公开(公告)号：US20210115489A1

公开(公告)日：2021-04-22

申请号：US17046610

申请日：2019-02-26

申请人： KIKKOMAN CORPORATION

发明人： Yosuke MASAKARI ,  Naoya TOTSUKA ,  Yasutomo SHINOHARA

IPC分类号： C12Q1/00 ,  C07C211/55 ,  H01M4/90 ,  H01M8/16

摘要： Provided is a novel mediator. The present invention relates to a novel mediator, and an electrode modifying agent and an electron transfer promoting agent comprising the mediator, an electrode, a battery, a composition, and an enzyme sensor comprising the electrode modifying agent or the electron transfer promoting agent, and a method using any of these.

公开(公告)号：US20210317463A1

公开(公告)日：2021-10-14

申请号：US17319362

申请日：2021-05-13

申请人： KIKKOMAN CORPORATION

发明人： Keiichi ICHIKAWA ,  Yasutomo SHINOHARA ,  Seiichi HARA ,  Keiko KUROSAWA ,  Yumiko YAMASHITA ,  Michiaki YAMASHITA

IPC分类号： C12N15/63 ,  C12N15/09 ,  C12N9/02 ,  C12N9/88 ,  C12N1/14 ,  C12P17/10

摘要： The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields as compared with a conventional technology, and, therefore, enables selenoneine production on an industrial scale. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant that has a gene encoding an enzyme of (1) below introduced therein and that can overexpress the introduced gene, to obtain selenoneine.
(1) An enzyme that catalyzes a reaction in which hercynylselenocysteine is produced from histidine and selenocysteine in the presence of S-adenosylmethionine and iron (II).

公开(公告)号：US20200063143A1

公开(公告)日：2020-02-27

申请号：US16612413

申请日：2018-05-11

申请人： KIKKOMAN CORPORATION ,  NAGASAKI UNIVERSITY

发明人： Yasuko ARAKI ,  Yasutomo SHINOHARA ,  Kiyoshi KITA

IPC分类号： C12N15/52 ,  C12P17/04 ,  C12P7/24 ,  C12P7/26 ,  C12N1/14

摘要： The problem to be solved by the present invention is to provide a method of producing isoprenoids including ascofuranone, ilicicolin A, and ascochlorin and derivatives thereof in a high yield as compared to the conventional art, which method enables industrial-scale production of isoprenoids. The problem can be solved by a method of producing isoprenoids such as ascofuranone, ilicicolin A, and ascochlorin, including using a transformant obtained by transformation with biosynthetic genes for ascofuranone, ilicicolin A, or ascochlorin or a knockout organism for these genes to obtain isoprenoids such as ascofuranone, ilicicolin A, and ascochlorin.

公开(公告)号：US20180237815A1

公开(公告)日：2018-08-23

申请号：US15750792

申请日：2016-06-17

申请人： KIKKOMAN CORPORATION

发明人： Keiichi ICHIKAWA ,  Yasutomo SHINOHARA ,  Seiichi HARA ,  Keiko KUROSAWA ,  Yumiko YAMASHITA ,  Michiaki YAMASHITA

IPC分类号： C12P17/10 ,  C12R1/685 ,  C12N1/14 ,  C12N15/09 ,  C12R1/665 ,  C12R1/69

CPC分类号： C12P17/10 ,  C12N1/14 ,  C12N15/09 ,  C12R1/66 ,  C12R1/665 ,  C12R1/685 ,  C12R1/69

摘要： The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields as compared with a conventional technology, and, therefore, enables selenoneine production on an industrial scale. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant that has a gene encoding an enzyme of (1) below introduced therein and that can overexpress the introduced gene, to obtain selenoneine. (1) An enzyme that catalyzes a reaction in which hercynylselenocysteine is produced from histidine and selenocysteine in the presence of S-adenosylmethionine and iron (II).