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公开(公告)号：US4814278A
公开(公告)日：1989-03-21
申请号：US55332
申请日：1987-05-28
IPC分类号： C12M3/02
摘要： An apparatus for cell culture by perfusion comprising a cell culture tank for suspension culture, said cell culture tank having a suspension cell culture zone, a cell settling zone, an opening for discharging a spent culture medium from the settling zone and an opening for supplying a culture medium to the suspension culture zone, the suspension culture zone and the setting zone being separated by a partition therebetween in a manner to communicate with each other in the lower portion of the settling zone, and the settling zone being formed between the side wall of the cell culture tank and the partition. Another type of apparatus for cell culture having a unit in which main culturing and settling are carried out in a cell settling zone, and a multiunit cell culture apparatus having at least two culturing units which are stacked longitudinally are also described. A method for culturing cells by perfusion in the culture apparatus which comprises withdrawing a substantially cell-free culture medium from the settling zone and introducing a fresh culture medium into the suspension culture zone.
摘要翻译： 一种用于通过灌注进行细胞培养的装置，包括用于悬浮培养的细胞培养槽，所述细胞培养槽具有悬浮细胞培养区，细胞沉降区，用于从沉降区排出废培养基的开口和用于提供 培养基至悬浮培养区，悬浮培养区和固定区由沉积区的下部彼此连通的方式由隔离物隔开，并且沉淀区形成在沉淀区的侧壁之间， 细胞培养槽和分区。 还描述了在细胞沉淀区中进行主要培养和沉降的单元的细胞培养装置的另一种类型，以及具有至少两个纵向堆叠的培养单元的多单元细胞培养装置。 一种培养装置中通过灌注培养细胞的方法，包括从沉降区取出基本上无细胞的培养基并将新鲜培养基引入悬浮培养区。
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2.发明授权Method of producing useful high-molecular-weight substances by culturing proliferous animal cells, and culture system therefor 失效通过培养增殖动物细胞产生有用的高分子量物质的方法及其培养系统
公开(公告)号：US5447853A
公开(公告)日：1995-09-05
申请号：US870421
申请日：1992-04-17
IPC分类号： C12N5/06 , A61K39/395 , C07K16/00 , C12M3/06 , C12N5/02 , C12N5/10 , C12N5/16 , C12N15/02 , C12P21/08 , C12R1/91
CPC分类号： C12N5/163 , C07K16/00 , C12M29/18 , C12M47/02 , C12M47/04 , C12M47/10 , C12N2500/25 , C12N2500/90
摘要： A method of producing a useful high-molecular-weight substance by culturing animal cells capable of proliferation, which comprises (A) a culturing step of culturing said animal cells in suspension to form a suspension culture mixture containing the animal cells and a useful high-molecular-weight substance produced by the animal cells; (B) a separating step of separating the animal cells from the suspension culture mixture and withdrawing the culture medium; (C) a removing step of removing low-molecular-weight substances inhibiting the growth of the animal cells from the culture medium, said inhibitor substances being the metabolites of the animal cells and different from the useful high-molecular-weight substance; (D) a recycling step of recycling part or the whole of the culture medium left after removal of the growth-inhibiting low-molecular-weight substances to the culturing step (A); and (E) a recovering step of recovering the useful high-molecular-weight substance accumulated in the culture system. A culture system for culturing the animal cells is also described.
摘要翻译： 通过培养能够增殖的动物细胞来生产有用的高分子量物质的方法，其包括（A）在悬浮液中培养所述动物细胞以形成含有动物细胞的悬浮培养混合物的培养步骤， 由动物细胞产生的分子量物质; （B）从悬浮培养混合物中分离动物细胞并取出培养基的分离步骤; （C）从培养基中除去抑制动物细胞生长的低分子量物质的去除步骤，所述抑制剂物质是动物细胞的代谢物，不同于有用的高分子量物质; （D）将除去生长抑制性低分子量物质后剩下的部分或全部培养基再循环至培养步骤（A）的再循环步骤; 和（E）回收在培养体系中积聚的有用的高分子量物质的回收步骤。 还描述了用于培养动物细胞的培养系统。
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公开(公告)号：US5180676A
公开(公告)日：1993-01-19
申请号：US668473
申请日：1991-03-13
CPC分类号： C12N5/04 , C12N5/0018 , C12N2500/02 , C12N2501/999
摘要： A method of cultivating animal or plant cells, which consists essentially of contacting a cultivation liquor having animal or plant cells suspended therein with a liquid fluorocarbon having molecular oxygen dissolved therein, characterized in that said cultivation liquor forms a continuous suspension phase which has cell densities of at least 4.times.10.sup.6 cells/ml, that said contact is made by feeding said liquid fluorocarbon into the cultivation liquor from above the cultivation liquor such that 1 ml of liquid fluorocarbon has a surface area of about 6 to about 300 cm.sup.2 whereby the liquid fluorocarbon falls downward through the cultivation liquor by gravity, and that said cultivation liquor has a volume of at least about 10 liters.
摘要翻译： 培养动物或植物细胞的方法，其基本上包括使其中悬浮有动物或植物细胞的培养液与溶解有分子氧的液体碳氟化合物接触，其特征在于所述培养液形成连续悬浮相，其具有细胞密度 至少4×10 6个细胞/ ml，所述接触是通过从培养液上方将所述液体碳氟化合物进料到培养液中制成的，使得1ml液体碳氟化合物的表面积为约6至约300cm 2，由此液体碳氟化合物向下 通过重力通过培养液，并且所述培养液的体积至少约为10升。
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4.发明授权Method of separating and recovering alkenylbenzenes and unreacted alkylbenzenes from the alkenylation reaction product 失效从烯基化反应产物中分离和回收烯基苯和未反应的烷基苯的方法
公开(公告)号：US3954895A
公开(公告)日：1976-05-04
申请号：US581052
申请日：1975-05-27
申请人： Takeo Shima , Michiyuki Tokashiki , Kazumi Iwata
发明人： Takeo Shima , Michiyuki Tokashiki , Kazumi Iwata
CPC分类号： C07C2/72 , C07C7/04 , C07C2523/04 , C07C2531/12 , Y02P20/582
摘要： In the method wherein an alkenylation reaction product liquid obtained by the alkenylation reaction of an alkylbenzene and a C.sub.4 - C.sub.5 conjugated diene in the presence of alkali metal catalysts and containing unreacted alkylbenzene, alkali metal catalysts and organo-alkalimetal compounds is conveyed to a distillation zone at which said alkenylation reaction product liquid is separated by distillation to recover an alkenylbenzene-containing fraction and an unreacted alkylbenzene-containing fraction, the improvement which comprises adjusting the total of the concentrations of said alkali metal catalysts and organo-alkalimetal compounds in said alkenylation reaction product liquid introduced to the distillation zone so as to be 0.09 - 15 milligram atoms per kilogram of said product liquid, calculated as alkalimetal atoms, and thereafter conducting the distillation of said product liquid under conditions of non-addition of alcohols.
摘要翻译： 在碱金属催化剂和含有未反应的烷基苯的存在下，通过烷基苯和C4-C5共轭二烯的烯基化反应获得的烯化反应产物液体，将碱金属催化剂和有机碱金属化合物输送到蒸馏区 其中通过蒸馏分离所述烯基反应产物液体以回收含烯基苯的馏分和未反应的含烷基的馏分，其改进包括调节所述烯化反应中所述碱金属催化剂和有机碱金属化合物的总浓度 将产物液体引入蒸馏区，每千克所述产物液体为0.09-15毫克原子，以碱金属原子计算，然后在不加入醇的条件下进行所述产物液体的蒸馏。
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公开(公告)号：US3957896A
公开(公告)日：1976-05-18
申请号：US474718
申请日：1974-05-30
IPC分类号： B01J21/00 , B01J23/00 , B01J29/00 , B01J29/22 , C07B61/00 , C07C1/00 , C07C5/22 , C07C5/27 , C07C7/04 , C07C7/14 , C07C15/24 , C07C67/00 , C07C5/24
CPC分类号： C07C15/24 , B01J29/22 , C07C5/2729 , Y10S585/901
摘要： A process for preparing 2,6-dimethylnaphthalene by the selective isomerization of an isomeric mixture of dimethylnaphthalenes(DMN), characterized in that1. the weight ratio of DMN of group A which includes 2,6-DMN, 1,6-DMN, and 1,5-DMN to other DMN (not belonging to group A) in the isomerization reactant is adjusted to not more than 12,2. the weight percent of 2,6-DMN in the reaction product is adjusted to at least 27 based on the DMN of group A contained therein,3. the disproportionation ratio D (weight percent) of other DMN (not belonging to group A) and the conversion G (weight percent) of DMN of group A to other DMN satisfy the relation expressed by D.ltoreq. -0.9G + 6.76, and4. the residue in the crystallization step is recycled to the isomerization step.
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公开(公告)号：US5219752A
公开(公告)日：1993-06-15
申请号：US730386
申请日：1991-07-15
CPC分类号： C12N5/0037 , C12N2500/14 , C12N2500/25
摘要： Adherent 293 human kidney cells are continuously cultured in suspension in serum-free medium. Fresh medium is fed into a culture vessel and spent medium is withdrawn from the vessel. The 293 cells are maintained in small aggregates in suspension at a density of at least 3.times.10.sup.6 cells/ml for 30 days. The formation of large cell clumps is prevented by maintaining the concentration of calcium in the serum-free medium at 0.002 mM to 0.3 mM.
摘要翻译： 将粘附的293人肾细胞在悬浮液中连续培养于无血清培养基中。 将新鲜培养基加入到培养容器中，并从容器中取出废培养基。 将293细胞以至少3×10 6细胞/ ml的密度悬浮保持在悬浮液中的小聚集体30天。 通过将无血清培养基中的钙浓度维持在0.002mM至0.3mM来防止大细胞团的形成。
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7.发明授权
公开(公告)号：US4131594A
公开(公告)日：1978-12-26
申请号：US579064
申请日：1975-05-20
CPC分类号： C08L67/02
摘要： A fire-resistant thermoplastic polyester resin composition having excellent anti-dripping properties which comprises(A) an aromatic polyester resin,(B) a graft copolymer of a butadiene polymer-vinyl monomer in an amount of at least 0.5 parts by weight but less than 25 parts by weight per 100 parts by weight of said polyester resin (A),(c) a fire retardant in an amount of from 0.5 to 35 parts by weight per 100 parts by weight of said polyester resin (A).
摘要翻译： 一种具有优异的抗滴落性能的耐火热塑性聚酯树脂组合物，其包含（A）芳族聚酯树脂，（B）丁二烯聚合物 - 乙烯基单体的接枝共聚物，其量为至少0.5重量份但小于 所述聚酯树脂（A）每100重量份为25重量份，（C）相对于所述聚酯树脂（A）100重量份为0.5〜35重量份的阻燃剂。
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公开(公告)号：US3997616A
公开(公告)日：1976-12-14
申请号：US591247
申请日：1975-06-27
申请人： Michiyuki Tokashiki , Hiroshi Sakai
发明人： Michiyuki Tokashiki , Hiroshi Sakai
CPC分类号： C07C5/31
摘要： A process for the preparation of alkyltetralins which comprises feeding an alkenylbenzene into a reaction zone continuing a fixed bed of an acidic cyclization catalyst, and cyclizing it in the liquid phase at a temperature not higher than about 300.degree. C., characterized in that:I. at least a part of the reaction mixture containing the unreacted alkenylbenzene, which has been withdrawn from said reaction zone and in which the conversion of alkenylbenzene is below the intended final conversion, is cooled, and at least a part of the cooled reaction product in an amount sufficient to adjust the temperature of the reaction zone to not higher than about 300.degree. C. is recycled to said reaction zone, andIi. the remainder of the reaction product is led into another reaction zone containing a fixed bed of acidic cyclization catalyst, and without the aforesaid recycling, further cyclized in the liquid phase until the intended final conversion is attained.
摘要翻译： 一种制备烷基四氢萘酚的方法，其包括将烯基苯进料到连续酸性环化催化剂的固定床的反应区中，并在不高于约300℃的温度下使其在液相中环化，其特征在于：
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9.发明授权
公开(公告)号：US4102853A
公开(公告)日：1978-07-25
申请号：US622498
申请日：1975-10-15
摘要： A fire-retardant thermoplastic polyester resin composition free from "dripping" comprising(A) 100 parts by weight of an aromatic polyester resin,(B) from 0.5 to 100 parts by weight of an alkaline earth metal carbonate, and(C) from 0.5 to 50 parts by weight of a fire retardant.
摘要翻译： 不含“滴”的阻燃热塑性聚酯树脂组合物，其包含（A）100重量份的芳族聚酯树脂，（B）0.5至100重量份的碱土金属碳酸盐和（C）0.5 至50重量份的阻燃剂。
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公开(公告)号：US4018840A
公开(公告)日：1977-04-19
申请号：US536128
申请日：1974-12-24
申请人： Kazumi Iwata , Michiyuki Tokashiki , Takeo Shima
发明人： Kazumi Iwata , Michiyuki Tokashiki , Takeo Shima
CPC分类号： C07C2/72 , C07C2523/04
摘要： In the process for preparing alkenylbenzene by the catalytic reaction of alkylbenzenes and 1,3-butadiene in the presence of an alkali metal catalyst, the improvement which comprises effecting the preliminary contact of the freshly fed alkylbenzene with the reaction product of said catalyst reaction and thereafter catalytically reacting said alkylbenzene with said 1,3-butadiene.
摘要翻译： 在碱金属催化剂存在下，通过烷基苯和1,3-丁二烯的催化反应制备烯基苯的方法，其改进包括进行新鲜进料的烷基苯与所述催化剂反应的反应产物之间的预接触 使所述烷基苯与所述1,3-丁二烯催化反应。
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