公开(公告)号：US5597727A

公开(公告)日：1997-01-28

申请号：US245688

申请日：1994-05-18

申请人： Keiko Kohama ,  Kazuhisa Hatakeyama ,  Yasurou Kurusu ,  Hideaki Yukawa

发明人： Keiko Kohama ,  Kazuhisa Hatakeyama ,  Yasurou Kurusu ,  Hideaki Yukawa

IPC分类号： C12N1/21 ,  C12N9/88 ,  C12N15/09 ,  C12N15/69 ,  C12N15/77 ,  C12R1/13 ,  C12R1/15 ,  C07H21/04 ,  C12N15/00 ,  C12N15/74

CPC分类号： C12N9/88 ,  C12N15/69 ,  C12N15/77 ,  Y10S435/84 ,  Y10S435/843

摘要： The present invention relates to a DNA fragment which contains a gene responsible for the function of autonomous replication of plasmid in a Coryneform bacterium, said gene being obtained from plasmid pBY503 held in Brevibacterium stationis, and in which at least one point mutation capable of increasing the copy number of plasmid exists on said gene region. By using a Coryneform transformed with the vector constructed using said DNA fragment and an industrially useful gene such as aspartase gene or tryptophan synthase gene, production of the useful product occurs with higher efficiency than conventional methods.

摘要翻译： 本发明涉及一种DNA片段，其含有负责棒状杆菌型细菌质粒自主复制功能的基因，所述基因得自持有短杆菌属的质粒pBY503，其中能够增加至少一个能够增加 所述基因区上存在质粒的拷贝数。 通过使用由使用所述DNA片段构建的载体和工业上有用的基因如天冬氨酸酶基因或色氨酸合成酶基因转化的棒状细胞，有效产物的产生以比常规方法更高的效率发生。

公开(公告)号：US5153123A

公开(公告)日：1992-10-06

申请号：US257524

申请日：1988-10-14

申请人： Masato Terasawa ,  Terukazu Nara ,  Makiko Fukushima ,  Yukie Satoo ,  Mitsunobu Shimazu ,  Hideaki Yukawa ,  Yasurou Kurusu ,  Keiko Kohama

发明人： Masato Terasawa ,  Terukazu Nara ,  Makiko Fukushima ,  Yukie Satoo ,  Mitsunobu Shimazu ,  Hideaki Yukawa ,  Yasurou Kurusu ,  Keiko Kohama

IPC分类号： C12N15/52 ,  C12N15/77 ,  C12P13/08

CPC分类号： C12P13/08 ,  C12N15/52 ,  C12N15/77 ,  Y10S435/84

摘要： A method for producing L-threonine, which comprises subjecting at least L- or DL-aspartic acid or a salt thereof to enzymatic reaction according to the reaction system not accompanied with growth of microorganism cells in an aqueous solution in the presence of a microorganism and collecting L-threonine formed, wherein the microorganism is a biotin-requiring microorganism for the growth belonging to coryneform bacterium; a plasmid comprising a DNA fragment containing at least a gene encoding biosynthesis of threonine which can be expressed within a biotin-requiring microorganism cell for the growth belonging to coryneform bacterium and a DNA fragment containing a gene encoding autonomous replication within coryneform bacterium cell; and a biotin-requiring microorganism for the growth belonging to coryneform bacterium which has been transformed with the plasmid described above, both of which are employed in the present method.According to the present invention, L-threonine can be produced with good yield, and further since production management becomes extremely easy without requiring cumbersome operation such as sterilization of the medium, etc. as in the fermentation method, L-threonine can be produced inexpensively in industry.

公开(公告)号：US5185262A

公开(公告)日：1993-02-09

申请号：US473396

申请日：1990-02-01

申请人： Keiko Kohama ,  Miki Kobayashi ,  Yasurou Kurusu ,  Hideaki Yukawa ,  Makiko Fukushima

发明人： Keiko Kohama ,  Miki Kobayashi ,  Yasurou Kurusu ,  Hideaki Yukawa ,  Makiko Fukushima

IPC分类号： C12N9/88 ,  C12N15/68 ,  C12N15/77

CPC分类号： C12N9/88 ,  C12N15/68 ,  C12N15/77

摘要： A DNA fragment derived from plasmid pBY503 obtained from Brevibacterium stationis IF012144, said DNA fragment containing a gene which encodes for the function of maintaining a plasmid, capable of replicating and proliferating at least in a Coryneform bacteria of the genus Brevibacterium, stably in said bacteria; and a vector DNA capable of replicating and proliferating in a Coryneform bacteria into which the above DNA fragment is introduced.

摘要翻译： 从得自短杆菌IF012144的质粒pBY503衍生的DNA片段，所述DNA片段含有能够稳定地在短杆菌中至少在短杆菌属的棒状细菌中复制和增殖的能够维持质粒的功能的基因的基因; 以及能够在导入上述DNA片段的棒状细菌中复制和增殖的载体DNA。