公开(公告)号：US5843709A

公开(公告)日：1998-12-01

申请号：US466961

申请日：1995-06-06

申请人： Karl-Dieter Entian ,  Friedrich Gotz ,  Norbert Schnell ,  Johannes Augustin ,  Germar Engelke ,  Ralf Rosenstein ,  Cortina Kaletta ,  Cora Klein ,  Bernd Wieland ,  Thomas Kupke ,  Gunther Jung ,  Roland Kellner

发明人： Karl-Dieter Entian ,  Friedrich Gotz ,  Norbert Schnell ,  Johannes Augustin ,  Germar Engelke ,  Ralf Rosenstein ,  Cortina Kaletta ,  Cora Klein ,  Bernd Wieland ,  Thomas Kupke ,  Gunther Jung ,  Roland Kellner

IPC分类号： C07K1/113 ,  C07K14/31 ,  C12N9/00 ,  C12N15/00 ,  C12N15/52 ,  C12P21/02 ,  C12P21/06 ,  A61K38/00 ,  C12N1/00

CPC分类号： C07K14/31 ,  C07K1/113 ,  C12N15/00 ,  C12N15/52 ,  C12N9/00 ,  C12P21/02 ,  C07K2319/00 ,  C07K2319/02 ,  Y10S435/882 ,  Y10S530/825

摘要： A bacterial host is described which is transformed by a plasmid coding for a polypeptide precursor wherein the host comprises a multi-enzyme complex capable of reacting with the expressed polypeptide precursor to produce a polypeptide comprising at least one dehydroamino acid and/or at least one lanthione bridge. A process for producing a polypeptide comprising at least one dehydroamino acid and/or at least one lanthione bridge, such as gallidermin, is also described. A plasmid capable of transforming a bacterial host is additionally described. Also disclosed are recombinant DNA molecules which specify Epi B, Epi C, Epi D, Epi P and Epi Q, enzymes which are involved in the biosynthesis of lantibiotic epidermin.

摘要翻译： 描述了由编码多肽前体的质粒转化的细菌宿主，其中宿主包含能与表达的多肽前体反应的多酶复合物，以产生包含至少一个脱氢氨基酸和/或至少一个叶酸的多肽 桥。 还描述了产生包含至少一种脱氢氨基酸和/或至少一种叶黄素桥的多肽的方法，例如gallidermin。 另外描述了能够转化细菌宿主的质粒。 还公开了指定Epi B，Epi C，Epi D，Epi P和Epi Q的重组DNA分子，其涉及羊毛硫抗生素表皮生物合成的酶。

公开(公告)号：US5837485A

公开(公告)日：1998-11-17

申请号：US392625

申请日：1995-02-22

申请人： Karl-Dieter Entian ,  Friedrich Gotz ,  Norbert Schnell ,  Johannes Augustin ,  Germar Engelke ,  Ralf Rosenstein ,  Cortina Kaletta ,  Cora Klein ,  Bernd Wieland ,  Thomas Kupke ,  Gunther Jung ,  Roland Kellner

发明人： Karl-Dieter Entian ,  Friedrich Gotz ,  Norbert Schnell ,  Johannes Augustin ,  Germar Engelke ,  Ralf Rosenstein ,  Cortina Kaletta ,  Cora Klein ,  Bernd Wieland ,  Thomas Kupke ,  Gunther Jung ,  Roland Kellner

IPC分类号： C07K1/113 ,  C07K14/31 ,  C12N9/00 ,  C12N15/00 ,  C12N15/52 ,  C12P21/02 ,  C12N15/63

CPC分类号： C07K14/31 ,  C07K1/113 ,  C12N15/00 ,  C12N15/52 ,  C12N9/00 ,  C12P21/02 ,  C07K2319/00 ,  C07K2319/02 ,  Y10S435/882 ,  Y10S530/825

摘要： A bacterial host is described which is transformed by a plasmid coding for a polypeptide precursor wherein the host comprises a multi-enzyme complex capable of reacting with the expressed polypeptide precursor to produce a polypeptide comprising at least one dehydroamino acid and/or at least one lanthionine bridge. A process for producing a polypeptide comprising at least one dehydroamino acid and/or at least one lanthionine bridge, such as gallidermin, is also described. A plasmid capable of transforming a bacterial host is additionally described. Also disclosed are recombinant DNA molecules which specify Epi B, Epi C, Epi D, Epi P and Epi Q, enzymes which are involved in the biosynthesis of lantibiotic epidermin.

摘要翻译： 描述了由编码多肽前体的质粒转化的细菌宿主，其中所述宿主包含能够与表达的多肽前体反应以产生包含至少一种脱氢氨基酸和/或至少一种甲硫氨酸的多肽的多酶复合物 桥。 还描述了用于产生包含至少一种脱氢氨基酸和/或至少一种甲硫氨酸桥（例如gallidermin）的多肽的方法。 另外描述了能够转化细菌宿主的质粒。 还公开了指定Epi B，Epi C，Epi D，Epi P和Epi Q的重组DNA分子，其涉及羊毛硫抗生素表皮生物合成的酶。

公开(公告)号：US5962253A

公开(公告)日：1999-10-05

申请号：US645193

申请日：1996-05-13

申请人： Thomas Kupke ,  Friedrich Gotz ,  Christoph Kempter ,  Gunther Jung

发明人： Thomas Kupke ,  Friedrich Gotz ,  Christoph Kempter ,  Gunther Jung

IPC分类号： C07K14/31 ,  C12N9/88 ,  C12P21/04 ,  C12P21/06 ,  C12P33/20 ,  C12N1/20 ,  C12N9/04

CPC分类号： C12P21/06 ,  C07K14/31 ,  C12N9/88 ,  C07K2319/00

摘要： A method is described for oxidatively decarboxylating a peptide, comprising combining a peptide with EpiD, wherein the peptide comprises at its carboxy terminus the amino acid sequence X.sub.1 X.sub.2 X.sub.3 X.sub.4 X.sub.5 X.sub.6 C, wherein X.sub.1, X.sub.2, X.sub.3 and X.sub.4 are any one of the twenty common amino acids, X.sub.5 is Tyr, Val, Met, Leu, Ile, Phe or Trp, and X.sub.6 is Cys, Ala, Ser, Val, or Thr, whereby the oxidative decarboxylation of the peptide occurs.

摘要翻译： 描述了一种用于氧化脱羧肽的方法，包括将肽与EpiD组合，其中肽在其羧基末端包含氨基酸序列X1X2X3X4X5X6C，其中X1，X2，X3和X4是二十个常见氨基酸中的任何一个，X5 是Tyr，Val，Met，Leu，Ile，Phe或Trp，X6是Cys，Ala，Ser，Val或Thr，由此发生肽的氧化脱羧作用。