公开(公告)号：US11285492B2

公开(公告)日：2022-03-29

申请号：US16393967

申请日：2019-04-25

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sun Mi Lee ,  Yong-Sang Ryu ,  Eui-Sang Yu ,  Jiwon Kim ,  Ja Kyong Ko ,  Gyeongtaek Gong ,  Chulki Kim ,  Jae Hun Kim

IPC: B03C5/00 ,  C12P7/64 ,  C12P7/649

Abstract: Disclosed herein are a screening method of high-efficiency biofuel-producing strains by a dielectrophoretic method using vertical nano-gap electrodes and a producing method of biofuel from the screened strains.

公开(公告)号：US11932895B2

公开(公告)日：2024-03-19

申请号：US17849571

申请日：2022-06-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Hyun Ju Oh ,  Youngsoon Um ,  Sun Mi Lee ,  Gyeongtaek Gong ,  Ja Kyong Ko

IPC: C12P7/06 ,  C12P7/16 ,  C12R1/145

CPC classification number: C12P7/06 ,  C12P7/16 ,  C12R2001/145

Abstract: The present disclosure relates to a composition for preparing hexanol or butanol and a method for preparing hexanol or butanol using ethanol and synthesis gas, wherein the composition according to an aspect of the present disclosure is a medium composition containing ethanol as an active ingredient, and by culturing a strain producing hexanol or butanol after inoculating with a medium containing the composition and supplying synthesis gas, hexanol or butanol can be prepared economically using inexpensive synthesis gas, and hexanol or butanol can be prepared with high efficiency by focusing the flow of a carbon source consumed in a fermentation process to the production of hexanol or butanol.

公开(公告)号：US11319556B2

公开(公告)日：2022-05-03

申请号：US16551335

申请日：2019-08-26

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sun Mi Lee ,  Phuong Nguyen Hoang Tran ,  Youngsoon Um ,  Gyeongtaek Gong ,  Ja Kyong Ko ,  Yeonjung Lee

IPC: C12N15/90 ,  C12N1/16 ,  C12N15/81 ,  C12P7/06 ,  C12P7/16 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/92 ,  C12N15/11 ,  C12N9/22

Abstract: The present specification relates to a transformed yeast strain capable of simultaneously utilizing xylose and glucose as carbon sources, a preparation method thereof and a biofuel production method using the same. The transformed yeast strain transforms a wild-type yeast strain incapable of using xylose as a carbon source and simultaneously convert glucose and xylose, thereby enabling high yield production of a biofuel. The economics and sustainability of the biofuel and biomaterial production processes can be highly enhanced by providing a strain which can easily be converted to a strain capable of producing a biofuel/material in a high yield through an additional modification.

公开(公告)号：US10913942B2

公开(公告)日：2021-02-09

申请号：US16204020

申请日：2018-11-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sun Mi Lee ,  Sangdo Yook ,  Youngsoon Um ,  Gyeongtaek Gong ,  Jiwon Kim

IPC: C12N9/92 ,  C12N1/16 ,  C12P7/64 ,  C12N9/12 ,  C12N15/01 ,  C12N15/52 ,  C07K14/39

Abstract: Disclosed herein are a yeast strain capable of utilizing xylose as a carbon source and a method for producing lipids using the same. The yeast strain is obtained by adaptively evolving a wild-type yeast strain which cannot utilize xylose as a carbon source so that it can produce high density lipids and then transforming the adaptively evolved strain to obtain the ability to metabolize xylose. Since the strain does not have the xylose metabolic pathway based on oxidoreductase, it can produce biodiesel and biomaterials based on lipid and lignocellulosic biomass at a high yield without a problem of cofactor imbalance and can greatly improve the economic feasibility and sustainability of the production processes of biodiesel and biomaterials.

公开(公告)号：US10036046B2

公开(公告)日：2018-07-31

申请号：US15299188

申请日：2016-10-20

Applicant: Korea Institute of Science and Technology

Inventor： Han Min Woo ,  Hyun Jeong Lee ,  Youngsoon Um ,  Gyeong Taek Gong ,  Sun Mi Lee ,  Yunje Kim

IPC: C12N1/20 ,  C12N15/70 ,  C12P7/64 ,  C07H21/04 ,  C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52

CPC classification number: C12P7/649 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52 ,  C12P7/6463 ,  C12Y101/01001 ,  C12Y401/01001 ,  Y02E50/13

Abstract: The present disclosure discloses a transformed Synechococcus elongatus strain capable of producing biodiesel directly from carbon dioxide and a method for producing biodiesel and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce biodiesel in large scale using carbon dioxide as a carbon source. The Synechococcus elongatus strain is environment-friendly because it can be used to remove or reduce carbon dioxide in the atmosphere. The strain of the present disclosure is advantageous in that it can produce biodiesel in large scale because it grows faster and exhibits excellent carbon dioxide fixation capability as compared to other photosynthetic microorganisms.

公开(公告)号：US12228496B2

公开(公告)日：2025-02-18

申请号：US17083299

申请日：2020-10-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Myung-Suk Chun ,  Sun Mi Lee ,  Jae Hun Kim ,  Chansung Park

IPC: G01N15/14 ,  B01L3/00 ,  G01N21/64

Abstract: Provided is an apparatus for cell particle sorting based on microfluidic-chip flow, by using a design in which Dean flow focusing occurring in a spiral channel and hydrodynamic filtration are coupled. The apparatus comprises a first substrate including a spiral channel having an inner surface and an outer surface based on a radius of curvature, a sample solution inlet, a medium inlet, and a spiral inner-outlet and a spiral outer-outlet both for discharging the particles, and a second substrate including a main channel in which the sample solution discharged from the first substrate and passing through an inter-substrate way flows and a cut-off width WC is set, a side channel allowing a medium introduced into the medium inlet to flow to focus the sample solution on a sidewall of the main channel, a plurality of branch channels connected to the sidewall of main channel and configured to receive the particles from the main channel, a main channel outlet, and at least one branch channel outlet.

公开(公告)号：US20200149071A1

公开(公告)日：2020-05-14

申请号：US16551335

申请日：2019-08-26

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sun Mi Lee ,  Phuong Nguyen Hoang Tran ,  Youngsoon Um ,  Gyeongtaek Gong ,  Ja Kyong Ko ,  Yeonjung Lee

IPC: C12N15/90 ,  C12N9/92 ,  C12N9/12 ,  C12N9/10 ,  C12P7/06 ,  C12N15/81 ,  C12N1/16 ,  C12N15/11 ,  C12N9/22 ,  C12N9/04 ,  C12N9/02

Abstract: The present specification relates to a transformed yeast strain capable of simultaneously utilizing xylose and glucose as carbon sources, a preparation method thereof and a biofuel production method using the same. The transformed yeast strain transforms a wild-type yeast strain incapable of using xylose as a carbon source and simultaneously convert glucose and xylose, thereby enabling high yield production of a biofuel. The economics and sustainability of the biofuel and biomaterial production processes can be highly enhanced by providing a strain which can easily be converted to a strain capable of producing a biofuel/material in a high yield through an additional modification.

公开(公告)号：US10155964B2

公开(公告)日：2018-12-18

申请号：US15357815

申请日：2016-11-21

Applicant: Korea Institute of Science and Technology

Inventor： Han Min Woo ,  Sun-young Choi ,  Youngsoon Um ,  Gyeong Taek Gong ,  Sun Mi Lee ,  Yunje Kim

IPC: C12N9/04 ,  C12N9/10 ,  C12N9/90 ,  C12P5/00 ,  C12N15/52

Abstract: The present specification discloses a transformed Synechococcus elongatus strain which may directly produce squalene from carbon dioxide, and a method for producing squalene and a method for removing carbon dioxide, using the same. In an aspect, the strain may produce squalene using carbon dioxide as a carbon source. The Synechococcus elongatus strain is economically efficient because a high-value added squalene is produced using light and carbon dioxide present in the atmosphere as a carbon source, and the method for producing squalene is eco-friendly because the strain may be utilized to remove or reduce carbon dioxide in the atmosphere by using microorganisms. The strain of the present disclosure may produce only squalene, which is a desired target material with high purity, and has an advantage in that squalene may be continuously mass-produced.

公开(公告)号：US09719095B2

公开(公告)日：2017-08-01

申请号：US14950425

申请日：2015-11-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min Woo ,  Eun Mi Kim ,  Youngsoon Um ,  Gyeongtaek Gong ,  Sun Mi Lee ,  Yunje Kim

IPC: C12N15/52 ,  C12N15/72 ,  C12N15/71 ,  C12N9/10 ,  C12N9/88 ,  C12N9/04 ,  C12N9/12 ,  C12N9/90 ,  C12N15/70

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12N15/70 ,  C12N15/71 ,  C12N15/72 ,  C12Y101/01034 ,  C12Y203/01026 ,  C12Y205/01 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y401/01033 ,  C12Y401/03 ,  C12Y503/03002

Abstract: Disclosed herein are an expression vector capable of expressing myrcene, an Escherichia coli strain transformed with the vector and having improved capability of producing myrcene and a method for producing myrcene and a method for recycling glycerol using the same. In an aspect, the transformed Escherichia coli strain of the present disclosure can produce myrcene with high purity on a large scale using glycerol or glucose as a carbon source. Also, the Escherichia coli strain of the present disclosure is economical and environment-friendly because it can produce high value-added myrcene using waste glycerol as a carbon source. In addition, the strongly volatile myrcene can be produced and isolated at the same time.