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    NOVEL LOW-MOLECULAR-COMPOUND FOR IMPROVING PRODUCTION, MAINTENANCE AND PROLIFERATION OF PLURIPOTENT STEM CELLS, COMPOSITION COMPRISING THE SAME, AND CULTURE METHOD
    2.
    发明申请
    NOVEL LOW-MOLECULAR-COMPOUND FOR IMPROVING PRODUCTION, MAINTENANCE AND PROLIFERATION OF PLURIPOTENT STEM CELLS, COMPOSITION COMPRISING THE SAME, AND CULTURE METHOD 有权
    用于改善生物，维持和增殖细胞干细胞的新型低分子化合物，包含其的组合物和培养方法

    公开(公告)号：US20150159142A1

    公开(公告)日：2015-06-11

    申请号：US14358672

    申请日：2012-11-08

    Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

    Inventor： Yee Sook Cho ,  Kyeong Lee ,  Jung Woon Lee

    IPC: C12N5/074 ,  C07D209/24 ,  C07D405/12

    CPC classification number: C12N5/0696 ,  C07D209/18 ,  C07D209/20 ,  C07D209/24 ,  C07D405/12 ,  C12N5/0606 ,  C12N2500/02 ,  C12N2500/30 ,  C12N2501/115 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/09

    Abstract: According to the present invention, when the novel low-molecular-weight compound RSC-133 is added in a culture process for producing reprogrammed pluripotent stem cells from human differentiated cells, it can increase the efficiency of reprogramming and can significantly reduce the time required for the induction of reprogramming. Particularly, the novel compound RSC-133 can substitute for c-Myc acting as both a reprogramming factor and an oncogenic factor, and it can effectively increase the efficiency of reprogramming in both normal oxygen culture conditions and hypoxic culture conditions. In addition, RSC-133 can inhibit the induction of aging occurring in the reprogramming process, exhibits the effect of promoting cell proliferation, and induces epigenetic activation to improve culture conditions for induction of reprogramming. The present invention will contribute to optimizing a process of producing induced pluripotent stem cells from a small amount of patient-specific somatic cells obtained from various sources, and thus it will significantly improve a process of developing clinically applicable personalized stem cell therapy agents and new drugs and will facilitate the practical use of these agents and drugs. In addition, the novel low-molecular-weight compound RSC-133 can provide a cell culture medium effective for maintaining the undifferentiated state of human embryonic stem cells that are typical pluripotent stem cells. The medium composition containing RSC-133 can effectively induce the proliferation of human embryonic stem cells in an undifferentiated state and can be effectively used for the development of a system for culturing large amounts of embryonic stem cells.

    Abstract translation: 根据本发明，当在用于从人分化细胞产生重编程多能干细胞的培养方法中添加新的低分子量化合物RSC-133时，可以提高重编程的效率，并且可以显着减少所需的时间 诱导重编程。 特别是，新型化合物RSC-133可以代替c-Myc，既可作为重编程因子又可作为致癌因子，可以有效提高正常氧培养条件和缺氧培养条件下重编程效率。 另外，RSC-133可以抑制重编程过程中发生的老化诱导，表现出促进细胞增殖的作用，诱导表观遗传活化，改善诱导重编程的培养条件。 本发明将有助于从从各种来源获得的少量患者特异性体细胞中优化产生诱导性多能干细胞的方法，并且因此将显着改善开发临床适用的个体化干细胞治疗剂和新药的方法 并将促进这些药剂和药物的实际应用。 另外，新型低分子量化合物RSC-133可提供有效维持作为典型多能干细胞的人胚胎干细胞的未分化状态的细胞培养基。 含有RSC-133的培养基组合物可以有效地诱导未分化状态的人类胚胎干细胞的增殖，可有效地用于培养大量胚胎干细胞的系统。

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