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1.发明申请Process for conjugating biomolecules to hydrophobic membrane-incorporated molecules 审中-公开将生物分子与疏水性膜结合分子结合的方法公开(公告)号：US20110217459A1
公开(公告)日：2011-09-08
申请号：US11974970
申请日：2007-10-16
申请人： Basil I. Swanson , Clifford J. Unkefer , Jurgen G. Schmidt , Scott M. Reed , John E. Shively , Lin Li
发明人： Basil I. Swanson , Clifford J. Unkefer , Jurgen G. Schmidt , Scott M. Reed , John E. Shively , Lin Li
IPC分类号： B05D5/06
CPC分类号： G01N33/5432
摘要： A process is provided of conjugating a recognition element such as a biomolecule to a hydrophobic multifunctional linker molecule by incorporating a multifunctional linker molecule including one or more anchoring groups, a reporter group and a reactive site thereon into a membrane, and, reacting the membrane including the incorporated multifunctional linker molecule with a pre-selected recognition element to form a covalently bound recognition element-multifunctional linker molecule-membrane assembly. Also, a chemical assembly suitable for subsequent covalent attachment of a recognition element is provided such assembly including a multifunctional linker molecule including one or more anchoring groups, a reporter group, and a hydrophilic spacer terminated by a reactive group capable of subsequent covalent bonding, the one or more anchoring groups incorporated in a membrane.
摘要翻译： 提供了通过将包括一个或多个锚定基团，报道基团和反应位点的多官能连接分子掺入到膜中，使识别元件例如生物分子与疏水多功能连接分子结合的方法， 所引入的多功能连接分子具有预选的识别元件以形成共价结合的识别元件 - 多功能连接子分子 - 膜组件。 此外，提供适于随后共价连接识别元件的化学组合物，其包括包含一个或多个锚定基团的多官能连接分子，报道基团和由能够随后共价键合的反应性基团封端的亲水性间隔基 一个或多个结合在膜中的锚定基团。
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公开(公告)号：US20100099831A1
公开(公告)日：2010-04-22
申请号：US11590109
申请日：2006-10-30
IPC分类号： C08L69/00
CPC分类号： G01N33/54353 , B01J19/0046 , B01J2219/00497 , B01J2219/005 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731
摘要： The present invention is directed to a method of forming an immobilized chemical moiety, such as a trifunctional chemical moiety that includes membrane-anchoring functionalities, on a solid support for use in automated chemical synthesis of a recognition molecule. The invention is further directed to a method of easily and efficiently synthesizing chemical moieties such as those used for biosensor applications. The invention is further directed to a composition, i.e., a solid support having a trifunctional chemical moiety linked thereto, that can be readily and easily used to generate biological molecules for applications, such as biosensor applications.
摘要翻译： 本发明涉及在用于识别分子的自动化学合成中的固体支持物上形成固定的化学部分例如包括膜锚定功能的三官能化学部分的方法。 本发明进一步涉及容易且有效地合成化学部分的方法，例如用于生物传感器应用的那些。 本发明进一步涉及一种组合物，即具有与其连接的三官能化学部分的固体支持物，其可以容易地和容易地用于产生用于应用的生物分子，例如生物传感器应用。
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公开(公告)号：US06627396B1
公开(公告)日：2003-09-30
申请号：US09699225
申请日：2000-10-27
IPC分类号： G01N3353
CPC分类号： C12Q1/701 , C12Q1/34 , G01N33/56983 , G01N2333/11 , Y10S435/808 , Y10S435/817 , Y10S436/805 , Y10S436/811 , Y10S436/815 , Y10T436/13 , Y10T436/200833
摘要： A sensor for the detection of tetrameric multivalent neuraminidase within a sample is disclosed, where a positive detection indicates the presence of a target virus within the sample. Also disclosed is a trifunctional composition of matter including a trifunctional linker moiety with groups bonded thereto including (a) an alkyl chain adapted for attachment to a substrate, (b) a fluorescent moiety capable of generating a fluorescent signal, and (c) a recognition moiety having a spacer group of a defined length thereon, the recognition moiety capable of binding with tetrameric multivalent neuraminidase.
摘要翻译： 公开了用于检测样品中四聚体多价神经氨酸酶的传感器，其中阳性检测表明样品中存在目标病毒。 还公开了一种三官能组合物，其包括具有与其键合的基团的三官能连接体部分，其包含（a）适于连接到底物的烷基链，（b）能够产生荧光信号的荧光部分，和（c）识别 具有其上限定长度的间隔基的部分，所述识别部分能够与四聚体多价神经氨酸酶结合。
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4.发明申请Robust multidentate ligands for diagnosis and anti-viral drugs for influenza and related viruses 审中-公开用于流感和相关病毒诊断和抗病毒药物的强大的多齿配体公开(公告)号：US20080194801A1
公开(公告)日：2008-08-14
申请号：US11728334
申请日：2007-03-26
IPC分类号： C07H15/00
CPC分类号： C07H15/00
摘要： Design and synthesis of a novel library of compounds comprising a spacer with an attachment element on one terminus and a recognition element on the other terminus is presented. The library of compounds can be attached to a solid support and used as an integral component of sensors and biosensors or the library of compounds can be used as antiviral drugs or to isolate pathogens from complex mixtures for further analysis.
摘要翻译： 提出了一种新型化合物文库的设计和合成，其包括在一个末端具有连接元件的间隔物和另一个末端的识别元件。 化合物库可以连接到固体支持物上，并用作传感器和生物传感器的组成部分，或者化合物文库可以用作抗病毒药物或从复杂混合物中分离病原体进行进一步分析。
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公开(公告)号：US07018792B2
公开(公告)日：2006-03-28
申请号：US11132130
申请日：2005-05-17
IPC分类号： G01N33/53 , G01N33/533 , C12Q1/70
CPC分类号： C12Q1/701 , C12Q1/34 , G01N33/56983 , G01N2333/11 , Y10S435/808 , Y10S435/817 , Y10S436/805 , Y10S436/811 , Y10S436/815 , Y10T436/13 , Y10T436/200833
摘要： A sensor for the detection of tetrameric multivalent neuraminidase within a sample is disclosed, where a positive detection indicates the presence of a target virus within the sample. Also disclosed is a trifunctional composition of matter including a trifunctional linker moiety with groups bonded thereto including (a) an alkyl chain adapted for attachment to a substrate, (b) a fluorescent moiety capable of generating a fluorescent signal, and (c) a recognition moiety having a spacer group of a defined length thereon, the recognition moiety capable of binding with tetrameric multivalent neuraminidase.
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公开(公告)号：US08759008B2
公开(公告)日：2014-06-24
申请号：US12777837
申请日：2010-05-11
IPC分类号： G01N33/53
CPC分类号： G01N33/54393 , G01N33/551 , Y10T428/31663
摘要： The present invention provides a composite material including a substrate having an oxide surface, and, a continuous monolayer on the oxide surface, the monolayer including a silicon atom from a trifunctional alkyl/alkenyl/alkynyl silane group that attaches to the oxide surface, an alkyl/alkenyl/alkynyl portion of at least three carbon atoms, a polyalkylene glycol spacer group, and either a reactive site (e.g., a recognition ligand) or a site resistant to non-specific binding (e.g., a methoxy or the like) at the terminus of each modified SAM. The present invention further provides a sensor element, a sensor array and a method of sensing, each employing the composite material. Patterning is also provided together with backfilling to minimize non-specific binding.
摘要翻译： 本发明提供一种复合材料，其包括具有氧化物表面的基材和在氧化物表面上的连续单层，所述单层包含与氧化物表面连接的三官能烷基/烯基/炔基硅烷基团的硅原子，烷基 至少三个碳原子的链烯基/炔基部分，聚亚烷基二醇间隔基，以及反应性位点（例如，识别配体）或对非特异性结合（例如，甲氧基等）具有抗性的位点 每个修饰的SAM的末端。 本发明还提供了各自采用复合材料的传感器元件，传感器阵列和感测方法。 还提供图案化以及回填以最小化非特异性结合。
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公开(公告)号：US20100267170A1
公开(公告)日：2010-10-21
申请号：US12777837
申请日：2010-05-11
IPC分类号： G01N33/544
CPC分类号： G01N33/54393 , G01N33/551 , Y10T428/31663
摘要： The present invention provides a composite material including a substrate having an oxide surface, and, a continuous monolayer on the oxide surface, the monolayer including a silicon atom from a trifunctional alkyl/alkenyl/alkynyl silane group that attaches to the oxide surface, an alkyl/alkenyl/alkynyl portion of at least three carbon atoms, a polyalkylene glycol spacer group, and either a reactive site (e.g., a recognition ligand) or a site resistant to non-specific binding (e.g., a methoxy or the like) at the terminus of each modified SAM. The present invention further provides a sensor element, a sensor array and a method of sensing, each employing the composite material. Patterning is also provided together with backfilling to minimize non-specific binding.
摘要翻译： 本发明提供一种复合材料，其包括具有氧化物表面的基材和在氧化物表面上的连续单层，所述单层包含与氧化物表面连接的三官能烷基/烯基/炔基硅烷基团的硅原子，烷基 至少三个碳原子的链烯基/炔基部分，聚亚烷基二醇间隔基，以及反应性位点（例如，识别配体）或对非特异性结合（例如，甲氧基等）具有抗性的位点 每个修饰的SAM的末端。 本发明还提供了各自采用复合材料的传感器元件，传感器阵列和感测方法。 还提供图案化以及回填以最小化非特异性结合。
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公开(公告)号：US06893814B2
公开(公告)日：2005-05-17
申请号：US10651141
申请日：2003-08-28
IPC分类号： C12Q1/34 , G01N33/569 , G01N33/53 , G01N33/543
CPC分类号： C12Q1/701 , C12Q1/34 , G01N33/56983 , G01N2333/11 , Y10S435/808 , Y10S435/817 , Y10S436/805 , Y10S436/811 , Y10S436/815 , Y10T436/13 , Y10T436/200833
摘要： A sensor for the detection of tetrameric multivalent neuraminidase within a sample is disclosed, where a positive detection indicates the presence of a target virus within the sample. Also disclosed is a trifunctional composition of matter including a trifunctional linker moiety with groups bonded thereto including (a) an alkyl chain adapted for attachment to a substrate, (b) a fluorescent moiety capable of generating a fluorescent signal, and (c) a recognition moiety having a spacer group of a defined length thereon, the recognition moiety capable of binding with tetrameric multivalent neuraminidase.
摘要翻译： 公开了用于检测样品中四聚体多价神经氨酸酶的传感器，其中阳性检测表明样品中存在目标病毒。 还公开了一种三官能组合物，其包括具有与其键合的基团的三官能连接体部分，其包含（a）适于连接到底物的烷基链，（b）能够产生荧光信号的荧光部分，和（c）识别 具有其上限定长度的间隔基的部分，所述识别部分能够与四聚体多价神经氨酸酶结合。
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9.发明授权Planar optical waveguide based sandwich assay sensors and processes for the detection of biological targets including protein markers, pathogens and cellular debris 有权基于平面光波导的夹层测定传感器和用于检测生物靶标（包括蛋白质标记物，病原体和细胞碎片）的方法公开(公告)号：US07541197B2
公开(公告)日：2009-06-02
申请号：US11172246
申请日：2005-06-29
IPC分类号： G01N33/551
CPC分类号： G01N21/648 , B01J2219/00497 , B01J2219/00527 , B01J2219/00576 , B01J2219/00605 , B01J2219/00612 , B01J2219/0063 , B01J2219/00637 , B01J2219/00725 , B01J2219/00743 , B82Y15/00 , B82Y30/00 , C12Q1/6825 , G01N21/6428 , G01N33/54373 , G01N2610/00 , Y10S436/805
摘要： An assay element is described including recognition ligands bound to a film on a single mode planar optical waveguide, the film from the group of a membrane, a polymerized bilayer membrane, and a self-assembled monolayer containing polyethylene glycol or polypropylene glycol groups therein and an assay process for detecting the presence of a biological target is described including injecting a biological target-containing sample into a sensor cell including the assay element, with the recognition ligands adapted for binding to selected biological targets, maintaining the sample within the sensor cell for time sufficient for binding to occur between selected biological targets within the sample and the recognition ligands, injecting a solution including a reporter ligand into the sensor cell; and, interrogating the sample within the sensor cell with excitation light from the waveguide, the excitation light provided by an evanescent field of the single mode penetrating into the biological target-containing sample to a distance of less than about 200 nanometers from the waveguide thereby exciting the fluorescent-label in any bound reporter ligand within a distance of less than about 200 nanometers from the waveguide and resulting in a detectable signal.
摘要翻译： 描述了测定元件，其包括在单模平面光波导上与膜结合的识别配体，来自膜组的膜，聚合双层膜和其中含有聚乙二醇或聚丙二醇基团的自组装单层，以及 描述了用于检测生物靶的存在的测定方法，包括将包含生物靶标的样品注入包含测定元件的传感器单元中，其中识别配体适于结合选定的生物学靶标，将样品保持在传感器单元内以供时间 足以在样品内的选定生物靶和识别配体之间发生结合，将包含报道配体的溶液注入到传感器细胞中; 并且使用来自波导的激发光来询问传感器单元内的样本，由单一模式的消逝场提供的激发光穿透到含生物靶标的样品中，距离波导的距离小于约200纳米，由此令人兴奋 在距离波导小于约200纳米的距离内的任何结合的报道配体中的荧光标记，并产生可检测的信号。
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公开(公告)号：US5418058A
公开(公告)日：1995-05-23
申请号：US132391
申请日：1993-10-04
申请人： DeQuan Li , Basil I. Swanson
发明人： DeQuan Li , Basil I. Swanson
CPC分类号： G01N33/0047 , G01N29/022 , G01N2291/0256 , G01N2291/0423 , G01N2291/0426 , G01N2291/0427 , Y10T428/251 , Y10T428/254 , Y10T428/269
摘要： An article of manufacture is provided including a substrate having an oxide surface layer and a selective thin film of a cyclodextrin derivative chemically bound upon said substrate, said film is adapted for the inclusion of a selected organic compound therewith. Such an article can be either a chemical sensor capable of detecting a resultant mass change from inclusion of the selected organic compound or a chemical separator capable of reversibly selectively separating a selected organic compound.
摘要翻译： 提供了一种制造物品，其包括具有氧化物表面层和化学键合在所述基底上的环糊精衍生物的选择性薄膜的基底，所述膜适合于包含选定的有机化合物。 这样的制品可以是能够检测所选质量变化的化学传感器，也可以是选择性地选择性分离选择的有机化合物的化学分离器。
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