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公开(公告)号：US08642337B2
公开(公告)日：2014-02-04
申请号：US13291555
申请日：2011-11-08
CPC分类号： C12N5/0068 , C12N2503/02 , C12N2535/10 , G01N33/569 , G01N2610/00
摘要： A method for the construction of arrays from self-assembling monolayers is described. The arrays have particular utility for the screening of peptides ligands that can foster the growth of cells in culture. This technique has been used to identify peptide ligands that foster the growth of human stem cells, which otherwise require an extracellular matrix in order to grow in an undifferentiated state. This also makes possible an assay to identify other such peptides.
摘要翻译： 描述了用于从自组装单层构建阵列的方法。 阵列对于能够促进培养细胞生长的肽配体的筛选具有特别的用途。 已经使用这种技术鉴定促进人类干细胞生长的肽配体，否则需要细胞外基质以在未分化状态下生长。 这也使鉴定其他这样的肽成为可能。
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公开(公告)号：US08062890B2
公开(公告)日：2011-11-22
申请号：US11504573
申请日：2006-08-15
CPC分类号： C12N5/0068 , C12N2503/02 , C12N2535/10 , G01N33/569 , G01N2610/00
摘要： A method for the construction of arrays from self-assembling monolayers is described. The arrays have particular utility for the screening of peptides ligands that can foster the growth of cells in culture. This technique has been used to identify peptide ligands that foster the growth of human stem cells, which otherwise require an extracellular matrix in order to grow in an undifferentiated state. This also makes possible an assay to identify other such peptides.
摘要翻译： 描述了用于从自组装单层构建阵列的方法。 阵列对于能够促进培养细胞生长的肽配体的筛选具有特别的用途。 已经使用这种技术鉴定促进人类干细胞生长的肽配体，否则需要细胞外基质以在未分化状态下生长。 这也使鉴定其他这样的肽成为可能。
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公开(公告)号：US20120135518A1
公开(公告)日：2012-05-31
申请号：US13291555
申请日：2011-11-08
IPC分类号： C12N5/071
CPC分类号： C12N5/0068 , C12N2503/02 , C12N2535/10 , G01N33/569 , G01N2610/00
摘要： A method for the construction of arrays from self-assembling monolayers is described. The arrays have particular utility for the screening of peptides ligands that can foster the growth of cells in culture. This technique has been used to identify peptide ligands that foster the growth of human stem cells, which otherwise require an extracellular matrix in order to grow in an undifferentiated state. This also makes possible an assay to identify other such peptides.
摘要翻译： 描述了用于从自组装单层构建阵列的方法。 阵列对于能够促进培养细胞生长的肽配体的筛选具有特别的用途。 已经使用这种技术鉴定促进人类干细胞生长的肽配体，否则需要细胞外基质以在未分化状态下生长。 这也使鉴定其他这样的肽成为可能。
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公开(公告)号：US09133266B2
公开(公告)日：2015-09-15
申请号：US13464235
申请日：2012-05-04
申请人： James A. Thomson , Zhonggang Hou , Guokai Chen
发明人： James A. Thomson , Zhonggang Hou , Guokai Chen
IPC分类号： C12N5/071 , C07K14/78 , C12N5/0735 , C12N5/074
CPC分类号： C12N5/0606 , C07K14/78 , C12N5/0696 , C12N2500/02 , C12N2500/05 , C12N2500/25 , C12N2500/38 , C12N2501/115 , C12N2501/15 , C12N2501/33 , C12N2501/727 , C12N2533/50 , C12N2533/52
摘要： Vitronectin-derived cell culture substrates and methods of using the same for culturing pluripotent stem cells are presented.
摘要翻译： 提供了来自Vitronectin的细胞培养物底物及其使用培养多能干细胞的方法。
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公开(公告)号：US20120202291A1
公开(公告)日：2012-08-09
申请号：US13204354
申请日：2011-08-05
申请人： Guokai Chen , James A. Thomson
发明人： Guokai Chen , James A. Thomson
CPC分类号： C12N5/0606 , C12N5/0696 , C12N2500/25 , C12N2500/38 , C12N2500/92 , C12N2500/98 , C12N2501/115 , C12N2501/15 , C12N2501/39 , C12N2501/727 , C12N2501/999
摘要： Fully defined media that support pluripotent cell viability, proliferation, cloning, and derivation, as well as methods and compositions including these media are described. Methods for deriving iPS cells from adult individuals under defined, xeno-free conditions are also described.
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公开(公告)号：US20120142106A1
公开(公告)日：2012-06-07
申请号：US13364074
申请日：2012-02-01
IPC分类号： C12N5/0784 , C12N15/07
CPC分类号： C12N5/0639 , C12N5/064 , C12N2506/02
摘要： This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.
摘要翻译： 本发明涉及来自人胚胎干（ES）细胞的树突状细胞的培养。 首先通过与基质细胞共培养将人类ES细胞培养成造血细胞。 然后将分化成造血谱系的细胞与GM-CSF一起培养以产生骨髓前体细胞的培养物。 用细胞因子GM-CSF和IL-4培养骨髓前体细胞会导致产生功能性树突状细胞。 树突细胞具有独特的表型，如细胞表面标志物的组合所示。
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公开(公告)号：US07892830B2
公开(公告)日：2011-02-22
申请号：US12016066
申请日：2008-01-17
申请人： Veit Bergendahl , James A. Thomson
发明人： Veit Bergendahl , James A. Thomson
CPC分类号： C12N5/0606 , C12N2501/70 , C12N2501/999
摘要： While culture medium and systems have been described that permit the culture and proliferation of human embryonic stem cells in feeder free and animal product free conditions, these conditions will not readily support cloning of an embryonic stem cell culture meaning, at least here, the initiation of a sub-culture using one or a very few originating cells. It has been found here that a class of small molecules that are inhibitors of kinase enzymes will increase the efficiency of cloning of stem cell cultures sufficiently to make such cloning practical in the defined medium and in other media as well.
摘要翻译： 虽然已经描述了允许人类胚胎干细胞在无饲养细胞和无动物产品条件下的培养和增殖的培养基和系统，但是这些条件将不容易支持克隆胚胎干细胞培养物，至少在这里，启动 一种使用一种或几种原始细胞的亚培养物。 这里已经发现，作为激酶的抑制剂的一类小分子将提高干细胞培养物的克隆效率，使得这种克隆在定义的培养基和其它培养基中也是实用的。
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8.发明申请GENERATION OF CLONAL MESENCHYMAL PROGENITORS AND MESENCHYMAL STEM CELL LINES UNDER SERUM-FREE CONDITIONS 有权在无血清条件下产生骨髓间充质干细胞和细胞干细胞细胞系公开(公告)号：US20090081784A1
公开(公告)日：2009-03-26
申请号：US12024770
申请日：2008-02-01
IPC分类号： C12N5/06
CPC分类号： C12N5/0662 , C12N2500/90 , C12N2501/115 , C12N2502/1394 , C12N2506/02 , C12N2510/00 , C12N2799/027
摘要： Methods for obtaining multipotent mesenchymal stem cells under serum-free conditions and methods for identifying multipotent mesenchymal progenitor cells are disclosed.
摘要翻译： 公开了在无血清条件下获得多能间充质干细胞的方法和用于鉴定多能间充质祖细胞的方法。
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公开(公告)号：US20090029464A1
公开(公告)日：2009-01-29
申请号：US12240657
申请日：2008-09-29
申请人： Ren-He Xu , James A. Thomson
发明人： Ren-He Xu , James A. Thomson
CPC分类号： C12N5/0606 , C12N2501/115 , C12N2501/155
摘要： Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if an antagonist of bone morphogenic protein is added to the medium in which the stem cells are cultured, together with fibroblast growth factor, the stem cells will remain undifferentiated indefinitely, even without feeder cells or conditioned medium.
摘要翻译： 用于培养人胚胎干细胞的以前的方法需要成纤维细胞饲养细胞或已经暴露于成纤维细胞饲养细胞的培养基，以便保持干细胞处于未分化状态。 现在已经发现，如果将骨形态发生蛋白的拮抗剂加入到其中培养干细胞的培养基中，与成纤维细胞生长因子一起，干细胞将无限期地保持未分化，即使没有饲养细胞或条件培养基。
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公开(公告)号：US20090023208A1
公开(公告)日：2009-01-22
申请号：US12240640
申请日：2008-09-29
申请人： James A. Thomson , Mark Levenstein
发明人： James A. Thomson , Mark Levenstein
IPC分类号： C12N5/06
CPC分类号： C12N5/0606 , C12N2500/25 , C12N2500/44 , C12N2500/90 , C12N2501/115
摘要： The invention relates to methods for culturing human embryonic stem cells by culturing the stem cells in an environment essentially free of mammalian fetal serum and in a stem cell culture medium including amino acids, vitamins, salts, minerals, transferring, insulin, albumin, and a fibroblast growth factor that is supplied from a source other than just a feeder layer the medium. Also disclosed are compositions capable of supporting the culture and proliferation of human embryonic stem cells without the need for feeder cells or for exposure of the medium to feeder cells.
摘要翻译： 本发明涉及通过在基本上不含哺乳动物胎儿血清的环境中和在包括氨基酸，维生素，盐，矿物质，转移，胰岛素，白蛋白和/或细胞的干细胞培养基中培养干细胞来培养人胚胎干细胞的方法。 成纤维细胞生长因子从源而不仅仅是饲养层提供给培养基。 还公开了能够支持人胚胎干细胞的培养和增殖而不需要饲养细胞或将培养基暴露于饲养细胞的组合物。
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