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    METHODS FOR DYE SELECTION FOR PROTEIN MELT TEMPERATURE DETERMINATIONS
    1.
    发明申请
    METHODS FOR DYE SELECTION FOR PROTEIN MELT TEMPERATURE DETERMINATIONS 有权

    公开(公告)号：US20210063405A1

    公开(公告)日：2021-03-04

    申请号：US17016178

    申请日：2020-09-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Carole BORNARTH ,  Mousumi RATH

    IPC: G01N33/58 ,  G01N33/68 ,  C09B23/10 ,  C09B23/14

    Abstract: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.

    BIOLOGICAL SAMPLE PREPARATION FOR TESTING
    5.
    发明申请
    BIOLOGICAL SAMPLE PREPARATION FOR TESTING 审中-公开
    生物样品制备测试

    公开(公告)号：US20170045428A1

    公开(公告)日：2017-02-16

    申请号：US15233801

    申请日：2016-08-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mousumi RATH ,  Mark SHANNON ,  Jason LA

    IPC: G01N1/28 ,  C12Q1/68

    CPC classification number: G01N1/04 ,  B01L3/50825 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/0838 ,  C12Q1/6806 ,  G01N1/2813 ,  G01N1/286 ,  G01N1/34 ,  G01N2001/028 ,  G01N2001/045 ,  G01N2001/284 ,  G01N2001/2886 ,  C12Q2521/327 ,  C12Q2527/101 ,  C12Q2527/113 ,  C12Q2531/113 ,  C12Q2565/601 ,  C12Q2535/122

    Abstract: In one embodiment, a method for processing a sample includes selecting a selected sample from a biological specimen, the selected sample being in contact with a first surface of a first substrate. The method also includes transferring the selected sample directly from the first surface to a container comprising an internal volume. The method also includes forming or providing a sample solution within the internal volume of the container by contacting the selected sample with a lysis mixture using a protocol. The method further includes performing an assay, experiment, or test on the sample solution while the sample solution disposed is within the internal volume of the container.In another embodiment, a method for processing a sample includes providing a selected sample comprising one or more cells. The method also includes transferring the selected sample into an internal volume of a container. The method also includes contacting the selected sample with a lysis mixture using a protocol to provide a sample solution, wherein the protocol comprises heating the sample solution to a first temperature that is greater than 37 degrees Celsius and less than or equal to 75 degrees Celsius.

    Abstract translation: 在另一个实施方案中，用于处理样品的方法包括提供包含一个或多个细胞的选定样品。 该方法还包括将所选择的样品转移到容器的内部容积中。 该方法还包括使用方案使所选样品与裂解混合物接触以提供样品溶液，其中所述方案包括将样品溶液加热至大于37摄氏度且小于或等于75摄氏度的第一温度。

    METHODS FOR DYE SELECTION FOR PROTEIN MELT TEMPERATURE DETERMINATIONS
    6.
    发明申请
    METHODS FOR DYE SELECTION FOR PROTEIN MELT TEMPERATURE DETERMINATIONS 有权
    用于蛋白质熔融温度测定的染色体选择方法

    公开(公告)号：US20140315190A1

    公开(公告)日：2014-10-23

    申请号：US14213204

    申请日：2014-03-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Carole BORNARTH ,  Mousumi RATH

    IPC: G01N33/557 ,  G01N33/58

    CPC classification number: G01N33/582 ,  C09B23/10 ,  C09B23/105 ,  C09B23/107 ,  C09B23/145 ,  G01N33/6803

    Abstract: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.

    Abstract translation: 根据本教导，提供了利用一种或多种荧光团染料的蛋白质熔融分析的组合物，试剂盒和方法。 在一些实施方案中，方法包括通过将至少一种蛋白质与两种或更多种染料混合并在记录样品的荧光发射的同时施加受控加热来制备样品。 该方法可用于例如筛选优化蛋白质稳定性的条件，筛选结合并增强蛋白质稳定性的配体（例如，蛋白质 - 蛋白质相互作用），筛选突变以提高稳定性，筛选结晶条件以获得蛋白质稳定性，筛选 用于蛋白质稳定性的储存条件，以及蛋白质稳定性使用蛋白质的筛选条件（例如，生产条件，处理条件等）。

    DEVICES AND METHODS FOR LASER CAPTURE MICRODISSECTION
    7.
    发明申请
    DEVICES AND METHODS FOR LASER CAPTURE MICRODISSECTION 有权

    公开(公告)号：US20210148792A1

    公开(公告)日：2021-05-20

    申请号：US17162034

    申请日：2021-01-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Joel COLBURN ,  Mousumi RATH ,  Sarah GHANBARI

    IPC: G01N1/04 ,  C12Q1/6806 ,  G01N1/28

    Abstract: A carrier for holding a biological sample includes a substrate. The substrate is configured to engage a first sample chamber comprising a first opening characterized by a first opening diameter or a second sample chamber comprising a second opening characterized by a second opening diameter that is greater than the first opening diameter. The substrate includes an upper portion, a lower portion, and an intermediate portion disposed between the upper portion and the lower portion. The lower portion is disposed below the upper portion and comprises a bottom surface configured to receive a biological sample. The intermediate portion is characterized by a first substrate diameter and the lower portion is characterized by a second substrate diameter that is less than the first substrate diameter.

    METHODS FOR DYE SELECTION FOR PROTEIN MELT TEMPERATURE DETERMINATIONS
    8.
    发明申请
    METHODS FOR DYE SELECTION FOR PROTEIN MELT TEMPERATURE DETERMINATIONS 审中-公开

    公开(公告)号：US20180348230A1

    公开(公告)日：2018-12-06

    申请号：US16001613

    申请日：2018-06-06

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Carole BORNARTH ,  Mousumi RATH

    IPC: G01N33/58 ,  C09B23/14 ,  C09B23/10 ,  G01N33/68

    CPC classification number: G01N33/582 ,  C09B23/10 ,  C09B23/105 ,  C09B23/107 ,  C09B23/145 ,  G01N33/6803

    Abstract: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.

    DEVICES AND METHODS FOR LASER CAPTURE MICRODISSECTION
    9.
    发明申请
    DEVICES AND METHODS FOR LASER CAPTURE MICRODISSECTION 审中-公开
    用于激光捕获显微镜的装置和方法

    公开(公告)号：US20170045421A1

    公开(公告)日：2017-02-16

    申请号：US15233756

    申请日：2016-08-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Joel COLBURN ,  Mousumi RATH ,  Sarah GHANBARI

    IPC: G01N1/04 ,  G01N1/28

    CPC classification number: G01N1/04 ,  B01L3/50825 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/0838 ,  C12Q1/6806 ,  G01N1/2813 ,  G01N1/286 ,  G01N1/34 ,  G01N2001/028 ,  G01N2001/045 ,  G01N2001/284 ,  G01N2001/2886 ,  C12Q2521/327 ,  C12Q2527/101 ,  C12Q2527/113 ,  C12Q2531/113 ,  C12Q2565/601 ,  C12Q2535/122

    Abstract: A carrier for holding a biological sample includes a substrate. The substrate is configured to engage a first sample chamber comprising a first opening characterized by a first opening diameter or a second sample chamber comprising a second opening characterized by a second opening diameter that is greater than the first opening diameter. The substrate includes an upper portion, a lower portion, and an intermediate portion disposed between the upper portion and the lower portion. The lower portion is disposed below the upper portion and comprises a bottom surface configured to receive a biological sample. The intermediate portion is characterized by a first substrate diameter and the lower portion is characterized by a second substrate diameter that is less than the first substrate diameter.

    Abstract translation: 用于保持生物样品的载体包括基底。 衬底被构造成接合第一样品室，所述第一样品室包括由第一开口直径或第二样品室表征的第一开口，所述第一开口包括第二开口，第二开口的特征在于第二开口直径大于第一开口直径。 基板包括上部，下部和设置在上部和下部之间的中间部。 下部设置在上部下方，并且包括构造成接纳生物样品的底面。 中间部分的特征在于第一基底直径，下部的特征在于第二基底直径小于第一基底直径。

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