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18 条记录 (耗时 0.039 秒)

    Polypeptide
    5.
    发明申请
    Polypeptide 审中-公开
    多肽

    公开(公告)号：US20060018997A1

    公开(公告)日：2006-01-26

    申请号：US10886527

    申请日：2004-07-07

    申请人： Karsten Kragh ,  Bo Sorensen

    发明人： Karsten Kragh ,  Bo Sorensen

    IPC分类号： A21D8/02 ,  C12N1/21

    CPC分类号： C12N9/2417 ,  A21D8/042

    摘要： We describe a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 161 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. The PS4 variant polypeptide may further comprise one or more further mutations at a position selected from the group consisting of: 121 and 223, preferably 121F, 121Y, 121W, 223E and 223K, more preferably G121F, G121Y, G121W, G223E and/or G223K, or the group consisting of: 134, 141, 157, 223, 307, 334, 33 and 34, preferably G134R, A141P, I157L, G223A, H307L, S334P, N33Y and D34N. In preferred embodiments, the PS4 variant polypeptide further comprises a mutation at position 87, preferably G87S, a mutation at position 178, preferably L178F, and/or a mutation at position 179, preferably A179T.

    摘要翻译： 我们描述了一种包含PS4变体多肽的食品添加剂，其中PS4变体多肽可衍生自具有非麦芽糖外切酶活性的亲本多肽，其中PS4变体多肽包含参考位置编号在161位置的氨基酸取代 的SEQ ID NO：1所示的假单胞菌糖酵母外切酶序列.PS4变体多肽可以在选自121和223，优选121F，121Y，121W，223E和223K的位置进一步包含一个或多个另外的突变 ，更优选G121F，G121Y，G121W，G223E和/或G223K，或由134,141,157,223,307,334,33和34，优选G134R，A141P，I157L，G223A，H307L，S334P， N33Y和D34N。 在优选的实施方案中，PS4变体多肽还包含位置87，优选G87S，178位突变，优选L178F和/或179位突变，优选A179T的突变。

    Polypeptide
    7.
    发明申请
    Polypeptide 审中-公开
    多肽

    公开(公告)号：US20070141693A1

    公开(公告)日：2007-06-21

    申请号：US11483220

    申请日：2006-07-07

    申请人： Casper Berg ,  Patrick Maria Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Kellet-Smith ,  Karsten Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Sorensen ,  Charlotte Thoudahl

    发明人： Casper Berg ,  Patrick Maria Derkx ,  Carol Fioresi ,  Gijsbert Gerritse ,  Anja Kellet-Smith ,  Karsten Kragh ,  Wei Liu ,  Andrew Shaw ,  Bo Sorensen ,  Charlotte Thoudahl

    IPC分类号： C12N9/32 ,  C12N1/21

    CPC分类号： A21D8/042 ,  A23L33/18 ,  C12N9/2411 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/2425 ,  C12N9/2428

    摘要： The invention provides for a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity which may be selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.

    摘要翻译： 本发明提供可衍生自具有淀粉酶活性的亲本多肽的PS4变体多肽，其可以选自： 146，157，161，178，179，223，229，272，303，307，309和334; （b）包含位置33,34,121,134,141,145,146,157,178,179,223,229,272,303,307和334的氨基酸突变的多肽; （c）包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和（d）包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 参考如SEQ ID NO：1所示的假单胞菌属酵母内切酶序列的位置编号，这种多肽作为食品或饲料添加剂的使用，以及编码其的核酸。