公开(公告)号：US10087472B2

公开(公告)日：2018-10-02

申请号：US14889543

申请日：2014-05-02

Applicant: Korea Research Institute of Bioscience and Biotechnology

Inventor： Hong-Weon Lee ,  Jung Oh Ahn ,  Joon Ki Jung ,  Hee-Ju Ko ,  Sun-Joo Park ,  Chun Sug Kim ,  Hyeok Won Lee ,  Eun Gyo Lee ,  Joo Hwan Lee

IPC: C12P7/02 ,  C12N1/20 ,  C12N15/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C07H21/04 ,  C12P13/00 ,  C07D223/10 ,  C12N15/52 ,  C12P17/10

Abstract: The present invention relates to a method for preparing a recombinant microorganism simultaneously comprising genes encoding enzymes used in the biosynthesis pathway of 6-aminocaproic acid, which is a precursor of caprolactam, biosynthesizing 6-aminocaproic acid from the microorganism, and producing the same so as to synthesize caprolactam.

公开(公告)号：US09902969B2

公开(公告)日：2018-02-27

申请号：US15319273

申请日：2015-06-16

Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

Inventor： Eun Gyo Lee ,  Hong-Weon Lee ,  Shin Young Kang ,  Yeon-Gu Kim ,  Joon Ki Jung ,  Jungoh Ahn ,  Seung Hee Kang ,  Chun Sug Kim ,  Hyeok Won Lee ,  Jin Gyeom Lee

IPC: C12N15/85 ,  C12Q1/68 ,  C07K14/435 ,  C07K16/00

CPC classification number: C12N15/85 ,  C07K14/43595 ,  C07K16/00 ,  C07K2317/14 ,  C12N15/63 ,  C12N2840/203 ,  C12N2840/60 ,  C12N2840/85 ,  C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6897

Abstract: The present invention relates to a new gene fragment derived from Chinese hamster ovary (CHO) cell for enhancement of recombinant protein expression in animal cells and a use thereof. It has been found that using the vector comprising a gene fragment of the present invention enhances the expression of a target protein in animal cells. Accordingly, the vector comprising a gene fragment of the present invention could be usefully used in the production of biopharmaceuticals such as therapeutic antibodies, etc.

公开(公告)号：US20160257976A1

公开(公告)日：2016-09-08

申请号：US14889543

申请日：2014-05-02

Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

Inventor： Hong-Weon Lee ,  Jung Oh Ahn ,  Joon Ki Jung ,  Hee-Ju Ko ,  Sun-Joo Park ,  Chun Sug Kim ,  Hyeok Won Lee ,  Eun Gyo Lee ,  Joo Hwan Lee

IPC: C12P13/00 ,  C12N15/70 ,  C07D223/10

CPC classification number: C12P13/005 ,  C07D223/10 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12P17/10

Abstract: The present invention relates to a method for preparing a recombinant microorganism simultaneously comprising genes encoding enzymes used in the biosynthesis pathway of 6-aminocaproic acid, which is a precursor of caprolactam, biosynthesizing 6-aminocaproic acid from the microorganism, and producing the same so as to synthesize caprolactam.

Abstract translation: 本发明涉及一种同时制备重组微生物的方法，该方法同时包含编码在6-氨基己酸的生物合成途径中使用的酶的基因，该氨基己酸是己内酰胺的前体，从微生物中合成6-氨基己酸， 合成己内酰胺。