公开(公告)号：US20210324460A1

公开(公告)日：2021-10-21

申请号：US17305239

申请日：2021-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shiaw-Min CHEN ,  Elana SWARTZMAN ,  David RUFF ,  Mark SHANNON ,  Julia LU ,  Stephen HENDRICKS

IPC: C12Q1/6855 ,  C12Q1/6804

Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

公开(公告)号：US20200048695A1

公开(公告)日：2020-02-13

申请号：US16656839

申请日：2019-10-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shiaw-Min CHEN ,  Elana SWARTZMAN ,  David RUFF ,  Mark SHANNON ,  Julia LU ,  Stephen HENDRICKS

IPC: C12Q1/6855 ,  C12Q1/6804

Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

公开(公告)号：US20170045428A1

公开(公告)日：2017-02-16

申请号：US15233801

申请日：2016-08-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mousumi RATH ,  Mark SHANNON ,  Jason LA

IPC: G01N1/28 ,  C12Q1/68

CPC classification number: G01N1/04 ,  B01L3/50825 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/0838 ,  C12Q1/6806 ,  G01N1/2813 ,  G01N1/286 ,  G01N1/34 ,  G01N2001/028 ,  G01N2001/045 ,  G01N2001/284 ,  G01N2001/2886 ,  C12Q2521/327 ,  C12Q2527/101 ,  C12Q2527/113 ,  C12Q2531/113 ,  C12Q2565/601 ,  C12Q2535/122

Abstract: In one embodiment, a method for processing a sample includes selecting a selected sample from a biological specimen, the selected sample being in contact with a first surface of a first substrate. The method also includes transferring the selected sample directly from the first surface to a container comprising an internal volume. The method also includes forming or providing a sample solution within the internal volume of the container by contacting the selected sample with a lysis mixture using a protocol. The method further includes performing an assay, experiment, or test on the sample solution while the sample solution disposed is within the internal volume of the container.In another embodiment, a method for processing a sample includes providing a selected sample comprising one or more cells. The method also includes transferring the selected sample into an internal volume of a container. The method also includes contacting the selected sample with a lysis mixture using a protocol to provide a sample solution, wherein the protocol comprises heating the sample solution to a first temperature that is greater than 37 degrees Celsius and less than or equal to 75 degrees Celsius.

Abstract translation: 在另一个实施方案中，用于处理样品的方法包括提供包含一个或多个细胞的选定样品。 该方法还包括将所选择的样品转移到容器的内部容积中。 该方法还包括使用方案使所选样品与裂解混合物接触以提供样品溶液，其中所述方案包括将样品溶液加热至大于37摄氏度且小于或等于75摄氏度的第一温度。