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13 条记录 (耗时 0.022 秒)

    Method of producing a galactosyl-cyclodextrin
    1.
    发明授权
    Method of producing a galactosyl-cyclodextrin 失效
    生产半乳糖基 - 环糊精的方法

    公开(公告)号：US5523218A

    公开(公告)日：1996-06-04

    申请号：US368392

    申请日：1994-12-30

    申请人： Sumio Kitahata ,  Koji Hara ,  Koki Fujita ,  Nobuhiro Kuwahara ,  Kyoko Koizumi

    发明人： Sumio Kitahata ,  Koji Hara ,  Koki Fujita ,  Nobuhiro Kuwahara ,  Kyoko Koizumi

    IPC分类号： C08B37/16 ,  C12P19/18 ,  C12P19/04 ,  C12P19/08 ,  C12P19/44

    CPC分类号： A61Q19/00 ,  A61K8/738 ,  C08B37/0012 ,  C12P19/18

    摘要： Disclosed are (1) a novel galactosyl-cyclodextrin having a galactosyl group as bonded to the hydroxyl group of the glucosyl group of a cyclodextrin via an .alpha.-bond and a method of producing said galactosyl-cyclodextrin; (2) a novel galactosyl-cyclodextrin having a galactosyl group as bonded to the hydroxyl group of the glucosyl group of a cyclodextrin via a .beta.-bond and a method of producing said galactosyl-cyclodextrin; and (3) a novel mannosyl-cyclodextrin having a mannosyl group as bonded to the hydroxyl group of the glucosyl group of a cyclodextrin via an .alpha.-bond and a method of producing said mannosyl-cyclodextrin. The new branched cyclodextrins are expected to be widely useful in various fields of drug industry, food industry and cosmetic industry.

    摘要翻译： 公开了（1）具有通过α键与环糊精的葡糖基的羟基键合的半乳糖基的新型半乳糖基 - 环糊精和所述半乳糖基 - 环糊精的制备方法; （2）具有通过β键与环糊精的葡糖基的羟基键合的半乳糖基的新型半乳糖基 - 环糊精和所述半乳糖基 - 环糊精的制造方法; 和（3）具有通过α键与环糊精的葡糖基的羟基键合的甘露糖基的新型甘露糖基 - 环糊精和所述甘露糖基 - 环糊精的制造方法。 新型支链环糊精预期在药物工业，食品工业和化妆品行业的各个领域中广泛应用。

    Reagent for Determining .alpha.-amylase activity and method for determining .alpha.-amylase activity
    6.
    发明授权
    Reagent for Determining .alpha.-amylase activity and method for determining .alpha.-amylase activity 失效
    用于测定α-淀粉酶活性的试剂和测定α-淀粉酶活性的方法

    公开(公告)号：US5393660A

    公开(公告)日：1995-02-28

    申请号：US147717

    申请日：1993-11-04

    申请人： Sumio Kitahata ,  Nobuhiro Kuwahara ,  Koki Fujita ,  Koji Hara ,  Keiichi Majima ,  Shin-ichi Teshima ,  Yuzo Hayashi

    发明人： Sumio Kitahata ,  Nobuhiro Kuwahara ,  Koki Fujita ,  Koji Hara ,  Keiichi Majima ,  Shin-ichi Teshima ,  Yuzo Hayashi

    IPC分类号： C07H15/203 ,  C12Q1/40 ,  C12N9/26

    CPC分类号： C07H15/203 ,  C12Q1/40 ,  C12Q2334/00 ,  C12Q2334/10

    摘要： A reagent for determining a-amylase activity, comprising a maltooligosaccharide derivative of the following formula ##STR1## (wherein either one of R.sub.1 and R.sub.2 is .beta.-galactopyranosil and the other is hydrogen, R.sub.3 is a group bonded to the reducing terminal glucose via a bond cleavable by .alpha.-amylase, which becomes a measurable substance upon cleavage of the bond, and n is an integer of 0-2), which does not comprise adjuvant enzymes; and a method for determining .alpha.-amylase activity which comprises use of the reagent. The reagent of the present invention does not require use of any adjuvant enzyme and is stable since the substrate is not exposed to the decomposition by an adjuvant enzyme. The substrate used in the present invention has high affinity for .alpha.-amylase. Thus, the reagent and the determination method of the present invention make it possible to determine .alpha.-amylase activity with high sensitivity.

    摘要翻译： 一种用于测定α-淀粉酶活性的试剂，包括下式的麦芽低聚糖衍生物（Ⅰ）（其中R 1和R 2中的任一个是β-半乳糖吡喃糖苷，另一个是氢，R 3是键合到还原末端的基团 葡萄糖通过可以被α-淀粉酶切割的键，其在切割键时成为可测量物质，n是0-2的整数），其不包含辅酶; 以及一种测定α-淀粉酶活性的方法，其包括使用该试剂。 本发明的试剂不需要使用任何辅助酶，并且是稳定的，因为底物不被辅助酶暴露于分解。 用于本发明的底物对α-淀粉酶具有高亲和力。 因此，本发明的试剂和测定方法能够以高灵敏度确定α-淀粉酶活性。

    Method for the preparation of fructose-containing oligosaccharide
    9.
    发明授权
    Method for the preparation of fructose-containing oligosaccharide 失效
    含有含葡萄糖的寡糖的制备方法

    公开(公告)号：US5089401A

    公开(公告)日：1992-02-18

    申请号：US672388

    申请日：1991-03-20

    申请人： Koki Fujita ,  Kozo Hara ,  Hitoshi Hashimoto ,  Sumio Kitahata

    发明人： Koki Fujita ,  Kozo Hara ,  Hitoshi Hashimoto ,  Sumio Kitahata

    IPC分类号： C12P19/14 ,  C07H3/06 ,  C12N9/10 ,  C12N9/26 ,  C12P19/12 ,  C12P19/18 ,  C12P19/24 ,  C12R1/06

    CPC分类号： C12N9/2408 ,  C12P19/12 ,  C12P19/24 ,  Y10S435/83

    摘要： An enzymatic method for the preparation of a fructose-containing oligosaccharide, in which a .beta.-fructofuranosidase obtained by culturing Arthrobacter sp. K-1 (FERM BP-3192) as an enzyme is reacted on sucrose, raffinose or stachyose as the donor in the presence of an aldose or ketose as the receptor. The enzyme is characterized by:(1) activity on sucrose for the transglycosidation of a fructosyl group to the receptor in the presence of a monosaccharide, sugar alcohol, alkyl alcohol, glycoside or oligosaccharide;(2) activity for the decomposition of sucrose, elrose, neokestose, xylsucrose, raffinose and stachyose with inactivity on a saccharide selected from the group consisting of 1-kestose, nistose, inulobiose and levan biose;(3) optimum pH of 6.5 to 6.8 at 40.degree. C. with stability at a pH of 5.5 to 10;(4) optimum temperature of 55.degree. C. at a pH of 6.5 exhibiting at least 70% of residual activity at 60.degree. C.;(5) susceptibility to inhibition by the ions of silver, mercury, zinc, copper and tin;(6) two molecular weights of 52,000.+-.2,500 and 58,000.+-.2,500; and(7) two isoelectric points of pH 4.3 and pH 4.6.