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    Methods for producing L-amino acids
    5.
    发明授权
    Methods for producing L-amino acids 失效
    生产L-氨基酸的方法

    公开(公告)号：US07192747B2

    公开(公告)日：2007-03-20

    申请号：US10309089

    申请日：2002-12-04

    申请人： Yukiko Ono ,  Hisashi Yasueda ,  Yoshio Kawahara ,  Shinichi Sugimoto

    发明人： Yukiko Ono ,  Hisashi Yasueda ,  Yoshio Kawahara ,  Shinichi Sugimoto

    IPC分类号： C12P13/04 ,  C12P13/14 ,  C12P13/12

    CPC分类号： C12R1/26 ,  C12P13/04 ,  C12P13/08 ,  C12P13/12 ,  C12R1/01

    摘要： A methane-utilizing microorganism capable of producing L-amino acid, for example, bacteria belonging to type I, type X or type II in the taxonomic categorization methane-utilizing bacteria such as Methylomonas albus, Methylococcus capsulatus and Methylosinus trichosporium, is cultivated in a culture medium in contact with gas containing methane which is the main source of carbon, to allow the L-amino acid to be produced and accumulated in the medium, and the L-amino acid is collected from the medium.

    摘要翻译： 能够生产L-氨基酸的甲烷利用微生物，例如属于I型，X型或II型的细菌，在分类分类甲烷利用细菌如Methylomonas albus，Methylococcus capsulatus和Methylosinus trichosporium中培养在 与作为主要碳源的甲烷的气体接触的培养基，以使L-氨基酸在培养基中产生和积累，从培养基中收集L-氨基酸。

    Insertion sequence
    6.
    发明授权
    Insertion sequence 失效
    插入序列

    公开(公告)号：US06303381B1

    公开(公告)日：2001-10-16

    申请号：US09455921

    申请日：1999-12-07

    申请人： Yoshiya Gunji ,  Hisashi Yasueda ,  Yoshio Kawahara ,  Shinichi Sugimoto

    发明人： Yoshiya Gunji ,  Hisashi Yasueda ,  Yoshio Kawahara ,  Shinichi Sugimoto

    IPC分类号： C12N1500

    CPC分类号： C07K14/195 ,  C12N15/11 ,  C12N15/74 ,  C12N15/90

    摘要： A novel insertion sequence, which has been found in the sMMO gene coding for methane monooxygenase of methane-assimilating bacterium Methylococcus capsulatus NCIMB 11132 strain, and has inverted repeat sequences consisting of a sequence of the nucleotide numbers 5-19 of SEQ ID NO: 1 at the both ends, can be utilized as effective means for genetic analysis including creation of insertion mutant strains, gene mapping, promoter searching, insertion of genetic information into chromosomal DNA, disruption of specific gene and the like, or utilized for improving methane-assimilating bacteria by chromosomal genetic engineering techniques.

    摘要翻译： 已经在编码甲烷同化细菌甲壳螨NCIMB 11132株的甲烷单加氧酶的sMMO基因中发现了新的插入序列，并且具有由SEQ ID NO：1的核苷酸编号5-19的序列组成的反向重复序列 在两端都可以用作遗传分析的有效手段，包括插入突变菌株的创建，基因作图，启动子搜索，遗传信息插入染色体DNA，破坏特定基因等，或用于改善甲烷同化 细菌通过染色体遗传工程技术。