公开(公告)号：US20190091648A1

公开(公告)日：2019-03-28

申请号：US16206938

申请日：2018-11-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander MASTROIANNI ,  Scott C. BENSON ,  Steven M. MENCHEN ,  John SCOTT

IPC: B01J19/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869

Abstract: A method of preparing a discrete polymer network array include mixing a plurality of nucleic acid polymer networks with a plurality of color-activated polymer networks to form a dispersion, applying the dispersion to an array of wells, the nucleic acid polymer networks selectively depositing into wells of the array of wells, and rinsing the array of wells to selectively remove the plurality of color-activated polymer networks.

公开(公告)号：US20240110063A1

公开(公告)日：2024-04-04

申请号：US18345621

申请日：2023-06-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Andrew LEVITZ ,  Khairuzzaman Bashar MULLAH ,  Brian EVANS ,  Scott C. BENSON ,  Chris VONNEGUT

IPC: C09B57/14 ,  C12Q1/6818 ,  C12Q1/6844 ,  G01N29/24

CPC classification number: C09B57/14 ,  C12Q1/6818 ,  C12Q1/6844 ,  G01N29/2418

Abstract: The present disclosure relates to dibenzoxanthene compounds that are efficient quenchers of fluorescence, for example in the far red and near infrared spectrum. Applications using the dibenzoxanthene quenching compounds and methods of making same are also described.

公开(公告)号：US20170007977A1

公开(公告)日：2017-01-12

申请号：US15203582

申请日：2016-07-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Alexander MASTROIANNI ,  Scott C. BENSON ,  Steven M. MENCHEN ,  John SCOTT

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q2535/122 ,  C12Q2563/155 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: A method of preparing a discrete polymer network array include mixing a plurality of nucleic acid polymer networks with a plurality of color-activated polymer networks to form a dispersion, applying the dispersion to an array of wells, the nucleic acid polymer networks selectively depositing into wells of the array of wells, and rinsing the array of wells to selectively remove the plurality of color-activated polymer networks.

Abstract translation: 制备离散聚合物网络阵列的方法包括将多个核酸聚合物网络与多个着色活化聚合物网络混合以形成分散体，将该分散体施加到孔阵列上，将核酸聚合物网络选择性地沉积到孔中 的孔阵列，并且冲洗孔阵列以选择性地去除多个着色活化聚合物网络。

公开(公告)号：US20150206762A1

公开(公告)日：2015-07-23

申请号：US14676697

申请日：2015-04-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Christina E. INMAN ,  Alexander MASTROIANNI ,  Wolfgang HINZ ,  Shifeng LI ,  Scott C. BENSON

IPC: H01L21/311 ,  G01N27/414

CPC classification number: H01L21/31105 ,  G01N27/4145 ,  H01L21/31144

Abstract: A method of manufacturing a sensor, the method including forming an array of chemically-sensitive field effect transistors (chemFETs), depositing a dielectric layer over the chemFETs in the array, depositing a protective layer over the dielectric layer, etching the dielectric layer and the protective layer to form cavities corresponding to sensing surfaces of the chemFETs, and removing the protective layer. The method further includes, etching the dielectric layer and the protective layer together to form cavities corresponding to sensing surfaces of the chemFETs. The protective layer is at least one of a polymer, photoresist material, noble metal, copper oxide, and zinc oxide. The protective protective layer is removed using at least one of sodium hydroxide, organic solvent, aqua regia, ammonium carbonate, hydrochloric acid, acetic acid, and phosphoric acid.

Abstract translation: 一种制造传感器的方法，所述方法包括形成化学敏感场效应晶体管阵列（chemFET），在阵列中的chemFET上沉积电介质层，在电介质层上沉积保护层，蚀刻电介质层和 保护层以形成对应于chemFET的感测表面的空腔，以及去除保护层。 该方法还包括：将电介质层和保护层一起蚀刻以形成对应于chemFET的感测表面的空腔。 保护层是聚合物，光致抗蚀剂材料，贵金属，氧化铜和氧化锌中的至少一种。 使用氢氧化钠，有机溶剂，王水，碳酸铵，盐酸，乙酸和磷酸中的至少一种除去保护性保护层。

公开(公告)号：US20230124451A1

公开(公告)日：2023-04-20

申请号：US18076986

申请日：2022-12-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Khairuzzaman Bashar MULLAH ,  Brian EVANS ,  Scott C. BENSON ,  Chu-An CHANG ,  Xiongwei YAN

IPC: C12Q1/6818 ,  C12Q1/686

Abstract: Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.