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公开(公告)号：US07855059B2
公开(公告)日：2010-12-21
申请号：US10490565
申请日：2002-06-04
申请人： Kevin S. Wenger , Maria Antonieta Caicedo , Stuart Michael Stocks , Swapnil Bhargava , Mark R. Marten
发明人： Kevin S. Wenger , Maria Antonieta Caicedo , Stuart Michael Stocks , Swapnil Bhargava , Mark R. Marten
IPC分类号： C12P1/00
摘要： A process for the production of a valuable compound, comprising the steps of a) fermentation of a filamentous bacterial or fungal strain (e.g. a Streptomyces strain or an Aspergillus strain) in a fermentation medium wherein a carbohydrate during fermentation is added in a cyclic pulse dosing/pause way, wherein the pulse dosing time is shorter than the pause time and b) recovery of the valuable compound from the fermentation broth.
摘要翻译： 一种制备有价值化合物的方法，包括以下步骤：a）在发酵培养基中发酵丝状细菌或真菌菌株（例如链霉菌属菌株或曲霉属菌株），其中发酵过程中的碳水化合物以循环脉冲给药 /暂停方式，其中脉冲给药时间比暂停时间短，b）从发酵液中回收有价值的化合物。
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公开(公告)号：US20120108798A1
公开(公告)日：2012-05-03
申请号：US13124255
申请日：2009-10-16
CPC分类号： C12P7/22 , C12P7/10 , C12P2201/00 , Y02E50/16 , Y02E50/343
摘要： The present invention is directed to a process of producing substantially pure lignin from lignocellulosic biomass, which comprises: pre-treating a lignocellulosic feedstock to produce a reactive lignin-carbohydrate mixture; biologically-reacting the carbohydrates in the mixture, separating remaining solids from the liquid fermentation products, and drying the resulting solids to yield a substantially pure lignin product. Optionally, the lignin product may be washed and subjected to a second hydrolysis step. Optionally, the lignin product may be further processed by hydrotreating and/or pyrolysis in order to yield desirable products such as fuel additives.
摘要翻译： 本发明涉及从木质纤维素生物质生产基本上纯的木质素的方法，其包括：预处理木质纤维素原料以产生反应性木质素 - 碳水化合物混合物; 使混合物中的碳水化合物生物反应，将剩余的固体与液体发酵产物分离，并干燥得到的固体，得到基本上纯的木质素产物。 任选地，可以洗涤木质素产物并进行第二水解步骤。 任选地，可以通过加氢处理和/或热解进一步处理木质素产物，以产生所需的产物，例如燃料添加剂。
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公开(公告)号：US08076109B2
公开(公告)日：2011-12-13
申请号：US13079100
申请日：2011-04-04
摘要： The present invention relates to processes for producing a fermentation product, such as ethanol, from milled starch-containing material comprising (a) saccharifying the milled starch-containing material with a glucoamylase having an amino acid sequence shown in SEQ ID NO: 2, or a glucoamylase being at least 70% identical thereto, at a temperature below the initial gelatinization temperature of said starch-containing material, (b) fermenting using a fermenting organism.
摘要翻译： 本发明涉及由含碾磨的含淀粉材料生产发酵产物如乙醇的方法，该方法包括：（a）用含有SEQ ID NO：2所示氨基酸序列的葡糖淀粉酶糖化含碾磨淀粉的材料，或 在低于所述含淀粉材料的初始糊化温度的温度下，（b）使用发酵生物发酵的葡糖淀粉酶与其至少70％相同。
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4.发明申请Yeast Expressing Saccharolytic Enzymes for Consolidated Bioprocessing Using Starch and Cellulose 审中-公开酵母表达糖酵解酶用于固体生物处理使用淀粉和纤维素公开(公告)号：US20130323822A1
公开(公告)日：2013-12-05
申请号：US13701652
申请日：2011-06-03
申请人： Elena Brevnova , John E. McBride , Erin Wiswall , Kevin S. Wenger , Nicky Caiazza , Heidi Hau , Aaron Argyros , Frank Agbogbo , Charles F. Rice , Trisha Barrett , John S. Bardsley , Abigail S. Foster , Anne K. Warner , Mark Mellon , Ryan Skinner , Indraneel Shikhare , Riaan Den Haan , Chhayal V. Gandhi , Alan Belcher , Vineet B. Rajgarhia , Allan C. Froehlich , Kristen M. Deleault , Emily Stonehouse , Shital A. Tripathi , Jennifer Gosselin , Yin-Ying Chiu , Haowen Xu
发明人： Elena Brevnova , John E. McBride , Erin Wiswall , Kevin S. Wenger , Nicky Caiazza , Heidi Hau , Aaron Argyros , Frank Agbogbo , Charles F. Rice , Trisha Barrett , John S. Bardsley , Abigail S. Foster , Anne K. Warner , Mark Mellon , Ryan Skinner , Indraneel Shikhare , Riaan Den Haan , Chhayal V. Gandhi , Alan Belcher , Vineet B. Rajgarhia , Allan C. Froehlich , Kristen M. Deleault , Emily Stonehouse , Shital A. Tripathi , Jennifer Gosselin , Yin-Ying Chiu , Haowen Xu
IPC分类号： C12N15/81
CPC分类号： C12N15/81 , C12N9/2405 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12N9/2482 , C12N15/52 , C12P7/06 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01091 , Y02E50/17
摘要： The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.
摘要翻译： 本发明涉及分泌全套或全套的酶的酵母菌株或菌株，以水解玉米淀粉，玉米纤维，木质纤维素（包括水解纤维素中的连接酶，半纤维素和 木质素和碳水化合物之间）并利用戊糖（木糖和阿拉伯糖）。 本发明还涉及在酵母中对于各种酶活性良好表达的蛋白质组。 所得菌株或菌株可以同时水解淀粉和纤维素。 所产生的菌株或菌株也可以代谢工程化以产生较少的甘油和吸收乙酸盐。 所得的菌株或菌株也可用于从颗粒淀粉生产乙醇而不液化。 所得到的菌株或菌株可以进一步用于减少在同时糖化和发酵（SSF）过程中水解生物质原料所需的外部酶的量，或者在当前蔗糖分解酶负载下在SSF期间提高乙醇的产率。 此外，本发明的多种酶可以在本发明的细胞中共表达以提供对生物质原料的协同消化作用。 在一些方面，表达不同异源糖分解酶的宿主细胞也可以共同共培养并用于从生物质原料生产乙醇。
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公开(公告)号：US20090142818A1
公开(公告)日：2009-06-04
申请号：US12300140
申请日：2007-05-11
申请人： Henrik Bisgard-Frantzen , Kevin S. Wenger , Michael Trent Elder , Randy Deinhammer , Joyce Aldridge Craig
发明人： Henrik Bisgard-Frantzen , Kevin S. Wenger , Michael Trent Elder , Randy Deinhammer , Joyce Aldridge Craig
IPC分类号： C12P7/06
CPC分类号： C12P7/06 , C12N9/2417 , C12N9/2428 , C12Y302/01001 , Y02E50/17
摘要： The present invention relates to a process of producing a fermentation product, especially ethanol, from starch-containing material using an alpha-amylase and a carbohydrate-source generating enzyme. The invention also relates to a composition comprising an alpha-amylase and a carbohydrate-source generating enzyme as well as the use such compositions for producing fermentation products.
摘要翻译： 本发明涉及使用α-淀粉酶和碳水化合物源产生酶从含淀粉材料生产发酵产物，特别是乙醇的方法。 本发明还涉及包含α-淀粉酶和碳水化合物源产生酶的组合物，以及用于生产发酵产物的组合物。
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公开(公告)号：US20080227166A1
公开(公告)日：2008-09-18
申请号：US10586097
申请日：2005-01-14
IPC分类号： C12P7/06
CPC分类号： Y02E50/17
摘要： The present invention provides an improved process for producing a fermentation product in a fermentation medium, including ethanol, which process include a fermentation step, comprising subjecting the fermentation medium to at least one surfactant and at least one carbohydrate-source generating enzyme.
摘要翻译： 本发明提供了在发酵培养基（包括乙醇）中生产发酵产物的改进方法，该方法包括发酵步骤，包括使发酵培养基经受至少一种表面活性剂和至少一种产生碳水化合物源的酶。
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公开(公告)号：US07820419B2
公开(公告)日：2010-10-26
申请号：US11814304
申请日：2006-02-07
IPC分类号： C12P7/06
摘要： The present invention relates to a process for producing a fermentation product from starch-containing material, comprising liquefying said starch-containing material with an alpha-amylase; treating with a protease; saccharifying in the presence of a carbohydrate-source generating enzyme; fermenting in the presence of a fermenting organism.
摘要翻译： 本发明涉及一种由含淀粉材料生产发酵产物的方法，包括用α-淀粉酶液化所述含淀粉材料; 用蛋白酶处理; 在碳水化合物源产生酶存在下进行糖化; 在发酵生物存在下发酵。
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公开(公告)号：US20080210541A1
公开(公告)日：2008-09-04
申请号：US11547502
申请日：2005-04-06
IPC分类号： B01D3/34
摘要： The present invention relates to an improved process of distilling fermented mash, wherein one or more amylases and/or proteases are added to the fermentation mash before or during distillation.
摘要翻译： 本发明涉及一种蒸馏发酵的糊状物的改进方法，其中在蒸馏之前或期间将一种或多种淀粉酶和/或蛋白酶加入到发酵醪液中。
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公开(公告)号：US20110183395A1
公开(公告)日：2011-07-28
申请号：US13079100
申请日：2011-04-04
摘要： The present invention relates to processes for producing a fermentation product, such as ethanol, from milled starch-containing material comprising (a) saccharifying the milled starch-containing material with a glucoamylase having an amino acid sequence shown in SEQ ID NO: 2, or a glucoamylase being at least 70% identical thereto, at a temperature below the initial gelatinization temperature of said starch-containing material, (b) fermenting using a fermenting organism.
摘要翻译： 本发明涉及由含碾磨的含淀粉材料生产发酵产物如乙醇的方法，该方法包括：（a）用含有SEQ ID NO：2所示氨基酸序列的葡糖淀粉酶糖化含碾磨淀粉的材料，或 在低于所述含淀粉材料的初始糊化温度的温度下，（b）使用发酵生物发酵的葡糖淀粉酶与其至少70％相同。
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公开(公告)号：US20080138871A1
公开(公告)日：2008-06-12
申请号：US11814304
申请日：2006-02-07
摘要： The present invention relates to a process for producing a fermentation product from starch-containing material, comprising liquefying said starch-containing material with an alpha-amylase; treating with a protease; saccharifying in the presence of a carbohydrate-source generating enzyme; fermenting in the presence of a fermenting organism.
摘要翻译： 本发明涉及一种由含淀粉材料生产发酵产物的方法，包括用α-淀粉酶液化所述含淀粉材料; 用蛋白酶处理; 在碳水化合物源产生酶存在下进行糖化; 在发酵生物存在下发酵。
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