公开(公告)号：US5641404A

公开(公告)日：1997-06-24

申请号：US596338

申请日：1996-02-20

申请人： Lawrence W. Nicholson ,  Christian T. Goralski ,  Curtis D. Pfeiffer

发明人： Lawrence W. Nicholson ,  Christian T. Goralski ,  Curtis D. Pfeiffer

IPC分类号： B01D15/08 ,  C07B57/00

CPC分类号： B01D15/3833 ,  B01D15/203 ,  C07B57/00

摘要： A process for separating enantiomeric mixtures by liquid chromatography using a stationary phase that includes cellulose or amylose derivative and a mobile phase that includes methanol and pentane, the concentration of methanol in the mobile phase being greater than one tenth percent by volume and less than the saturation concentration of methanol in the mobile phase, the concentration of pentane being at least that necessary to resolve the enantiomeric mixture into its enantiomers with a resolution at least one and one half times greater than the pentane of the mobile phase is replaced with hexane.

摘要翻译： PCT No.PCT / US94 / 09687 371日期1996年2月20日 102（e）日期1996年2月20日PCT 1994年8月25日PCT公布。 第WO95 / 05879号公报 日期1995年3月2日使用包含纤维素或直链淀粉衍生物的固定相和包含甲醇和戊烷的流动相通过液相色谱分离对映异构体混合物的方法，流动相中甲醇的浓度大于体积百分之十 并且小于流动相中甲醇的饱和浓度，所替代的戊烷浓度至少为将对映异构体混合物分解成其对映异构体所必需的浓度至少比流动相的戊烷高1.5倍。 用己烷。

公开(公告)号：US06790669B1

公开(公告)日：2004-09-14

申请号：US10018629

申请日：2001-12-14

申请人： Curtis D. Pfeiffer ,  Nile N. Frawley ,  Thomas L. Peters ,  Philip J. Savickas ,  David R. Albers ,  Steven J. Gluck ,  Lawrence W. Nicholson ,  Jose B. Esquivel H.

发明人： Curtis D. Pfeiffer ,  Nile N. Frawley ,  Thomas L. Peters ,  Philip J. Savickas ,  David R. Albers ,  Steven J. Gluck ,  Lawrence W. Nicholson ,  Jose B. Esquivel H.

IPC分类号： G01N3014

CPC分类号： G01N30/88 ,  G01N30/78 ,  G01N2030/625 ,  G01N2030/8813 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/255

摘要： A chemical analysis method for determining chemically related differences between subject biological material such as genetically modified plant material and control biological material such as genetically unmodified plant material, which method includes at least the following six steps. The first step is to contact the subject biological material with a fluid extractant, such as a mixture of water, isopropanol and potassium hydroxide, to produce a fluid extract of the subject biological material. The second step is to contact the control biological material with the fluid extractant to produce a fluid extract of the control biological material. The third step is to chromatograph the fluid extract of the subject biological material, for example, gas or fluid chromatography, to produce a chromatogram of the fluid extract of the subject biological material. The fourth step is to chromatograph the fluid extract of the control biological material to produce a chromatogram of the fluid extract of the control biological material. The fifth step is to determine the differences between the chromatograms, for example, by using the method of U.S. Pat. No. 5,592,402, to identify at least one outlier peak. The sixth step is to determine the chemical identity of the outlier peak, for example, using gas chromatography/mass spectroscopy analysis of the outlier peak.

摘要翻译： 一种化学分析方法，用于确定受试生物材料如转基因植物材料和对照生物材料如基因未修饰植物材料之间的化学相关差异，该方法至少包括以下六个步骤。 第一步是用流体萃取剂如水，异丙醇和氢氧化钾的混合物接触主题生物材料，以产生受试者生物材料的流体提取物。 第二步是将控制生物材料与流体萃取剂接触以产生对照生物材料的流体萃取物。 第三步是对目标生物材料的流体提取物进行色谱分离，例如气相色谱或流体色谱法，以产生该物质生物材料的液体提取物的色谱图。 第四步是对对照生物材料的流体提取物进行色谱分析以产生对照生物材料的液体提取物的色谱图。 第五步是确定色谱图之间的差异，例如，使用美国专利No. 确定至少一个异常值峰值的第5,592,402号。 第六步是确定离子峰的化学特性，例如使用离子峰的气相色谱/质谱分析。

公开(公告)号：US4793920A

公开(公告)日：1988-12-27

申请号：US111147

申请日：1987-10-20

申请人： Hernan Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter ,  Timothy S. Stevens

发明人： Hernan Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter ,  Timothy S. Stevens

IPC分类号： G01N30/02 ,  G01N30/56 ,  G01N30/60 ,  B01D15/08

CPC分类号： G01N30/56 ,  G01N2030/565 ,  G01N2030/6008 ,  G01N30/02 ,  G01N30/6004 ,  G01N30/6052

摘要： Porous ceramic plugs are cast in place in chromatographic columns in order to provide supports for chromatographic beds in liquid chromatography devices and restrictors in supercritical fluid chromatographic devices. The supports are cast in place by fusing a silicate containing solution, such as one containing potassium silicate, which has been drawn into the outlet end of the column.

摘要翻译： 多孔陶瓷塞在色谱柱中浇注到位，以便为超临界流体色谱装置中液相色谱仪和限流器中的色谱床提供支持。 通过将已经被吸入柱的出口端的含硅酸盐溶液，例如含有硅酸钾的溶液熔化，从而将载体浇注到位。

公开(公告)号：US5240604A

公开(公告)日：1993-08-31

申请号：US982265

申请日：1992-11-25

申请人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Steven J. Martin ,  Charles G. Smith ,  Gary L. Jewett

发明人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Steven J. Martin ,  Charles G. Smith ,  Gary L. Jewett

IPC分类号： G01N30/02 ,  G01N30/12 ,  G01N30/46 ,  G01N30/84 ,  G01N31/12 ,  G01N33/44

CPC分类号： G01N30/463 ,  G01N30/12 ,  G01N30/84 ,  G01N31/12 ,  G01N2030/025 ,  G01N2030/125 ,  G01N2030/8405 ,  G01N30/02 ,  G01N30/462 ,  G01N33/44

摘要： An on-line multidimensional system which includes a liquid chromatograph having an on-line connection to a pyrolysis probe, which in turn has on-line connection to a gas chromatograph. Preferred applications use a size-exclusion chromatograph coupled to a pyrolysis probe coupled to a gas chromatograph to simultaneously produce composition as a function of molecular weight/size information for polymeric materials.

摘要翻译： 一种在线多维系统，其包括具有与热解探针的在线连接的液相色谱仪，其又与气相色谱仪在线连接。 优选的应用使用与耦合到气相色谱仪的热解探针耦合的尺寸排阻色谱，以同时产生作为聚合物材料的分子量/尺寸信息的函数的组成。

公开(公告)号：US5236593A

公开(公告)日：1993-08-17

申请号：US982266

申请日：1992-11-25

申请人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter

发明人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter

IPC分类号： G01N30/02 ,  G01N30/16 ,  G01N30/46

CPC分类号： G01N30/465 ,  G01N2030/025 ,  G01N2030/165 ,  G01N30/462

摘要： Apparatus and method for on-line coupled liquid chromatography and gas chromatography analysis. The system includes a high-volume, liquid vaporizing chamber, or interface capillary tube which allows injection of a substantial volume of sample-carrying eluent and concentrates bands, obviating adverse effects on the resolution or peak shape of the components of interest. The vaporizing chamber is interposed between a packed or wall-coated open tubular capillary liquid chromatographic column and a capillary gas chromatographic column. The capillary liquid chromatographic column permits the use of small volumes of eluent and reduced flow rates.

摘要翻译： 在线耦合液相色谱和气相色谱分析的仪器和方法。 该系统包括大容量的液体蒸发室或接口毛细管，其允许注入大量的样品携带洗脱液和浓缩物带，消除对感兴趣的组分的分辨率或峰形的不利影响。 蒸发室介于填充或壁涂开放的管状毛细管液相色谱柱和毛细管气相色谱柱之间。 毛细管液相色谱柱允许使用少量洗脱液和降低流速。

公开(公告)号：US5522988A

公开(公告)日：1996-06-04

申请号：US318481

申请日：1994-10-05

申请人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter

发明人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter

IPC分类号： G01N30/02 ,  G01N30/16 ,  G01N30/46 ,  B01D15/08

CPC分类号： G01N30/465 ,  G01N2030/025 ,  G01N2030/165 ,  G01N30/462

摘要： Apparatus and method for on-line coupled liquid chromatography and gas chromatography analysis. The system includes a high-volume, liquid vaporizing chamber, or interface capillary tube which allows injection of a substantial volume of sample-carrying eluent and concentrates bands, obviating adverse effects on the resolution or peak shape of the components of interest. The vaporizing chamber is interposed between a packed or wall-coated open tubular capillary liquid chromatographic column and a capillary gas chromatographic column. The capillary liquid chromatographic column permits the use of small volumes of eluent and reduced flow rates.

摘要翻译： 在线耦合液相色谱和气相色谱分析的仪器和方法。 该系统包括大容量的液体蒸发室或接口毛细管，其允许注入大量的样品携带洗脱液和浓缩物带，消除对感兴趣的组分的分辨率或峰形的不利影响。 蒸发室介于填充或壁涂开放的管状毛细管液相色谱柱和毛细管气相色谱柱之间。 毛细管液相色谱柱允许使用少量洗脱液和降低流速。

公开(公告)号：US5376277A

公开(公告)日：1994-12-27

申请号：US68486

申请日：1993-05-28

申请人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Steven J. Martin ,  Charles G. Smith ,  Gary L. Jewett

发明人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Steven J. Martin ,  Charles G. Smith ,  Gary L. Jewett

IPC分类号： G01N30/02 ,  G01N30/12 ,  G01N30/46 ,  G01N30/84 ,  G01N31/12 ,  G01N33/44 ,  B01D15/08

CPC分类号： G01N30/463 ,  G01N30/12 ,  G01N30/84 ,  G01N31/12 ,  G01N2030/025 ,  G01N2030/125 ,  G01N2030/8405 ,  G01N30/02 ,  G01N30/462 ,  G01N33/44

摘要： An on-line multidimensional system which includes a liquid chromatograph having an on-line connection to a pyrolysis probe, which in turn has on-line connection to a gas chromatograph. Preferred applications use a size-exclusion chromatograph coupled to a pyrolysis probe coupled to a gas chromatograph to simultaneously produce composition as a function of molecular weight/size information for polymeric materials.

摘要翻译： 一种在线多维系统，其包括具有与热解探针的在线连接的液相色谱仪，其又与气相色谱仪在线连接。 优选的应用使用与耦合到气相色谱仪的热解探针耦合的尺寸排阻色谱，以同时产生作为聚合物材料的分子量/尺寸信息的函数的组成。

公开(公告)号：US5139681A

公开(公告)日：1992-08-18

申请号：US594403

申请日：1990-10-09

申请人： Hernan J. Cortes ,  Robert M. Campbell ,  R. Paul Himes ,  Curtis D. Pfeiffer

发明人： Hernan J. Cortes ,  Robert M. Campbell ,  R. Paul Himes ,  Curtis D. Pfeiffer

IPC分类号： B01D15/08 ,  G01N30/02 ,  G01N30/06 ,  G01N30/12 ,  G01N30/38 ,  G01N30/46

CPC分类号： G01N30/463 ,  G01N2030/062 ,  G01N2030/126 ,  G01N30/02 ,  G01N30/38 ,  G01N30/462

摘要： An on-line multidimensional chromatographic system and method which couples a liquid chromatograph to a supercritical fluid chromatograph. The system includes an inlet column for depositing a fraction of interest eluting from the liquid chromatograph, an interface interposed between the inlet column and the supercritical fluid chromatograph for receiving and trapping an analyte from the deposited fraction on an impactor surface, and a valve arrangement for controlling fluid flow throughout the system. After the fraction of interest has been deposited in the inlet column, the liquid chromatograph solvent is removed from the inlet column, and then the deposited fraction is extracted from the inlet column in a supercritical fluid stream. The supercritical fluid stream is directed into the interface, where the analyte is trapped while the supercritical fluid is decompressed and vented. A subsequent supercritical fluid stream is then used to introduce the trapped analyte directly into the analytical column of the supercritical fluid chromatograph.

摘要翻译： 一种将液相色谱仪与超临界流体色谱仪相结合的在线多维色谱系统和方法。 该系统包括用于从液相色谱仪洗脱出一部分沉积物的入口柱，介于入口柱和超临界流体色谱仪之间的界面，用于接收和捕获来自沉积部分的分析物在冲击器表面上，以及阀装置 控制流经整个系统的流体。 感兴趣的部分已经沉积在入口塔中后，液体色谱溶剂从入口塔中除去，然后以超临界流体流从入口塔提取沉积的馏分。 将超临界流体流引入界面，在该界面中分析物被捕获，同时超临界流体被减压和排气。 然后使用随后的超临界流体流将捕获的分析物直接引入超临界流体色谱仪的分析柱中。

公开(公告)号：US5679255A

公开(公告)日：1997-10-21

申请号：US902144

申请日：1992-06-22

申请人： Hernan J. Cortes ,  Curtis D. Pfeiffer

发明人： Hernan J. Cortes ,  Curtis D. Pfeiffer

IPC分类号： G01N30/02 ,  G01N30/56 ,  G01N30/60 ,  B01D15/08

CPC分类号： G01N30/56 ,  G01N2030/027 ,  G01N2030/565 ,  G01N30/6052

摘要： Highly efficient microcolumns suitable for use in chromatography, especially size excusion chromatography, are presented along with the method for making them. The method involves first obtaining a microcolumn having a first end and a second end, said microcolumn having an inner diameter of less than 1 mm. Then a slurry is prepared containing a packing material and a liquid solvent, said slurry having a concentration of more than 10 milliliters of solvent per gram of packing material. A retaining means is placed at the second end of the microcolumn, said retaining means being effective for retaining the packing material while allowing the liquid solvent to pass through the retaining means. The slurry is then added to the first end of the microcolumn at a constant pressure, said pressure being less than about 680 atmospheres. After a length of column suitable for use in chromatography has been filled, the addition of the slurry is stopped. The column is allowed to slowly decompress by allowing the solvent to exit the second end of the column.

摘要翻译： 介绍了适用于色谱，特别是大小排放色谱的高效微柱及其制备方法。 该方法首先获得具有第一端和第二端的微柱，所述微柱的内径小于1mm。 然后制备含有包装材料和液体溶剂的浆料，所述浆料的浓度大于10毫升溶剂/克包装材料。 保持装置放置在微柱的第二端，所述保持装置有效地保持包装材料，同时允许液体溶剂通过保持装置。 然后将浆料以恒定压力加入到微柱的第一端，所述压力小于约680个大气压。 在适用于色谱法的一段色谱柱已经填充之后，停止加入浆料。 通过允许溶剂离开色谱柱的第二端，允许色谱柱缓慢减压。

公开(公告)号：US5231466A

公开(公告)日：1993-07-27

申请号：US739278

申请日：1991-08-01

申请人： Steven R. Erskine ,  Hernan J. Cortes ,  Yvonne M. Walbroehl ,  Curtis D. Pfeiffer

发明人： Steven R. Erskine ,  Hernan J. Cortes ,  Yvonne M. Walbroehl ,  Curtis D. Pfeiffer

IPC分类号： G01N21/45 ,  G01N21/85

CPC分类号： G01N21/45 ,  G01N21/4788 ,  G01N21/85

摘要： There is provided an apparatus for monitoring the relative concentration of an analyte in a fluid stream, with such apparatus including a capillary tube for containing the flowing fluid, optics for directing a single beam of monochromatic light through the capillary tube to produce a diffraction pattern having a number of interference fringes. A translatable photodiode is provided to monitor a portion of one of the fringes passing through a light limiting slit, whereby a signal proportional to the concentration of the analyte in the fluid is produced based upon the varying intensity of the monitored fringe. The capillary tube is preferably provided as a fused silica member having an inner flow diameter of 100 micrometers or less.

摘要翻译： 提供了一种用于监测流体流中分析物的相对浓度的装置，其中这种装置包括用于容纳流动流体的毛细管，用于将单个单色光束引导通过毛细管的光学器件，以产生具有 一些干扰条纹。 提供可平移的光电二极管以监测通过光限制狭缝的条纹中的一个的一部分，由此基于所监视的边缘的变化的强度产生与流体中分析物的浓度成比例的信号。 毛细管优选设置为内径为100微米或更小的熔融二氧化硅构件。