝胶纺丝，迫使凝胶通过网状材料，使凝胶细分 变成大致均匀的较小碎片。 网状材料可以以嵌套管的形式固定到管。 嵌套管嵌套在回收容器的开口内。 网状材料可以与嵌套管中的条状多孔膜串联放置。 将嵌套管和回收容器离心通过使凝胶通过网状材料将凝胶材料细分成碎片。 凝胶随后落在膜上，并且可以在膜上处理以提取或以其它方式处理凝胶材料中的分析物。

    Apparatus for conducting electrophoresis experiments
    7.
    发明授权
    Apparatus for conducting electrophoresis experiments 失效
    电泳实验装置

    公开(公告)号：US5888369A

    公开(公告)日：1999-03-30

    申请号：US828398

    申请日：1997-03-28

    申请人： Barbara L. Tippins ,  Roumen Bogoev ,  Douglas R. Levy

    发明人： Barbara L. Tippins ,  Roumen Bogoev ,  Douglas R. Levy

    IPC分类号： G01N27/447 ,  G01N27/22

    CPC分类号： G01N27/44708

    摘要： An apparatus of conducting electrophoresis experiments including a container for receiving an electrophoresis buffer and a buffer core assembly for holding gel-containing cassettes that are immersed in the buffer for molecular separation of an electrophoresis sample. The buffer core assembly defines a flow path for the electrophoresis buffer which can be circulated by means of a heat exchanger and a pump to effect controlled heat exchange between the buffer and the cassette surfaces thereby maintaining the gel and the electrophoresis process at a desirable temperature uniformly over the surfaces of the gel cassette. The buffer core assembly also has upper well for receiving a second electrically chargeable buffer in contact with the gel so as to impose an electric field on the gel for the electrophoresis in conjunction with the first buffer. The well is isolated from the first buffer to prevent mixing of the buffers and to reduce the risk of an electrical short.

    摘要翻译： 一种进行电泳实验的装置，包括用于接收电泳缓冲液的容器和用于保持浸入缓冲液中用于分离分离电泳样品的凝胶含有盒的缓冲芯组件。 缓冲芯组件限定了用于电泳缓冲液的流动路径，其可以通过热交换器和泵循环，以实现缓冲器和盒表面之间的受控热交换，从而将凝胶和电泳过程保持在期望的温度下均匀 在凝胶盒的表面上。 缓冲芯组件还具有用于接收与凝胶接触的第二可充电缓冲液的上孔，以便在与第一缓冲液结合的电泳上在凝胶上施加电场。 该井与第一缓冲液隔离，以防止缓冲液混合并降低电气短路的风险。

    Alkyl phosphine detergent products and extraction processes
    8.
    发明申请
    Alkyl phosphine detergent products and extraction processes 审中-公开
    烷基膦洗涤剂产品和提取工艺

    公开(公告)号：US20060183889A1

    公开(公告)日：2006-08-17

    申请号：US11324816

    申请日：2006-01-04

    申请人： Roumen Bogoev

    发明人： Roumen Bogoev

    IPC分类号： C07K1/10

    CPC分类号： C07F9/5304 ,  C07K1/145

    摘要： Provided herein are compositions and kits comprising alkylphosphine oxide detergents, and methods for the use thereof for the extraction of component molecules from cells. These compositions, kits and methods can provide efficient extraction of cellular components in a mild environment. While applicable to cell-associated molecules generally, the methods and compositions described herein are sometimes used to extract and purify polypeptides from cells or cell extracts, while maintaining a mild pH.

    摘要翻译： 本文提供了包含烷基氧化膦洗涤剂的组合物和试剂盒，以及用于从细胞中提取组分分子的方法。 这些组合物，试剂盒和方法可以在温和的环境中提供细胞成分的有效提取。 通常适用于细胞相关分子时，本文所述的方法和组合物有时用于从细胞或细胞提取物中提取和纯化多肽，同时保持温和的pH。