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    AMPLIFIED NUCLEIC ACID DETECTION METHOD AND DETECTION DEVICE
    2.
    发明申请
    AMPLIFIED NUCLEIC ACID DETECTION METHOD AND DETECTION DEVICE 审中-公开

    公开(公告)号：US20180155771A1

    公开(公告)日：2018-06-07

    申请号：US15887334

    申请日：2018-02-02

    申请人： Kaneka Corporation

    发明人： Koji TAKAHASHI ,  Shigehiko Miyamoto ,  Takaaki Jikihara ,  Jun Tomono

    IPC分类号： C12Q1/6834 ,  C12Q1/686 ,  G01N33/543

    摘要： An object of the invention is to provide a nucleic acid detection method which takes advantage of the high specificity of hybridization techniques, reduces the time length and the number of steps required for detection of PCR products, and allows for easy and highly accurate detection by visual observation without the need of special equipment; and a nucleic acid detection device or kit. The invention provides a method for detecting a target nucleic acid in a sample, which includes performing amplification of the target nucleic acid sequence to synthesize an amplification product having a partially double-stranded structure where a single-stranded region is added to each end of the target sequence, and hybridizing a nucleic acid sequence bound to a development medium and a nucleic acid sequence labeled with a labeling compound with the single-stranded regions of the amplification product to form a sandwich hybridization complex; and a detection device thereof.

    Amplified nucleic acid detection method and detection device
    3.
    发明授权
    Amplified nucleic acid detection method and detection device 有权

    公开(公告)号：US10829805B2

    公开(公告)日：2020-11-10

    申请号：US15887334

    申请日：2018-02-02

    申请人： Kaneka Corporation

    发明人： Koji Takahashi ,  Shigehiko Miyamoto ,  Takaaki Jikihara ,  Jun Tomono

    IPC分类号： C12P19/34 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6832 ,  G01N33/543

    摘要： An object of the invention is to provide a nucleic acid detection method which takes advantage of the high specificity of hybridization techniques, reduces the time length and the number of steps required for detection of PCR products, and allows for easy and highly accurate detection by visual observation without the need of special equipment; and a nucleic acid detection device or kit. The invention provides a method for detecting a target nucleic acid in a sample, which includes performing amplification of the target nucleic acid sequence to synthesize an amplification product having a partially double-stranded structure where a single-stranded region is added to each end of the target sequence, and hybridizing a nucleic acid sequence bound to a development medium and a nucleic acid sequence labeled with a labeling compound with the single-stranded regions of the amplification product to form a sandwich hybridization complex; and a detection device thereof.

    Test device
    6.
    发明授权
    Test device 有权

    公开(公告)号：US11154856B2

    公开(公告)日：2021-10-26

    申请号：US16259277

    申请日：2019-01-28

    申请人： KANEKA CORPORATION

    发明人： Sotaro Sano ,  Shigehiko Miyamoto ,  Takaaki Jikihara

    IPC分类号： B01L3/00 ,  G01N30/91 ,  G01N30/92 ,  G01N30/90 ,  C12M1/00 ,  C12Q1/6844

    摘要： A test device includes a housing and a lid. The housing encloses an internal space, has a hole supporting a container accommodating liquid, and includes a perforation/incision part. The lid covers a hole-formed part of the housing. The housing and/or the lid includes a guide that guides the housing and the lid, so that the lid can migrate from the first position to the second position while covering the hole-formed part of the housing. In the first position, the lid covers the hole-formed part of the housing and the container and the container is not incised. During the migration of the lid from the first position to the second position, the container is pushed toward the perforation/incision part by the lid, and the container is incised to leak liquid into the internal space.

    TEST DEVICE
    7.
    发明申请
    TEST DEVICE 审中-公开

    公开(公告)号：US20190168209A1

    公开(公告)日：2019-06-06

    申请号：US16259277

    申请日：2019-01-28

    申请人： KANEKA CORPORATION

    发明人： Sotaro Sano ,  Shigehiko Miyamoto ,  Takaaki Jikihara

    IPC分类号： B01L3/00 ,  G01N30/91 ,  C12Q1/6844

    摘要： A test device includes a housing and a lid. The housing encloses an internal space, has a hole supporting a container accommodating liquid, and includes a perforation/incision part. The lid covers a hole-formed part of the housing. The housing and/or the lid includes a guide that guides the housing and the lid, so that the lid can migrate from the first position to the second position while covering the hole-formed part of the housing. In the first position, the lid covers the hole-formed part of the housing and the container and the container is not incised. During the migration of the lid from the first position to the second position, the container is pushed toward the perforation/incision part by the lid, and the container is incised to leak liquid into the internal space.

    NUCLEIC ACID DETECTION METHOD, AND DEVICE AND KIT FOR USE IN SAME
    10.
    发明申请
    NUCLEIC ACID DETECTION METHOD, AND DEVICE AND KIT FOR USE IN SAME 审中-公开
    核酸检测方法，以及用于其的装置和试剂盒

    公开(公告)号：US20160209332A1

    公开(公告)日：2016-07-21

    申请号：US15001811

    申请日：2016-01-20

    申请人： Kaneka Corporation

    发明人： Shigehiko Miyamoto ,  Jun Tomono ,  Koji Takahashi ,  Sotaro Sano ,  Takaaki Jikihara

    IPC分类号： G01N21/78 ,  C12Q1/68

    CPC分类号： G01N21/78 ,  C08F222/10 ,  C12Q1/6806 ,  C12Q1/6846 ,  C12Q1/6848 ,  G01N21/03 ,  Y10T436/143333 ,  C12Q2547/101 ,  C12Q2563/103 ,  C12Q2563/173

    摘要： The present invention aims to solve problems in the analysis of amplified nucleic acids, i.e., cost, workability, and contamination and pollution of samples. In the present invention, a sample containing a nucleic acid and a reagent for detecting the nucleic acid are mixed in a closed system. In this regard, the nucleic acid is amplified in the same closed system, and then mixed with the detecting reagent without opening the system. In order to carry out this method, for example, a device that includes the detecting reagent shielded by a coating material or the like is used.

    摘要翻译： 本发明旨在解决扩增核酸分析中的问题，即成本，可加工性以及样品的污染和污染。 在本发明中，含有核酸和检测核酸的试剂的样品在封闭系统中混合。 在这方面，核酸在相同的封闭系统中扩增，然后与检测试剂混合而不打开系统。 为了实施该方法，例如，使用包含被涂料等遮蔽的检测试剂的装置。