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    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    1.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 有权

    公开(公告)号：US20210032679A1

    公开(公告)日：2021-02-04

    申请号：US16949159

    申请日：2020-10-15

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John LEAMON ,  Mark ANDERSEN ,  Michael THORNTON

    IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12N15/10 ,  C12Q1/6886 ,  C12Q1/6874

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

    MULTIPLEX TRANSCRIPTOME ANALYSIS
    2.
    发明申请
    MULTIPLEX TRANSCRIPTOME ANALYSIS 审中-公开
    多重代码分析

    公开(公告)号：US20150344938A1

    公开(公告)日：2015-12-03

    申请号：US14826385

    申请日：2015-08-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Kelli BRAMLETT ,  John LEAMON ,  Mark ANDERSEN ,  Michael THORNTON

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2525/117

    Abstract: In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing RNA or DNA, including whole transcriptome or genomic samples. In some embodiments, the sequences and abundances of at least some of the plurality of amplicons are characterized, optionally simultaneously or through a single assay, by suitable detection methods, including sequencing or other procedures known in the art.

    Abstract translation: 在一些实施方案中，本公开一般涉及包含多重核酸扩增反应的方法，组合物，系统，装置和试剂盒，所述多重核酸扩增反应采用多个（例如，数百，数万，数万或数十万）不同的 靶特异性引物对，其能够在单个反应混合物中基本上同时扩增感兴趣的多个不同靶序列。 在一些实施方案中，多重核酸扩增反应产生具有衍生自含有RNA或DNA的样品的序列的多个扩增子，包括全转录组或基因组样品。 在一些实施方案中，通过合适的检测方法，包括测序或本领域已知的其它程序，可以任选地同时或通过单个测定来表征多个扩增子中的至少一些的序列和丰度。

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    5.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开
    多重PCR的方法和组合

    公开(公告)号：US20130059738A1

    公开(公告)日：2013-03-07

    申请号：US13663334

    申请日：2012-10-29

    Applicant: Life Technologies Corporation

    Inventor： John LEAMON ,  Mark ANDERSEN ,  Michael THORNTON

    IPC: C40B20/00

    CPC classification number: C12Q1/686 ,  C12Q1/6855 ,  C12Q1/6879 ,  C12Q1/6883 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2537/16

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for determining copy number variation of one or more nucleic acids present in a sample. In some aspects, the method includes various target-specific primers that allow for the selective amplification of one or more target nucleic acids in the sample. In yet another aspect, the invention relates to determining copy number variation with respect to gene or chromosome representation of a nucleic acid in the sample. In some aspects, the method for determining copy number variation of different target nucleic acids in a sample using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including diagnosis, predictive therapeutic regimes or other therapeutic purposes.

    Abstract translation: 本发明提供了可用于确定样品中存在的一种或多种核酸的拷贝数变异的方法，组合物，试剂盒，系统和装置。 在一些方面，该方法包括允许选择性扩增样品中一种或多种靶核酸的各种靶特异性引物。 在另一方面，本发明涉及确定相对于样品中核酸的基因或染色体表示的拷贝数变异。 在一些方面，使用所公开的方法，试剂盒，系统和装置确定样品中不同靶核酸的拷贝数变异的方法可以用于包括诊断，预测性治疗方案或其他治疗目的的各种下游过程。

    MULTIPLEX TRANSCRIPTOME ANALYSIS
    8.
    发明申请
    MULTIPLEX TRANSCRIPTOME ANALYSIS 审中-公开

    公开(公告)号：US20160194694A1

    公开(公告)日：2016-07-07

    申请号：US15068466

    申请日：2016-03-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Kelli BRAMLETT ,  John LEAMON ,  Mark ANDERSEN ,  Michael THORNTON

    IPC: C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2525/117

    Abstract: In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing RNA or DNA, including whole transcriptome or genomic samples. In some embodiments, the sequences and abundances of at least some of the plurality of amplicons are characterized, optionally simultaneously or through a single assay, by suitable detection methods, including sequencing or other procedures known in the art.

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    9.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开

    公开(公告)号：US20130059762A1

    公开(公告)日：2013-03-07

    申请号：US13679706

    申请日：2012-11-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John LEAMON ,  Mark ANDERSEN ,  Michael THORNTON

    IPC: C40B50/06

    CPC classification number: C12Q1/6855 ,  C12Q1/6806 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

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