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    Amplified nucleic acid detection method and detection device
    1.
    发明授权
    Amplified nucleic acid detection method and detection device 有权

    公开(公告)号：US10829805B2

    公开(公告)日：2020-11-10

    申请号：US15887334

    申请日：2018-02-02

    申请人： Kaneka Corporation

    发明人： Koji Takahashi ,  Shigehiko Miyamoto ,  Takaaki Jikihara ,  Jun Tomono

    IPC分类号： C12P19/34 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6832 ,  G01N33/543

    摘要： An object of the invention is to provide a nucleic acid detection method which takes advantage of the high specificity of hybridization techniques, reduces the time length and the number of steps required for detection of PCR products, and allows for easy and highly accurate detection by visual observation without the need of special equipment; and a nucleic acid detection device or kit. The invention provides a method for detecting a target nucleic acid in a sample, which includes performing amplification of the target nucleic acid sequence to synthesize an amplification product having a partially double-stranded structure where a single-stranded region is added to each end of the target sequence, and hybridizing a nucleic acid sequence bound to a development medium and a nucleic acid sequence labeled with a labeling compound with the single-stranded regions of the amplification product to form a sandwich hybridization complex; and a detection device thereof.

    RNA PREPARATION METHOD
    4.
    发明申请
    RNA PREPARATION METHOD 有权
    RNA制备方法

    公开(公告)号：US20150376600A1

    公开(公告)日：2015-12-31

    申请号：US14437936

    申请日：2013-10-25

    申请人： Kaneka Corporation ,  BIOCOSM INC.

    发明人： Sotaro Sano ,  Shigehiko Miyamoto ,  Jun Tomono ,  Hajime Hiratsuka

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1003 ,  C07H1/06 ,  C07H1/08 ,  C12Q1/6806 ,  C12Q2527/125

    摘要： An object of the present invention is to provide a technique for preparing RNA ready for an enzymatic reaction more easily than conventional techniques. The present invention provides a reagent for RNA extraction from a biological sample which contains an alkali metal salt and a surfactant.

    摘要翻译： 本发明的目的是提供一种比常规技术更容易制备准备用于酶促反应的RNA的技术。 本发明提供了从含有碱金属盐和表面活性剂的生物样品中提取RNA的试剂。

    Method for detecting nucleic acid, and device or kit
    6.
    发明授权
    Method for detecting nucleic acid, and device or kit 有权

    公开(公告)号：US10073040B2

    公开(公告)日：2018-09-11

    申请号：US13768143

    申请日：2013-02-15

    申请人： Kaneka Corporation

    发明人： Shigehiko Miyamoto ,  Tomohisa Kato ,  Koji Takahashi ,  Jun Tomono

    IPC分类号： G01N21/78 ,  C12Q1/6816 ,  G01N33/52 ,  G01N33/53

    CPC分类号： G01N21/78 ,  C12Q1/6816 ,  G01N33/52 ,  G01N33/5308 ,  C12Q2563/173 ,  C12Q2537/101 ,  C12Q2563/137

    摘要： A method and a device or kit for detecting a nucleic acid, which enable simple and precise visual detection of a nucleic acid amplified by an nucleic acid amplification method, without necessity of special devices are provided. The method for detecting a nucleic acid in a sample comprises: contacting a sample with a dye to react with each other; and observing a substance produced by the reaction with visible light, and evaluating the presence or absence of a nucleic acid by eye. The device or kit for detecting a nucleic acid in a sample comprises: a carrier that holds a dye which can bind to a nucleic acid; a path for passing a sample through the carrier; and an evaluation part for observing a substance produced by the reaction between the sample and the dye with visible light, and evaluating the presence or absence of a nucleic acid by eye.

    NUCLEIC ACID DETECTION METHOD, AND DEVICE AND KIT FOR USE IN SAME
    9.
    发明申请
    NUCLEIC ACID DETECTION METHOD, AND DEVICE AND KIT FOR USE IN SAME 审中-公开
    核酸检测方法，以及用于其的装置和试剂盒

    公开(公告)号：US20160209332A1

    公开(公告)日：2016-07-21

    申请号：US15001811

    申请日：2016-01-20

    申请人： Kaneka Corporation

    发明人： Shigehiko Miyamoto ,  Jun Tomono ,  Koji Takahashi ,  Sotaro Sano ,  Takaaki Jikihara

    IPC分类号： G01N21/78 ,  C12Q1/68

    CPC分类号： G01N21/78 ,  C08F222/10 ,  C12Q1/6806 ,  C12Q1/6846 ,  C12Q1/6848 ,  G01N21/03 ,  Y10T436/143333 ,  C12Q2547/101 ,  C12Q2563/103 ,  C12Q2563/173

    摘要： The present invention aims to solve problems in the analysis of amplified nucleic acids, i.e., cost, workability, and contamination and pollution of samples. In the present invention, a sample containing a nucleic acid and a reagent for detecting the nucleic acid are mixed in a closed system. In this regard, the nucleic acid is amplified in the same closed system, and then mixed with the detecting reagent without opening the system. In order to carry out this method, for example, a device that includes the detecting reagent shielded by a coating material or the like is used.

    摘要翻译： 本发明旨在解决扩增核酸分析中的问题，即成本，可加工性以及样品的污染和污染。 在本发明中，含有核酸和检测核酸的试剂的样品在封闭系统中混合。 在这方面，核酸在相同的封闭系统中扩增，然后与检测试剂混合而不打开系统。 为了实施该方法，例如，使用包含被涂料等遮蔽的检测试剂的装置。