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公开(公告)号：US5180676A
公开(公告)日：1993-01-19
申请号：US668473
申请日：1991-03-13
CPC分类号： C12N5/04 , C12N5/0018 , C12N2500/02 , C12N2501/999
摘要： A method of cultivating animal or plant cells, which consists essentially of contacting a cultivation liquor having animal or plant cells suspended therein with a liquid fluorocarbon having molecular oxygen dissolved therein, characterized in that said cultivation liquor forms a continuous suspension phase which has cell densities of at least 4.times.10.sup.6 cells/ml, that said contact is made by feeding said liquid fluorocarbon into the cultivation liquor from above the cultivation liquor such that 1 ml of liquid fluorocarbon has a surface area of about 6 to about 300 cm.sup.2 whereby the liquid fluorocarbon falls downward through the cultivation liquor by gravity, and that said cultivation liquor has a volume of at least about 10 liters.
摘要翻译： 培养动物或植物细胞的方法，其基本上包括使其中悬浮有动物或植物细胞的培养液与溶解有分子氧的液体碳氟化合物接触，其特征在于所述培养液形成连续悬浮相，其具有细胞密度 至少4×10 6个细胞/ ml，所述接触是通过从培养液上方将所述液体碳氟化合物进料到培养液中制成的，使得1ml液体碳氟化合物的表面积为约6至约300cm 2，由此液体碳氟化合物向下 通过重力通过培养液，并且所述培养液的体积至少约为10升。
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公开(公告)号：US4814278A
公开(公告)日：1989-03-21
申请号：US55332
申请日：1987-05-28
IPC分类号： C12M3/02
摘要： An apparatus for cell culture by perfusion comprising a cell culture tank for suspension culture, said cell culture tank having a suspension cell culture zone, a cell settling zone, an opening for discharging a spent culture medium from the settling zone and an opening for supplying a culture medium to the suspension culture zone, the suspension culture zone and the setting zone being separated by a partition therebetween in a manner to communicate with each other in the lower portion of the settling zone, and the settling zone being formed between the side wall of the cell culture tank and the partition. Another type of apparatus for cell culture having a unit in which main culturing and settling are carried out in a cell settling zone, and a multiunit cell culture apparatus having at least two culturing units which are stacked longitudinally are also described. A method for culturing cells by perfusion in the culture apparatus which comprises withdrawing a substantially cell-free culture medium from the settling zone and introducing a fresh culture medium into the suspension culture zone.
摘要翻译： 一种用于通过灌注进行细胞培养的装置，包括用于悬浮培养的细胞培养槽，所述细胞培养槽具有悬浮细胞培养区，细胞沉降区，用于从沉降区排出废培养基的开口和用于提供 培养基至悬浮培养区，悬浮培养区和固定区由沉积区的下部彼此连通的方式由隔离物隔开，并且沉淀区形成在沉淀区的侧壁之间， 细胞培养槽和分区。 还描述了在细胞沉淀区中进行主要培养和沉降的单元的细胞培养装置的另一种类型，以及具有至少两个纵向堆叠的培养单元的多单元细胞培养装置。 一种培养装置中通过灌注培养细胞的方法，包括从沉降区取出基本上无细胞的培养基并将新鲜培养基引入悬浮培养区。
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公开(公告)号：US5262309A
公开(公告)日：1993-11-16
申请号：US668517
申请日：1991-03-22
申请人： Satoshi Nakamura , Masami Fukuoka , Tsukio Masegi , Kazuo Kitai , Arata Kato , Yataro Ichikawa
发明人： Satoshi Nakamura , Masami Fukuoka , Tsukio Masegi , Kazuo Kitai , Arata Kato , Yataro Ichikawa
CPC分类号： C07K14/525 , A61K38/00
摘要： This invention relates to a novel physiologically active polypeptide containing modified amino acids on both terminals at the amino-terminus and the carboxy-terminus in the amino acid sequence of the human tumor neclosis factor (human TNF), a recombinant plasmid containing a DNA region encoding the polypeptide, a recombinant microorganism cell transformed with the recombinant plasmid, a method of producing the polypeptide by cultivating the microorganism cell, a pharmaceutical composition containing the polypeptide and a method of purifying and recovering the polypeptide. The polypeptide in this invention has excellent antitumor activity compared to the human TNF and can be utilized as an active ingredient of a pharmaceutical composition having antitumor activity.
摘要翻译： PCT No.PCT / JP89 / 00966 Sec。 371日期1991年3月22日 102（e）1991年3月22日PCT申请1989年9月22日PCT公开号 出版物WO90 / 03395 日本1994年4月5日。本发明涉及在人肿瘤坏死因子（人TNF）的氨基酸序列的氨基末端和羧基末端两端含有修饰氨基酸的新的生理活性多肽， 含有编码该多肽的DNA区域的重组质粒，用重组质粒转化的重组微生物细胞，通过培养微生物细胞产生该多肽的方法，含该多肽的药物组合物以及纯化和回收该多肽的方法。 本发明的多肽与人TNF相比具有优异的抗肿瘤活性，可用作具有抗肿瘤活性的药物组合物的活性成分。
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4.发明授权cDNA and genomic DNA encoding the amino acid sequence of human .alpha..sub.2 -plasmin inhibition 失效编码人α2-纤溶酶抑制的氨基酸序列的cDNA和基因组DNA
公开(公告)号：US5221738A
公开(公告)日：1993-06-22
申请号：US803896
申请日：1991-12-09
申请人： Yoshihiko Sumi , Yataro Ichikawa , Masami Murakami , Nobuo Aoki
发明人： Yoshihiko Sumi , Yataro Ichikawa , Masami Murakami , Nobuo Aoki
CPC分类号： C07K14/8121
摘要： cDNA encoding a human .alpha..sub.2 -plasmin inhibitor precursor protein represented by an amino acid sequence from the -39th Met to the 452nd Lys in FIG. 1 of the accompanying drawings; an amino acid sequence of a human .alpha..sub.2 -plasmin inhibitor precursor represented by an amino acid sequence from the -39th Met to the 452nd Lys in FIG. 1 of the accompanying drawings; and genomic DNA encoding a human .alpha..sub.2 -plasmin inhibitor protein which is composed of exons II, III, IV, V, VI, VII, VIII, IX and X in FIG. 4 of the accompanying drawings, said exons being bonded to one another via introns.
摘要翻译： 编码人α2-纤溶酶原抑制物前体蛋白的cDNA，由图3中的第39位Met至第452位Lys的氨基酸序列表示。 附图1; 由图3中的第39位Met至第452位Lys的氨基酸序列表示的人α2-纤溶酶抑制剂前体的氨基酸序列。 附图1; 以及编码图2中由外显子II，III，IV，V，VI，VII，VIII，IX和X组成的人α2-纤溶酶抑制蛋白的基因组DNA。 附图4中，所述外显子通过内含子彼此结合。
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公开(公告)号：US5101024A
公开(公告)日：1992-03-31
申请号：US326417
申请日：1989-03-21
CPC分类号： C12N15/67 , C07K16/00 , C12N15/70 , Y10S435/972 , Y10S530/866 , Y10S530/867
摘要： The present invention provides an enhancer DNA segment, a gene fragment having (a) the enhancer DNA segment and (b) a structural gene such as human D, V and J gene, and a promoter. A hybrid DNA (1) having a phage DNA fragment from Charon 4A phage vector and human DNA fragments, a Charon 4A phage containing the hybrid DNA (1), a hybrid DNA (2) having a PBR322 fragment and human DNA fragment, a transformant of E. coli C600 strain transformed with the hybrid DNA (2), plasmid pSV2-H1G1 obtained by inserting a human DNA fragment into a vector pSV-2gpt, a transformant of E. coli MC 1000 strain transformed with the plasmid pSV2-H1G1 and a transformant of mouse myeloma cells J558L or NS-1 transformed with the plasmid pSV2-H1G1 are also provided.
摘要翻译： 本发明提供增强子DNA片段，具有（a）增强子DNA片段和（b）结构基因如人D，V和J基因的基因片段和启动子。 具有来自Charon 4A噬菌体载体的噬菌体DNA片段和人DNA片段的杂交DNA（1），含有杂合DNA（1）的Charon 4A噬菌体，具有PBR322片段和人DNA片段的杂交DNA（2），转化体 通过将人DNA片段插入载体pSV-2gpt得到的质粒pSV2-H1G1，用质粒pSV2-H1G1转化的大肠杆菌MC1000菌株的转化体，和 还提供了用质粒pSV2-H1G1转化的小鼠骨髓瘤细胞J558L或NS-1的转化体。
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6.发明授权Mouse-human chimaeric immunoglobulin heavy chain specific for the call antigen 失效特异于呼叫抗原的小鼠 - 人类嵌合免疫球蛋白重链
公开(公告)号：US4935496A
公开(公告)日：1990-06-19
申请号：US805381
申请日：1985-12-04
申请人： Akira Kudo , Yushi Nishimura , Yataro Ichikawa , Takeshi Watanabe
发明人： Akira Kudo , Yushi Nishimura , Yataro Ichikawa , Takeshi Watanabe
IPC分类号： C12N15/09 , A61K38/00 , A61K39/395 , C07H21/04 , C07K16/30 , C07K16/46 , C12P21/08 , C12R1/91
CPC分类号： C07K16/462 , C07K16/3061 , A61K38/00 , C07K2317/24 , C07K2319/00 , Y10S530/808 , Y10S530/809 , Y10S530/828 , Y10S530/866 , Y10S530/867
摘要： A mouse-human chimaeric immunoglobulin heavy chain comprising (a) the amino acid sequence of a mouse immunoglobulin heavy chain variable region and (b) the amino acid sequence of a human immunoglobulin heavy chain constant region and reacting specifically with human common acute lympohocytic leukemia antigen and a chimaeric DNA fragment which encodes the amino acid sequence of the above mouse-human chimaeric immunoglobulin heavy chain.
摘要翻译： 一种小鼠 - 人类嵌合免疫球蛋白重链，其包含（a）小鼠免疫球蛋白重链可变区的氨基酸序列和（b）人免疫球蛋白重链恒定区的氨基酸序列，并与人常见的急性淋巴细胞白血病抗原 以及编码上述小鼠 - 人嵌合免疫球蛋白重链的氨基酸序列的嵌合DNA片段。
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公开(公告)号：US4846877A
公开(公告)日：1989-07-11
申请号：US51379
申请日：1987-05-19
摘要： A plant growth promoter comprising as an active ingredient for plant growth promotion at least one phenylglycine selected from the group consisting of(1) monomeric phenylglycines,(2) N-phenylglycylphenylglycines, and(3) cyclic dimers of phenylglycines.
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8.发明授权
公开(公告)号：US4845264A
公开(公告)日：1989-07-04
申请号：US162727
申请日：1988-03-01
IPC分类号： A01N39/00 , A01N39/02 , A01N41/06 , A01N57/18 , C07C65/24 , C07C69/712 , C07C69/738 , C07C69/92 , C07C233/12 , C07C255/53 , C07C311/02
CPC分类号： C07C255/00 , A01N39/02 , A01N41/06 , C07C327/00 , C07C65/24
摘要： A novel phenoxycarboxylic acid of formula (I) is disclosed. The compound is effective as herbicide for eradicating broad-leaved weeds. A combination of the phenoxycarboxylic acid and a N-phosphonomethylglycine or a glufosinate which is known as a herbicide is very effective for eradicating both broad-leaved weeds and narrow-leaved weeds.
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公开(公告)号：US4164518A
公开(公告)日：1979-08-14
申请号：US842312
申请日：1977-10-14
申请人： Yataro Ichikawa , Teizo Yamaji
发明人： Yataro Ichikawa , Teizo Yamaji
CPC分类号： C07C41/01 , C07C2/84 , C07C2531/22
摘要： The oxidative coupling of benzene or benzene derivatives to form the corresponding diphenyls, comprising contacting benzene or a benzene derivative, such as toluene, with molecular oxygen using as a catalyst .beta.-diketo complexes of palladium such as acetylacetone-palladium complex. Use of at least one zirconium compound such as zirconium oxystearate in conjunction with the catalyst gives an increased catalytic activity. The diphenyls are intermediates for production of dyestuffs, pharmaceuticals and other chemicals, and also find application in the preparation of high-molecular-weight polyesters and polyamides.
摘要翻译： 苯或苯衍生物的氧化偶联形成相应的二苯基，包括使用苯或苯衍生物如甲苯与分子氧接触，使用钯如乙酰丙酮 - 钯络合物的β-二酮络合物。 使用至少一种锆化合物如氧化硬脂酸锆与催化剂结合使用提高了催化活性。 二苯基是生产染料，药物和其他化学品的中间体，也可用于制备高分子量聚酯和聚酰胺。
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公开(公告)号：US4151204A
公开(公告)日：1979-04-24
申请号：US771674
申请日：1977-02-24
申请人： Yataro Ichikawa , Eishin Yoshisato , Koji Nakagawa
发明人： Yataro Ichikawa , Eishin Yoshisato , Koji Nakagawa
IPC分类号： C07C215/08 , C07C89/00 , B01J23/40 , C07C85/06
CPC分类号： C07C215/08
摘要： A process for preparing amino alcohols, which comprises reacting a polyhydric alcohol containing at least one primary alcoholic hydroxyl group and at least one secondary alcoholic hydroxyl group and expressed by the following general formula ##STR1## wherein R.sup.1 is an alkyl group, R.sup.2 and R.sup.3 are identical or different and represent a hydrogen atom or a lower alkyl group an n is an integer of 0 to 3,With ammonia in the presence of specified hydrogenation catalyst thereby to aminate the secondary alcoholic hydroxyl group of the polyhydric alcohol selectively.
摘要翻译： 一种制备氨基醇的方法，其包括使含有至少一个伯醇羟基的多元醇与至少一种仲醇羟基反应，并由以下通式表示：其中R 1为烷基，R 2和R 3为 相同或不同，代表氢原子或低级烷基，n为0至3的整数，在规定的氢化催化剂存在下氨基酸可选择性地选择多官能醇的二级醇基羟基。
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