公开(公告)号：US10155964B2

公开(公告)日：2018-12-18

申请号：US15357815

申请日：2016-11-21

Applicant: Korea Institute of Science and Technology

Inventor： Han Min Woo ,  Sun-young Choi ,  Youngsoon Um ,  Gyeong Taek Gong ,  Sun Mi Lee ,  Yunje Kim

IPC: C12N9/04 ,  C12N9/10 ,  C12N9/90 ,  C12P5/00 ,  C12N15/52

Abstract: The present specification discloses a transformed Synechococcus elongatus strain which may directly produce squalene from carbon dioxide, and a method for producing squalene and a method for removing carbon dioxide, using the same. In an aspect, the strain may produce squalene using carbon dioxide as a carbon source. The Synechococcus elongatus strain is economically efficient because a high-value added squalene is produced using light and carbon dioxide present in the atmosphere as a carbon source, and the method for producing squalene is eco-friendly because the strain may be utilized to remove or reduce carbon dioxide in the atmosphere by using microorganisms. The strain of the present disclosure may produce only squalene, which is a desired target material with high purity, and has an advantage in that squalene may be continuously mass-produced.

公开(公告)号：US09719095B2

公开(公告)日：2017-08-01

申请号：US14950425

申请日：2015-11-24

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min Woo ,  Eun Mi Kim ,  Youngsoon Um ,  Gyeongtaek Gong ,  Sun Mi Lee ,  Yunje Kim

IPC: C12N15/52 ,  C12N15/72 ,  C12N15/71 ,  C12N9/10 ,  C12N9/88 ,  C12N9/04 ,  C12N9/12 ,  C12N9/90 ,  C12N15/70

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12N15/70 ,  C12N15/71 ,  C12N15/72 ,  C12Y101/01034 ,  C12Y203/01026 ,  C12Y205/01 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y401/01033 ,  C12Y401/03 ,  C12Y503/03002

Abstract: Disclosed herein are an expression vector capable of expressing myrcene, an Escherichia coli strain transformed with the vector and having improved capability of producing myrcene and a method for producing myrcene and a method for recycling glycerol using the same. In an aspect, the transformed Escherichia coli strain of the present disclosure can produce myrcene with high purity on a large scale using glycerol or glucose as a carbon source. Also, the Escherichia coli strain of the present disclosure is economical and environment-friendly because it can produce high value-added myrcene using waste glycerol as a carbon source. In addition, the strongly volatile myrcene can be produced and isolated at the same time.

公开(公告)号：US10036046B2

公开(公告)日：2018-07-31

申请号：US15299188

申请日：2016-10-20

Applicant: Korea Institute of Science and Technology

Inventor： Han Min Woo ,  Hyun Jeong Lee ,  Youngsoon Um ,  Gyeong Taek Gong ,  Sun Mi Lee ,  Yunje Kim

IPC: C12N1/20 ,  C12N15/70 ,  C12P7/64 ,  C07H21/04 ,  C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52

CPC classification number: C12P7/649 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52 ,  C12P7/6463 ,  C12Y101/01001 ,  C12Y401/01001 ,  Y02E50/13

Abstract: The present disclosure discloses a transformed Synechococcus elongatus strain capable of producing biodiesel directly from carbon dioxide and a method for producing biodiesel and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce biodiesel in large scale using carbon dioxide as a carbon source. The Synechococcus elongatus strain is environment-friendly because it can be used to remove or reduce carbon dioxide in the atmosphere. The strain of the present disclosure is advantageous in that it can produce biodiesel in large scale because it grows faster and exhibits excellent carbon dioxide fixation capability as compared to other photosynthetic microorganisms.

公开(公告)号：US09441230B2

公开(公告)日：2016-09-13

申请号：US14637355

申请日：2015-03-03

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Han Min Woo ,  Youngsoon Um ,  Yunje Kim ,  Gyeong Taek Gong ,  Jung Seok Lee

IPC: C12N15/77 ,  C12N15/72

CPC classification number: C12N15/77 ,  C12N15/72

Abstract: Disclosed is a shuttle vector that can be used for Corynebacterium and E. coli, containing: a repressor selected from a group consisting of a lacI repressor and a tetR repressor; a promoter selected from a group consisting of a trc promoter, a tetA promoter and a LacUV5 promoter; a replication origin pBL1 derived from Corynebacterium glutamicum; and a replication origin ColE1 of E. coli. A host cell transformed with the shuttle vector can effectively produce industrially useful substances. Also, the shuttle vector may be used to easily insert various combinations of target genes and, as a result, a variety of vectors can be prepared effectively.

Abstract translation: 公开了可用于棒状杆菌和大肠杆菌的穿梭载体，其包含：选自lacI阻遏物和tetR阻遏物的阻遏物; 选自由trc启动子，tetA启动子和LacUV5启动子组成的组的启动子; 衍生自谷氨酸棒杆菌的复制起点pBL1; 和大肠杆菌的复制起点ColE1。 用穿梭载体转化的宿主细胞可以有效地产生工业上有用的物质。 此外，穿梭载体可以用于容易地插入靶基因的各种组合，结果可以有效地制备各种载体。

公开(公告)号：US10087471B2

公开(公告)日：2018-10-02

申请号：US15092829

申请日：2016-04-07

Applicant: Korea Institute of Science and Technology

Inventor： Youngsoon Um ,  Yunje Kim ,  Kyung Min Lee ,  Han Min Woo ,  Gyeongtaek Gong ,  Ki Yeon Kim

IPC: C12P7/02 ,  C12P7/52 ,  C12P19/02 ,  C12P19/12

Abstract: The present disclosure relates to a hydrolysate of a mixture of lignocellulosic biomass and seaweed biomass. By mixing seaweed biomass with lignocellulosic biomass and then preparing a hydrolysate, lignocellulosic biomass-derived acetic acid is consumed together with seaweed biomass-derived mannitol. As a result, high sugar productivity can be maintained while reducing fermentation inhibitors. Because the present disclosure can solve the problem of lignocellulosic biomass of decreased fermentation efficiency due to lignocellulose-derived fermentation inhibitors and the problem of seaweed biomass of very low productivity in spite of long fermentation time, the hydrolysate according to the present disclosure may be used to produce biofuels and biochemicals economically.

公开(公告)号：US09719097B2

公开(公告)日：2017-08-01

申请号：US14860373

申请日：2015-09-21

Applicant: Korea Institute of Science and Technology

Inventor： Han Min Woo ,  Jun Won Chwa ,  Youngsoon Um ,  Gyeong Taek Gong ,  Yunje Kim

IPC: C07H21/02 ,  C07H21/04 ,  A61K48/00 ,  C12N15/74

CPC classification number: C12N15/74

Abstract: The present disclosure discloses a vector that can be used for both cyanobacteria and E. coli, which contains, sequentially, a pUC replication origin as a replication origin; a spectinomycin-resistant gene as a selection marker; and a promoter selected from a group consisting of a trc promoter, a tetA promoter or a modified tetA promoter, a BAD promoter and a cbbL promoter. An industrially useful substance may be produced effectively using a host cell transformed with the vector. Also, the vector may be used to insert a variety of target genes through simple combination and, as a result, various vectors can be prepared effectively.

公开(公告)号：US09657318B2

公开(公告)日：2017-05-23

申请号：US14874040

申请日：2015-10-02

Applicant: Korea Institute of Science and Technology

Inventor： Youngsoon Um ,  Yunje Kim ,  Dongjin Suh ,  Kyung Min Lee ,  Jeong Myeong Ha

IPC: C12P7/10 ,  C12P7/52 ,  C12P7/64 ,  C12P19/02 ,  C12P19/14 ,  C12P7/16

CPC classification number: C12P7/52 ,  C12P7/10 ,  C12P7/16 ,  C12P7/64 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12P2203/00 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/343

Abstract: Provided is a method for preparing a lignocellulosic biomass hydrolysate having toxicity reduced or removed and being used for fermentation by microorganisms, comprising the steps of: pretreating, hydrolyzing, and using an electrochemical detoxification method.