Microscope and observation method
    5.
    发明授权
    Microscope and observation method 有权
    显微镜和观察方法

    公开(公告)号:US09001321B2

    公开(公告)日:2015-04-07

    申请号:US13578164

    申请日:2011-02-08

    IPC分类号: G01J3/44 G02B21/00 G01N21/65

    摘要: Provided is a microscope and an observation method which can improve spatial resolution. A microscope according to an aspect of the invention includes a laser light source (10), an objective lens (16) that focuses light from the laser light source on a sample, and a detector (22) that detects the laser light as signal light from a sample (17) when the sample (17) is irradiated with the laser light. The light is applied to the sample with an intensity changed to obtain a nonlinear region where intensities of the light and the signal light have a nonlinear relation due to occurrence of saturation or nonlinear increase of the signal light when the light has a maximum intensity, and the detector (22) detects the signal light according to the intensity of the laser light to perform observation based on a saturation component or a nonlinear increase component of the signal light.

    摘要翻译: 提供了可以提高空间分辨率的显微镜和观察方法。 根据本发明的一个方面的显微镜包括激光源(10),将来自激光光源的光聚焦在样本上的物镜(16)以及检测器(22),其将激光检测为信号光 来自样品(17)当样品(17)被激光照射时。 将光以强度变化的方式施加到样品,以获得非线性区域,其中当光具有最大强度时,光和信号光的强度由于饱和度的发生或信号光的非线性增加而具有非线性关系,以及 检测器(22)根据激光的强度检测信号光,以基于信号光的饱和分量或非线性增加分量进行观察。

    MICROSCOPE AND OBSERVATION METHOD
    6.
    发明申请
    MICROSCOPE AND OBSERVATION METHOD 有权
    显微镜和观察方法

    公开(公告)号:US20120307238A1

    公开(公告)日:2012-12-06

    申请号:US13578164

    申请日:2011-02-08

    摘要: Provided is a microscope and an observation method which can improve spatial resolution. A microscope according to an aspect of the invention includes a laser light source (10), an objective lens (16) that focuses light from the laser light source on a sample, and a detector (22) that detects the laser light as signal light from a sample (17) when the sample (17) is irradiated with the laser light. The light is applied to the sample with an intensity changed to obtain a nonlinear region where intensities of the light and the signal light have a nonlinear relation due to occurrence of saturation or nonlinear increase of the signal light when the light has a maximum intensity, and the detector (22) detects the signal light according to the intensity of the laser light to perform observation based on a saturation component or a nonlinear increase component of the signal light.

    摘要翻译: 提供了可以提高空间分辨率的显微镜和观察方法。 根据本发明的一个方面的显微镜包括激光源(10),将来自激光光源的光聚焦在样本上的物镜(16)以及检测器(22),其将激光检测为信号光 来自样品(17)当样品(17)被激光照射时。 将光以强度变化的方式施加到样品,以获得非线性区域,其中当光具有最大强度时,光和信号光的强度由于饱和度的发生或信号光的非线性增加而具有非线性关系,以及 检测器(22)根据激光的强度检测信号光,以基于信号光的饱和分量或非线性增加分量进行观察。

    Pulse Laser Light Timing Adjusting Device, Adjusting Method, and Optical Microscope
    7.
    发明申请
    Pulse Laser Light Timing Adjusting Device, Adjusting Method, and Optical Microscope 审中-公开
    脉冲激光定时调节装置,调节方法和光学显微镜

    公开(公告)号:US20100232459A1

    公开(公告)日:2010-09-16

    申请号:US12227412

    申请日:2006-11-20

    IPC分类号: G02F1/35

    摘要: To provide a pulse laser light timing adjusting device capable of easily adjusting pulse laser light timing, a timing adjusting method, and an optical microscope. An optical microscope in accordance with the present invention includes mirror pairs 21 and 31 to generates the first timing adjusting optical beam in which the first pulse laser light ω1 is delayed from the second pulse laser light ω2 and the second timing adjusting optical beam in which the second pulse laser light ω2 is delayed from the first pulse laser light having a first wavelength from the optical beam extracted at the beam samplers 15 and 16, a first detector 23 and a second detector 33 to output a first detection signal and a second detection signal based on a nonlinear optical effect, and timing adjusting means 42 to adjust the timing based on the first detection signal and the second detection signal.

    摘要翻译: 提供能够容易地调整脉冲激光定时的脉冲激光定时调整装置,定时调整方法和光学显微镜。 根据本发明的光学显微镜包括镜对21和31,用于产生第一定时调整光束,其中第一脉冲激光光源ω1从第二脉冲激光光源ω2延迟,第二定时调整光束 第二脉冲激光器ω2从具有第一波长的第一脉冲激光从在光束采样器15和16处提取的光束被延迟,第一检测器23和第二检测器33输出第一检测信号和第二检测信号 以及基于第一检测信号和第二检测信号来调整定时的定时调整装置42。

    Fluorescence Microscope and Fluorescence Microscope Method
    8.
    发明申请
    Fluorescence Microscope and Fluorescence Microscope Method 有权
    荧光显微镜和荧光显微镜法

    公开(公告)号:US20080215272A1

    公开(公告)日:2008-09-04

    申请号:US11792304

    申请日:2005-10-14

    IPC分类号: G06F15/00

    摘要: To increase spatial resolution by observing a sample based on saturated fluorescence components. A fluorescence microscope according to the present invention includes: a laser light source 10 emitting laser light as excitation light; an objective lens 13 focusing the laser light and applying the focused laser light to a sample 14; a detector 22 detecting fluorescence generated in the sample 14 with the laser light; and a stage 15 scanning the sample 14 while moving the sample 14 relative to the laser light, wherein the laser light is applied to the sample with varying intensities such that saturation of fluorescence occurs at the maximum intensity of the laser light, and fluorescence is detected with the detector in accordance with intensity of the laser light, and the sample is observed based on the saturation components of fluorescence.

    摘要翻译: 通过观察基于饱和荧光成分的样品来增加空间分辨率。 根据本发明的荧光显微镜包括:发射激光作为激发光的激光光源10; 聚焦激光并将聚焦的激光施加到样品14的物镜13; 用激光检测样品14中产生的荧光的检测器22; 以及在样品14相对于激光移动的同时扫描样品14的阶段15,其中激光以不同的强度施加到样品,使得在激光的最大强度下发生荧光饱和,并且检测到荧光 与检测器根据激光的强度,并且基于荧光的饱和分量观察样品。

    Fluorescence microscope for which a sample is observed based on the saturation components of fluorescence and fluorescence microscopy method
    9.
    发明授权
    Fluorescence microscope for which a sample is observed based on the saturation components of fluorescence and fluorescence microscopy method 有权
    基于荧光和荧光显微镜法的饱和组分观察样品的荧光显微镜

    公开(公告)号:US07781711B2

    公开(公告)日:2010-08-24

    申请号:US11792304

    申请日:2005-10-14

    IPC分类号: G02B7/04

    摘要: To increase spatial resolution by observing a sample based on saturated fluorescence components. A fluorescence microscope according to the present invention includes: a laser light source 10 emitting laser light as excitation light; an objective lens 13 focusing the laser light and applying the focused laser light to a sample 14; a detector 22 detecting fluorescence generated in the sample 14 with the laser light; and a stage 15 scanning the sample 14 while moving the sample 14 relative to the laser light, wherein the laser light is applied to the sample with varying intensities such that saturation of fluorescence occurs at the maximum intensity of the laser light, and fluorescence is detected with the detector in accordance with intensity of the laser light, and the sample is observed based on the saturation components of fluorescence.

    摘要翻译: 通过观察基于饱和荧光成分的样品来增加空间分辨率。 根据本发明的荧光显微镜包括:发射激光作为激发光的激光光源10; 聚焦激光并将聚焦的激光施加到样品14的物镜13; 用激光检测样品14中产生的荧光的检测器22; 以及在样品14相对于激光移动的同时扫描样品14的阶段15,其中激光以不同的强度施加到样品,使得在激光的最大强度下发生荧光饱和,并且检测到荧光 与检测器根据激光的强度,并且基于荧光的饱和分量观察样品。

    Microscope
    10.
    发明授权
    Microscope 有权
    显微镜

    公开(公告)号:US09582088B2

    公开(公告)日:2017-02-28

    申请号:US14006500

    申请日:2012-03-22

    CPC分类号: G06F3/041 G02B21/36 H04N7/18

    摘要: A touch screen (60) disposed on a display surface of a display means (58) is used as an inputting means so that a variety of actions of a microscope can be controlled with sufficient ease and precision.A variety of actions are controlled based on an operator's various finger gestures used on the touch screen disposed on the display surface of the display means, for example, making contact by a finger (tap), making two consecutive contacts by a finger (double-tap), making contact by a finger, and moving the finger without releasing it (drag), making contact by a finger and maintaining the contact for a predetermined time or longer (touch-and-hold), making simultaneous contact by two fingers, and increasing spacing between the fingers (pinch-out) or decreasing the spacing (pinch-in), and making simultaneous contact by two fingers, and moving the fingers in parallel (double-drag).

    摘要翻译: 基于操作者在显示装置的显示表面上设置的触摸屏上使用的各种手指手势来控制各种动作,例如通过手指(抽头)进行接触,通过手指(双重)进行两次连续的接触, 点击),通过手指进行接触,并且移动手指而不释放(拖动),通过手指进行接触并将触点保持预定时间或更长时间(触摸并保持),同时通过两个手指接触, 并增加手指之间的间距(挤出)或减小间距(夹紧),并通过两个手指进行同时接触,并平行移动手指(双重拖动)。