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    Nitrile hydratase
    1.
    发明授权
    Nitrile hydratase 失效

    公开(公告)号：US5807730A

    公开(公告)日：1998-09-15

    申请号：US800751

    申请日：1997-02-14

    申请人： Kiyoshi Ito ,  Toshifumi Yamaki ,  Teruo Arii ,  Miyuki Tsuruoka ,  Takeshi Nakamura

    发明人： Kiyoshi Ito ,  Toshifumi Yamaki ,  Teruo Arii ,  Miyuki Tsuruoka ,  Takeshi Nakamura

    IPC分类号： C12N9/78 ,  C12N15/55 ,  C12N9/88

    CPC分类号： C12N9/78 ,  Y10S435/822

    摘要： The present invention provides the amino acid sequence and base sequence of a Pseudonocardia thermophila-derived nitrile hydratase, provides further a method for changing its amino acid sequence and base sequence without substantially changing the functions of said nitrile hydratase, and nitrile hydratases having a base sequence and an amino acid sequence as changed on the basis of said method, and provides furthermore a recombinant plasmid having the gene of said nitrile hydratase, a transformant containing said recombinant plasmid, a method of using said transformant for producing said enzyme, and a method of using said transformant for producing the corresponding amide compound from a nitrile compound.

    Nitrile hydratase
    2.
    发明授权
    Nitrile hydratase 失效
    腈水合酶

    公开(公告)号：US5910432A

    公开(公告)日：1999-06-08

    申请号：US990818

    申请日：1997-12-15

    申请人： Kiyoshi Ito ,  Toshifumi Yamaki ,  Teruo Arii ,  Miyuki Tsuruoka ,  Takeshi Nakamura

    发明人： Kiyoshi Ito ,  Toshifumi Yamaki ,  Teruo Arii ,  Miyuki Tsuruoka ,  Takeshi Nakamura

    IPC分类号： C12N9/78 ,  C12N15/55 ,  C12P13/02 ,  C12N1/00 ,  C12N5/10 ,  C12N9/88 ,  C12N15/60 ,  C12N15/63

    CPC分类号： C12N9/78 ,  Y10S435/822

    摘要： The present invention provides the amino acid sequence and base sequence of a Pseudonocardia thermophila-derived nitrile hydratase, provides further a method for changing its amino acid sequence and base sequence without substantially changing the functions of said nitrile hydratase, and nitrile hydratases having a base sequence and an amino acid sequence as changed on the basis of said method, and provides furthermore a recombinant plasmid having the gene of said nitrile hydratase, a transformant containing said recombinant plasmid, a method of using said transformant for producing said enzyme, and a method of using said transformant for producing the corresponding amide compound from a nitrile compound.

    摘要翻译： 本发明提供了嗜热假诺卡氏菌腈水合酶的氨基酸序列和碱基序列，进一步提供了改变其氨基酸序列和碱基序列而基本上不改变所述腈水合酶的功能的方法，以及具有碱基序列的腈水合酶 和基于所述方法改变的氨基酸序列，并且还提供具有所述腈水合酶基因的重组质粒，含有所述重组质粒的转化体，使用所述转化体产生所述酶的方法，和 使用所述转化体从腈化合物制备相应的酰胺化合物。

    Method For Producing Amide Compound
    5.
    发明申请
    Method For Producing Amide Compound 审中-公开
    生产酰胺化合物的方法

    公开(公告)号：US20090171051A1

    公开(公告)日：2009-07-02

    申请号：US12089511

    申请日：2006-10-06

    申请人： Hiroko Shibamoto ,  Toshikazu Aikawa ,  Teruo Arii ,  Masanori Muramoto ,  Takeshi Fukuda ,  Kiyoshi Ito ,  Takeya Abe ,  Souichi Hazama

    发明人： Hiroko Shibamoto ,  Toshikazu Aikawa ,  Teruo Arii ,  Masanori Muramoto ,  Takeshi Fukuda ,  Kiyoshi Ito ,  Takeya Abe ,  Souichi Hazama

    IPC分类号： C08F22/38 ,  C12P13/02

    CPC分类号： C12P13/00 ,  C07C231/06 ,  C08F220/56 ,  C12P13/02 ,  C07C233/09

    摘要： [Problems] The present invention provides a method for efficiently producing a corresponding amide compound from a nitrile compound by a reaction using a nitrile hydratase and a method for producing an amide-based polymer excellent in quality from the amide compound. In addition, the present invention provides a method for more efficiently producing an acrylamide with higher quality by a microbial catalyst containing a nitrile hydratase and the like and a method for producing an acrylamide-based polymer, which is excellent in hue, has a good balance between the water solubility and the high molecular weight and is also excellent in quality, by using the acrylamide.[Means for Solving the Problems] The method for producing an amide compound of the present invention is characterized in that in a method for producing an amide compound from a nitrile compound in an aqueous medium in the presence of a catalyst having a nitrile hydratase activity, the concentration of benzene in the aqueous medium is 4.0 ppm or less. In addition, the method for producing an amide-based polymer of the present invention is characterized by homopolymerizing the amide compound or by copolymerizing the amide compound and at least unsaturated monomer copolymerizable with the amide compound. Further, the method for producing acrylamide of the present invention is characterized by hydrating acrylonitrile having a concentration of acrolein of 1 ppm or less by a microbial cell containing a nitrile hydratase or a processed product of the microbial cell in an aqueous medium. Furthermore, the method for producing an acrylamide-based polymer of the present invention is characterized by homopolymerizing the acrylamide or by copolymerizing the acrylamide and at least one unsaturated monomer copolymerizable with the acrylamide.

    摘要翻译： 本发明提供一种通过使用腈水合酶的反应从腈化合物有效地制备相应的酰胺化合物的方法和由酰胺化合物质量优异的酰胺类聚合物的制备方法。 此外，本发明提供了一种通过含有腈水合酶等的微生物催化剂更有效地制备丙烯酰胺的方法以及色调优异的丙烯酰胺类聚合物的制造方法，其平衡性良好 在水溶性和高分子量之间，并且通过使用丙烯酰胺也具有优异的质量。 解决问题的手段本发明的酰胺化合物的制造方法的特征在于，在具有腈水合酶活性的催化剂存在下，在含水介质中由腈化合物制备酰胺化合物的方法中， 水介质中苯的浓度为4.0ppm以下。 此外，本发明的酰胺系聚合物的制造方法的特征在于，使酰胺化合物均聚，或者使酰胺化合物与至少与酰胺化合物可共聚的不饱和单体共聚。 此外，本发明的丙烯酰胺的制造方法的特征在于，通过含有腈水合酶的微生物细胞或微生物细胞的加工产物在水性介质中使丙烯醛浓度为1ppm以下的丙烯腈水合。 此外，本发明的丙烯酰胺类聚合物的制造方法的特征在于使丙烯酰胺均聚或通过共聚丙烯酰胺和至少一种与丙烯酰胺共聚的不饱和单体。